Syndrome de Turner et isoformes de LH et FSH. Intérêt des techniques enzymatiques polyclonales

Pétrus, M; Rittie, J. L.; Causse, J; Moulie, N.; Rhabbour, M.; Netter, J. C.; Hirsch, C.; Bildstein, G

doi:10.1016/0929-693x(96)81302-9

Cited by 1 publication

(1 citation statement)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As some PAs secrete different hormone isoforms, they cannot be recognised by specific antibodies. Indeed, Petrus et al demonstrated that analytical methods using monoclonal antibodies could underestimate FSH levels when the balance of FSH isoforms varied [19]. That could explain why 33 tumours molecularly identified as GT (71.7% of the M-GT), were diagnosed immunohistochemically as NC or other subtypes in our series.…”

Section: Discussionmentioning

confidence: 83%

Contribution of molecular analysis to the typification of the non-functioning pituitary adenomas

et al. 2017

View full text Add to dashboard Cite

AimThe WHO Classification of Tumours of Endocrine Organs considers the inmunohistochemical characterization of pituitary adenomas (PA) as mandatory for patient diagnosis. Recent advances in the knowledge of the molecular patterns of these tumours could complement this classification with gene expression profiling.MethodsWithin the context of the Spanish Molecular Registry of Pituitary Adenomas (REMAH), a multicentre clinical-basic research project, we analysed the molecular phenotype of 142 PAs with complete IHC and clinical information. Gene expression levels of all pituitary hormones, type 1 corticotrophin-releasing hormone receptor, dopamine receptors and arginine vasopressin receptor 1b were measured by quantitative real-time polymerase chain reaction. In addition, we used three housekeeping genes for normalization and a pool of nine healthy pituitary glands from autopsies as calibration reference standard.ResultsBased on the clinically functioning PA (FPA: somatotroph, corticotroph, thyrotroph and lactotroph adenomas), we established the interquartile range of relative expression for all genes studied in each PA subtype. That allowed molecularly the different PA subtypes, including the clinically non-functioning PA (NFPA). Afterwards, we estimated the concordance of the molecular and immunohistochemical classification with clinical diagnosis in FPA and between them in NFPA. The kappa values were higher in molecular than in immunohistochemical classification in FPA and showed a bad concordance in all NFPA subtypes.ConclusionsAccording to these results, the molecular characterization of the PA complements the IHC analysis, allowing a better typification of the NFPA.

show abstract

Section: Discussionmentioning

confidence: 83%