Synergetic effect of topological cue and periodic mechanical tension-stress on osteogenic differentiation of rat bone mesenchymal stem cells

Liu, Yao; Yang, Guang; Ji, Hua; Tao, Xiaxin; Luo, En; Zhou, Shaobing

doi:10.1016/j.colsurfb.2017.02.035

Cited by 19 publications

(16 citation statements)

References 58 publications

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“…Mesenchymal stem cells (MSCs) are poly-functional stem cells that normally appear on perivascular regions of various organs [ 1 ]. Moreover, MSCs serve as crucial players in cell-based therapies such as metabolic bone diseases, bone regeneration, and bone reconstruction due to their inherent ability to self-renew and their potential of osteogenic differentiation into tissues of mesenchymal origin [ 2 , 3 ]. Osteogenic differentiation is an intricate biological process that is mediated by both internal cellular signals and external micro-environmental inducements [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-149 suppresses osteogenic differentiation of mesenchymal stem cells via inhibition of AKT1-dependent Twist1 phosphorylation

Fan

Wang

2022

Cell Death Discov.

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Osteogenic differentiation is a vital process for growth, repair, and remodeling of bones. Accumulating evidence have suggested that microRNAs (miRNAs or miRs) play a crucial role in osteogenic differentiation of mesenchymal stem cells (MSCs). Hence, the current study set out to elucidate the role of miR-149 in osteogenic differentiation of MSCs and the underlying mechanism. First, rat models of bone differentiation were established using the Masquelet-induced membrane technique, and MSCs were isolated. The expression of miR-149 and AKT1 in the rats and cells was detected with RT-qPCR and western blot analysis. The relationships among miR-149, AKT1, and Twist1 were further predicted by online bioinformatics prediction and verified using dual luciferase reporter gene assay. Alteration of miR-149, AKT1, or Twist1 was performed to further explore their effect on osteogenic differentiation of MSCs. miR-149 was poorly expressed in the process of osteogenic differentiation of MSCs, while AKT1 was highly expressed. miR-149 negatively regulated the expression of AKT1, which in turn diminished the protein levels of Twist1 and promoted the phosphorylation levels of Twist1. Lastly, miR-149 acted as an inhibitor of osteogenic differentiation of MSCs, which could be reversed by AKT1. To sum up, miR-149 silencing promoted osteogenic differentiation of MSCs by enhancing Twist1 degradation through AKT1 upregulation, representing a new method for bone repair treatment.

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Section: Introductionmentioning

confidence: 99%

MicroRNA-149 suppresses osteogenic differentiation of mesenchymal stem cells via inhibition of AKT1-dependent Twist1 phosphorylation

Fan

Wang

2022

Cell Death Discov.

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“…Bone marrow mesenchymal stem cells (BMSCs) belong to a population of self-renewing multipotent cells and can differentiate along several lineages like osteogenic lineage in response to stimulation by multiple environmental factors and play a critical role in tissue regeneration [1]. Because of their osteogenic potential, BMSCs are able to undergo self-renewal and differentiate into tissues of mesenchymal origin, making them significant contributors during bone repair [2,3]. Moreover, it was reported that BMSC osteogenesis promotes calvarial bone regeneration [4].…”

Section: Introductionmentioning

confidence: 99%

Extracellular vesicle-encapsulated miR-22-3p from bone marrow mesenchymal stem cell promotes osteogenic differentiation via FTO inhibition

et al. 2020

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Background: Bone marrow mesenchymal stem cells (BMSCs) exhibit the capacity to self-renew and differentiate into multi-lineage cell types, including osteoblasts, which are crucial regulators of fracture healing. Thus, this study aims to investigate the effect of microRNA (miR)-22-3p from BMSC-derived EVs on osteogenic differentiation and its underlying mechanism. Methods: Extracellular vesicles (EVs) were isolated from BMSCs and taken up with BMSCs. Dual-luciferase reporter gene assay was used to verify the binding relationship between miR-22-3p and FTO. Loss-and gain-of-function experiments were performed to determine the roles of EV-delivered miR-22-3p and FTO in osteogenic differentiation as well as their regulatory role in the MYC/PI3K/AKT axis. To determine the osteogenic differentiation, ALP and ARS stainings were conducted, and the levels of RUNX2, OCN, and OPN level were determined. In vivo experiment was conducted to determine the function of EV-delivered miR-22-3p and FTO in osteogenic differentiation, followed by ALP and ARS staining. Results: miR-22-3p expression was repressed, while FTO expression was elevated in the ovariectomized mouse model. Overexpression of miR-22-3p, EV-delivered miR-22-3p, increased ALP activity and matrix mineralization of BMSCs and promoted RUNX2, OCN, and OPN expressions in BMSCs. miR-22-3p negatively targeted FTO expression. FTO silencing rescued the suppressed osteogenic differentiation by EV-delivered miR-22-3p inhibitor. FTO repression inactivated the MYC/PI3K/AKT pathway, thereby enhancing osteogenic differentiation both in vivo and in vitro. Conclusion: In summary, miR-22-3p delivered by BMSC-derived EVs could result in the inhibition of the MYC/PI3K/ AKT pathway, thereby promoting osteogenic differentiation via FTO repression.

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“…This section of the review will discuss how electrospun mats with controlled porosity and surface morphology have been used to influence the behavior of mesenchymal stem cells (MSCs) (Jiang et al, 2015 ; Yin et al, 2015 ; Baudequin et al, 2017 ; Lin et al, 2017 ; Liu et al, 2017 ; Nedjari et al, 2017 ; Su et al, 2017 ; Zhang et al, 2017 ; Ghosh et al, 2018 ; Jin et al, 2018 ; Rahman et al, 2018 ; Sankar et al, 2018 ) and human umbilical vein endothelial cells (HUVECs) (Fioretta et al, 2014 ; Xu et al, 2015 ; Shin et al, 2017 ; Taskin et al, 2017 ; Yan et al, 2017 ; Ahmed et al, 2018 ). The literature on other cell lines, such as on myoblasts (Mele et al, 2015 ; Jun et al, 2016 ; Park et al, 2016 ; Tallawi et al, 2016 ; Abarzúa-Illanes et al, 2017 ; Yang et al, 2017 ) and neuron-like cells (Binan et al, 2014 ; Xie et al, 2014 ; Malkoc et al, 2015 ; Xue et al, 2017 ; Hajiali et al, 2018 ; Xia and Xia, 2018 ), will not be analyzed in detail here but a summary of it is reported in Table 1 .…”

Section: Effects Of Fiber Topography and Micro-patterning On Cellularmentioning

confidence: 99%

Cellular Response to Surface Morphology: Electrospinning and Computational Modeling

Denchai

Tartarini

Mele

2018

Front. Bioeng. Biotechnol.

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Surface properties of biomaterials, such as chemistry and morphology, have a major role in modulating cellular behavior and therefore impact on the development of high-performance devices for biomedical applications, such as scaffolds for tissue engineering and systems for drug delivery. Opportunely-designed micro- and nanostructures provides a unique way of controlling cell-biomaterial interaction. This mini-review discusses the current research on the use of electrospinning (extrusion of polymer nanofibers upon the application of an electric field) as effective technique to fabricate patterns of micro- and nano-scale resolution, and the corresponding biological studies. The focus is on the effect of morphological cues, including fiber alignment, porosity and surface roughness of electrospun mats, to direct cell migration and to influence cell adhesion, differentiation and proliferation. Experimental studies are combined with computational models that predict and correlate the surface composition of a biomaterial with the response of cells in contact with it. The use of predictive models can facilitate the rational design of new bio-interfaces.

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Synergetic effect of topological cue and periodic mechanical tension-stress on osteogenic differentiation of rat bone mesenchymal stem cells

Cited by 19 publications

References 58 publications

MicroRNA-149 suppresses osteogenic differentiation of mesenchymal stem cells via inhibition of AKT1-dependent Twist1 phosphorylation

MicroRNA-149 suppresses osteogenic differentiation of mesenchymal stem cells via inhibition of AKT1-dependent Twist1 phosphorylation

Extracellular vesicle-encapsulated miR-22-3p from bone marrow mesenchymal stem cell promotes osteogenic differentiation via FTO inhibition

Cellular Response to Surface Morphology: Electrospinning and Computational Modeling

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