Synergetic alginate conversion by a microbial consortium of hydrolytic bacteria and methanogens

Zhang, Fang; Zhang, Wei; Qian, Ding-Kang; Keino, Dai; Loosdrecht, Mark C.M. van; Zeng, Raymond J.

doi:10.1016/j.watres.2019.114892

Cited by 40 publications

(57 citation statements)

References 53 publications

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“…The characteristics, including pH, total COD, soluble COD, suspended solids, and volatile suspended solids (VSS), are summarized in Table S1. The ADC biomass was collected from a mesophilic (35 °C) anaerobic reactor fed with alginate . Another methanogenic culture (MC) was set as a control culture and was collected from a lab-scale anaerobic digester …”

Section: Methodsmentioning

confidence: 99%

“…Mixed cultures provide several advantages for the utilization of WAS over pure cultures because there is no requirement for sterilization, and it can be adapted to variation in feedstocks and process conditions . Recently, we enriched alginate-degrading consortia (ADC) using alginate as a substrate, which demonstrated a high activity of alginate conversion to methane (>80%) . A metagenomics analysis showed that 12 species, such as Algibacter lectus and Bacteroides clarus, could excrete an oligo-alginate lyase to degrade alginate .…”

Section: Introductionmentioning

confidence: 99%

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Enhanced Methane Recovery from Waste-Activated Sludge by Alginate-Degrading Consortia: The Overlooked Role of Alginate in Extracellular Polymeric Substances

Zhang

Qian

Geng

et al. 2020

Environ. Sci. Technol. Lett.

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The hydrolysis of extracellular polymeric substances (EPS) in waste-activated sludge (WAS) is considered as the rate-limiting step in anaerobic digestion. Uronic acids such as alginate are one of main polysaccharide components in EPS; however, their roles on WAS conversion are overlooked until now. Previously, we described alginatedegrading consortia (ADC) that have high activity for alginate conversion. In this work, ADC was studied for polysaccharide hydrolysis and methane production from WAS for the first time, which increased the methane production by 115%−185%. Dosing ADC also increased the values of biological methane potential from 131 to 172 mL/gVSS. An alginate-like exopolysaccharide was extracted from WAS, and the content was 65 mg/ g-VSS. Then, the molecular weight profiles at UV 254nm showed that disaccharides were the final hydrolysates of alginate by ADC enzyme. Extracted EPS could be utilized by ADC for methane production with acetate as the main intermediate. The mechanism was proposed that ADC played a key role in WAS conversion. These results indicated that alginate in EPS shall not be overlooked, which offers a new microbial method to enhance methane recovery from WAS. The microbial changes in ADC for the stability of WAS digestion should be investigated in the future.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Enhanced Methane Recovery from Waste-Activated Sludge by Alginate-Degrading Consortia: The Overlooked Role of Alginate in Extracellular Polymeric Substances

Zhang

Qian

Geng

et al. 2020

Environ. Sci. Technol. Lett.

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“…Acetate and hydrogen are major metabolites of genus Lentimicrobium within Lentimicrobiaceae family (Zhang et al . 2019). Most members of the phylum Chloroflexi are anaerobic acetogenic microbes (Liang et al .…”

Section: Discussionmentioning

confidence: 99%

“…Methanosaeta is an aceticlastic methanogen (Shahimin and Siddique 2017; Zhang et al . 2019), degrading acetate via aceticlastic pathway. Species of M. beijingense (hydrogenotrophic methanogen) is able to degrade acetate via interspecies hydrogen transfer (IHT) (Ma et al .…”

Section: Discussionmentioning

confidence: 99%

Microbially induced carbonate precipitation via methanogenesis pathway by a microbial consortium enriched from activated anaerobic sludge

Yang

2020

J. Appl. Microbiol.

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Aims Various applications of microbially induced carbonate precipitation (MICP) has been proposed. However, most studies use cultured pure strains to obtain MICP, ignoring advantages of microbial consortia. The aims of this study were to: (i) test the feasibility of a microbial consortium to produce MICP; (ii) identify functional micro‐organisms and their relationship; (iii) explain the MICP mechanism; (iv) propose a way of applying the MICP technique to soil media. Methods and Results Anaerobic sludge was used as the source of the microbial consortium. A laboratory anaerobic sequencing batch reactor and beaker were used to perform precipitation experiment. The microbial consortium produced MICP with an efficiency of 96·6%. XRD and SEM analysis showed that the precipitation composed of different‐size calcite crystals. According to high‐throughput 16S rRNA gene sequencing, the functional micro‐organisms included acetogenic bacteria, acetate‐oxidizing bacteria and archaea Methanosaeta and Methanobacterium beijingense. The methanogenesis acetate degradation provides dissolved inorganic carbon and increases pH for MICP. A series of reactions catalysed by many enzymes and cofactors of methanogens and acetate‐oxidizers are involved in the acetate degradation. Conclusion This work demonstrates the feasibility of using the microbial consortium to achieve MICP from an experimental and theoretical perspective. Significance and Impact of the Study A method of applying the microbial‐consortium MICP to soil media is proposed. It has the advantages of low cost, low environmental impact, treatment uniformity and less limitations from natural soils. This method could be used to improve mechanical properties, plug pores and fix harmful elements of soil media, etc.

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“…High-Throughput Sequencing and Statistical Analysis. After 25 days of incubation, we collected samples from G5, H5, F5, and G0 for microbial community analysis through the extraction of total microbial DNA from the samples 28 by using MOBIO/QIAGEN Soil Microbial DNA Extraction Kit. The amount of OC within these samples was similar to that of background values in the collected soil.…”

Section: Methodsmentioning

confidence: 99%

Impacts of External Organic Carbon on Arsenic Release in Aquifer of Jianghan Plain, Central China

Sun

Lan

Chen

et al. 2021

ACS Earth Space Chem.

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This study investigated the effects of organic carbon, e.g., glucose, humic acid, and fulvic acid, on the As enrichment of groundwater from an As-bearing aquifer under the Jianghan Plain, China. Sediments were obtained by drilling samples and cultured in the laboratory by adding exotic organic matter. On the basis of high-throughput sequencing, it can be found that the addition of organic matter causes the change of microbial community diversity in the sediments. We found the co-occurrence of organic carbon and indigenous microfloral assemblage (family Clostridiaceae) promoted the transfer of As to a liquid phase by dissolving and destroying Fe oxides. Through XPS, XRD, and EXAFS analyses of sediments in different periods, we can conclude that, in this process, As was converted into labile and unstable intermediates (As 2 S 3 , As 2 S 5 , or AsS) through the degradation of organic carbon. These novel results provide valuable information for the remediation and risk control of As-contaminated sites.

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Synergetic alginate conversion by a microbial consortium of hydrolytic bacteria and methanogens

Cited by 40 publications

References 53 publications

Enhanced Methane Recovery from Waste-Activated Sludge by Alginate-Degrading Consortia: The Overlooked Role of Alginate in Extracellular Polymeric Substances

Enhanced Methane Recovery from Waste-Activated Sludge by Alginate-Degrading Consortia: The Overlooked Role of Alginate in Extracellular Polymeric Substances

Microbially induced carbonate precipitation via methanogenesis pathway by a microbial consortium enriched from activated anaerobic sludge

Impacts of External Organic Carbon on Arsenic Release in Aquifer of Jianghan Plain, Central China

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