Syndecan-1 responsive microRNA-126 and 149 regulate cell proliferation in prostate cancer

Fujii, Tomomi; Shimada, Kaoru; Tatsumi, Yoshihiro; Fujimoto, Kiyohide; Konishi, Noboru

doi:10.1016/j.bbrc.2014.11.056

Cited by 44 publications

(45 citation statements)

References 42 publications

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“…Furthermore, the ability of miRNAs to reprogram cancer cells into cells with stem cell-like properties and low malignancy potential has been previously demonstrated in colon cancer [23], therefore miRNAs could regulate the fate of cancer cells. These results are in line with our recently published study, in which we demonstrate that syndecan-1, miR-126, and miR-149 modulate cell senescence by controlling expression of SOX2, NANOG, and OCT4 [13].…”

Section: Discussionsupporting

confidence: 93%

“…We previously reported that this proteoglycan transforms androgen-dependent prostate cancer cells into androgen-independent tumors and facilitates progression [11,12]. Moreover, syndecan-1 and the miRNAs miR-126 and, 149 regulate cell proliferation through down-regulation of several stem cell factors such as SOX2, NANOG, and OCT4 [13]. Finally, syndecan-1 was detected in plasmacytoid urothelial carcinoma, an aggressive tumor [14][15][16][17][18], demonstrating that the protein is closely associated with urothelial carcinoma progression [19].…”

Section: Introductionmentioning

confidence: 94%

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microRNA-145 promotes differentiation in human urothelial carcinoma through down-regulation of syndecan-1

et al. 2016

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Background: A new molecular marker of carcinoma in the urinary bladder is needed as a diagnostic tool or as a therapeutic target. Potential markers include microRNAs (miRNAs), which are short, low molecular weight RNAs 19-24 nt long that regulate genes associated with cell proliferation, differentiation, and development in various cancers. In this study, we investigated the molecular mechanisms by which miR-145 promotes survival of urothelial carcinoma cells and differentiation into multiple lineages. We found miR-145 to regulate expression of syndecan-1, a heparin sulfate proteoglycan. Methods: Cell proliferation in the human urothelial carcinoma cell lines T24 and KU7 was assessed by MTS assay. Cellular senescence and apoptosis were measured by senescence-associated β-galactosidase (SA-β-gal) and TUNEL assay, respectively. Quantitative RT-PCR was used to measure mRNA expression of various genes, including syndecan-1, stem cell factors, and markers of differentiation into squamous, glandular, or neuroendocrine cells.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 94%

microRNA-145 promotes differentiation in human urothelial carcinoma through down-regulation of syndecan-1

et al. 2016

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“…The gene serves as an anti-oncogene in colorectal cancer [35], HCC [36], laryngeal squamous cell carcinoma [37], and renal cell carcinoma [38]. In contrast, the gene functioned as an oncogene gene in T-cell acute lymphoblastic leukemia [39] and prostate cancer [40]. Hence, we may infer that hsa-mir-149 plays completely different roles across different tumor types.…”

Section: Discussionmentioning

confidence: 89%

Identifying Differentially Expressed MicroRNAs Between Cirrhotic and Non-Cirrhotic Hepatocellular Carcinoma and Exploring Their Functions Using Bioinformatic Analysis

Mei¹,

You²,

Jin³

et al. 2018

Cell Physiol Biochem

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Background/Aims: Few studies have been designed to directly investigate the exact mechanisms underlying the different phenotypes between cirrhotic and non-cirrhotic hepatocellular carcinoma (HCC). This study aimed to illuminate the incidence and developmental mechanisms for both types of HCC through differentially expressed microRNAs (miRNAs) using bioinformatic analysis. Methods: The miRNA-seq data and clinical data of patients (from The Cancer Genome Atlas (TCGA) database) were utilized to determine differentially expressed miRNAs between cirrhotic and non-cirrhotic HCC. Afterwards, the function of the selected miRNAs was predicted with online tools. Furthermore, the miRNA expression and clinical significance were validated by external datasets and our experiment. Results: The present study included 135 non-cirrhotic and 80 cirrhotic patients to find differentially expressed miRNAs. Among them, four miRNAs (mir-1296, mir-23c, mir-149, and mir-95) were finally selected for further validation and analysis. Correlation analysis found that two miRNAs are greatly associated with the non-cirrhotic HCC patients’ clinical traits, such as the T, M, and N tumor stages. However, only mir-23c was associated with the cirrhotic HCC patients’ tumor T and N stages. Furthermore, survival analysis revealed that increased mir-149 in non-cirrhotic HCC, patients’ age and the existence of vessels in the tumor in cirrhotic HCC were independent risk factors for the patients’ postoperative overall survival. Functional and interaction analyses also supported the notion that these miRNAs function through some pathways that influence the behavior of the HCC. These results are strongly supported by analysis of extra datasets and our experiment. Conclusions: The cirrhotic and non-cirrhotic HCC types involve differentially expressed miRNAs, which are correlated with distinctive pathological traits. To some extent, non-cirrhotic HCC seems more dependent on miRNA network regulation, but additional studies are needed to confirm this conclusion.

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“…Notably, miR-149-5p promotes cell proliferation. 27 Recent study revealed that miR-149-5p expression is significantly downregulated and plays an important role as a tumor suppressor 28 in some cancers, such as gastric cancer, 29 astrocytomas, 30 glioma, 31 colon, and rectum carcinoma (colorectal cancer (CRC)). 32 Overexpression of miR-149-5p was found to inhibit the viability and invasion of cells in vitro and have prognostic and therapeutic implications on CRC.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide profiling of micro-RNA expression in gefitinib-resistant human lung adenocarcinoma using microarray for the identification of miR-149-5p modulation

Qin

Yan

et al. 2017

Tumour Biol.

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To understand the mechanism involved in gefitinib resistance, we established gefitinib-resistant human HCC827/GR-8-1 cell line from the parental HCC827 cell line. We compared the micro-RNA expression profiles of the HCC827 cells HCC827/GR-8-1 using Agilent micro-RNA microarrays. The micro-RNAs, such as the miR-149-5p, were up-or downregulated and associated with acquired gefitinib resistance. Quantitative real-time polymerase chain reaction was then performed to verify the expression patterns of different micro-RNAs. The result showed that miR-149-5p was upregulated in the HCC827/GR-8-1 cell line. To investigate the biological function of miR-149-5p in non-small cell lung cancer cells acquired gefitinib resistance, we examined cell proliferation using a cell counting kit-8 assay. Cell viability was evaluated after the miR-149-5p mimics, inhibitors, and negative control were separately transfected into the non-small cell lung cancer cells. The results showed that the non-small cell lung cancer cells transfected with miR-149-5p mimics exhibited reduced cell motility. The drug-sensitivity assay results revealed that the overexpression of miR-149-5p effectively evaluates the half maximal inhibitory concentration values of the cell in response to gefitinib, and the downregulation of miR-149-5p can attenuate the half maximal inhibitory concentration values of the cell lines in response to gefitinib. Furthermore, the levels of miR-149-5p in the HCC827 and HCC827/GR-8-1 cells were inversely correlated with caspase-3 expression. In conclusion, this study revealed that miR-149-5p is upregulated in the HCC827/ GR-8-1 cells and involved in the acquired gefitinib resistance.

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Syndecan-1 responsive microRNA-126 and 149 regulate cell proliferation in prostate cancer

Cited by 44 publications

References 42 publications

microRNA-145 promotes differentiation in human urothelial carcinoma through down-regulation of syndecan-1

microRNA-145 promotes differentiation in human urothelial carcinoma through down-regulation of syndecan-1

Identifying Differentially Expressed MicroRNAs Between Cirrhotic and Non-Cirrhotic Hepatocellular Carcinoma and Exploring Their Functions Using Bioinformatic Analysis

Genome-wide profiling of micro-RNA expression in gefitinib-resistant human lung adenocarcinoma using microarray for the identification of miR-149-5p modulation

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