1995
DOI: 10.1104/pp.107.3.703
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Synechococcus sp. PCC7942 Transformed with Escherichia coli bet Genes Produces Glycine Betaine from Choline and Acquires Resistance to Salt Stress

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Cited by 115 publications
(64 citation statements)
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“…The plasmids pUC303-KatE (15), pUC303-Bet (13), and pUC303-KatE͞Bet (15) were used for the expression of mono-functional catalase, betaine, and both catalase and betaine, respectively. The plasmid pUC303-Bm was used in the control cells (13). For the construction of expression plasmid pUC303-DnaK, the 3-kbp fragment that contains the dnaK gene from A. halophytica (20) was digested with SspI and EcoRV and blunt-ended, and then ligated into the EcoRI-and SalI-double-digested and blunt-ended site of pUC303-Bm.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…The plasmids pUC303-KatE (15), pUC303-Bet (13), and pUC303-KatE͞Bet (15) were used for the expression of mono-functional catalase, betaine, and both catalase and betaine, respectively. The plasmid pUC303-Bm was used in the control cells (13). For the construction of expression plasmid pUC303-DnaK, the 3-kbp fragment that contains the dnaK gene from A. halophytica (20) was digested with SspI and EcoRV and blunt-ended, and then ligated into the EcoRI-and SalI-double-digested and blunt-ended site of pUC303-Bm.…”
Section: Methodsmentioning
confidence: 99%
“…PCC 7942 (13)(14)(15). However, the expression of a Na ϩ ͞H ϩ antiporter from Vibrio alginolytica NhaAv resulted in a Na ϩ -sensitive transformant (16).…”
Section: Aphanothece Halophytica ͉ Genetic Engineeringmentioning
confidence: 99%
“…This effect has been observed in tobacco after the transfer of genes or cDNAs encoding enzymes that lead to the biosynthesis of mannitol, Gly betaine, Pro, trehalose, or fructan (Tarczynski et al, 1993;Kishor et al, 1995;Nomura et al, 1995;Pilon-Smits et al, 1995;Holmstrom et al, 1996). Because many naturally salt-or drought-tolerant plants accumulate such compounds when stressed, these metabolites are considered to be compatible solutes that act by providing osmotic adjustment and by lowering the osmotic potential, i.e.…”
mentioning
confidence: 99%
“…After 1 week of treatment, samples were taken for quantification of glycinebetaine accumulated in the leaves. Quantification of glycinebetaine was carried out as previously reported (Nomura et aL, 1995).…”
Section: Metabolism Of Exogenously-added Betaine Aldehydementioning
confidence: 99%