Syncytin-2 Plays an Important Role in the Fusion of Human Trophoblast Cells

Vargas, Amandine; Moreau, Julie; Landry, Sébastien; LeBellego, Frédérique; Toufaily, Chirine; Rassart, Éric; Lafond, Julie; Barbeau, Benoı̂t

doi:10.1016/j.jmb.2009.07.025

Cited by 161 publications

(158 citation statements)

References 39 publications

(52 reference statements)

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“…In agreement with previously published results (Esnault et al, 2008;Knerr et al, 2005;Kudo and Boyd, 2002;Vargas et al, 2009) forskolin treatment of BeWo cells for 24h induced substantial mRNA up-regulation of a cassette of genes encoding fusogenic proteins such as syncytin-1 and -2, MFSD2, OASIS and GCMa (by 3-, 10-, 2-, 3-and 6-fold, respectively), without affecting TORC1 mRNA levels. The mRNA levels of the syncytin-1 receptor, ASCT2, were slightly reduced by 15-20%, (data not shown).…”

Section: Contribution Of Mapk Cascades In Bewo Fusogenic Gene Up-regusupporting

confidence: 92%

Interplay of cAMP and MAPK pathways in hCG secretion and fusogenic gene expression in a trophoblast cell line

Delidaki

Hein

et al. 2011

Molecular and Cellular Endocrinology

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. Interplay of cAMP and MAPK pathways in hCG secretion and fusogenic gene expression in a trophoblast cell line. Molecular and Cellular Endocrinology, Elsevier, 2010, 332 (1-2) This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.Page 1 of 32A c c e p t e d M a n u s c r i p t respectively) the transcription factors old astrocyte specifically-induced substance (OASIS) and glial cells missing a (GCMa) (by 3 and 6 fold, respectively) and the syncytin-2 receptor, major facilitator superfamily domain containing 2 (MFSD2) (by 2 fold). Up-regulation of AKAP79 and AKAP250, (by 2.5 and 4 fold, respectively) was also identified in forskolin-treated BeWo cells. Forskolin effects on all these genes were suppressed by chemical inhibition of p38MAPK whereas only specific genes were sensitive to ERK1/2 inhibition. This data provide novel insights into the signalling molecules and mechanisms regulating fusogenic gene expression by the adenylyl cyclase pathway.

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Section: Contribution Of Mapk Cascades In Bewo Fusogenic Gene Up-regusupporting

confidence: 92%

Interplay of cAMP and MAPK pathways in hCG secretion and fusogenic gene expression in a trophoblast cell line

Delidaki

Hein

et al. 2011

Molecular and Cellular Endocrinology

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“…S2 ). It has been reported that upregulation of GCM1 , syncytin-1 and syncytin-2 are required for FK-induced fusion of BeWo cells 29,42 . We therefore examined whether β -catenin / BCL9L / TCF signalling is required for FK-induced fusion of BeWo cells.…”

Section: O C K S I C O N T R O L S I -C a T -1 M O C K S I C O N T mentioning

confidence: 99%

Identification of a link between Wnt/β-catenin signalling and the cell fusion pathway

et al. 2011

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Cell fusion has a critical role in various developmental processes, immune response, tissue homeostasis and regeneration, and possibly, in cancer. However, the signals that regulate cell fusion remain poorly understood. In a screen for novel targets of Wnt / β -catenin signalling, we identifi ed glial cells missing 1 ( GCM1 ), which encodes a transcription factor that is involved in epigenetic regulation and is critical for the fusion of syncytiotrophoblast (ST) cells. Here we show that β -catenin / BCL9-Like (BCL9L) / T-cell factor 4 (TCF4) signalling directly targets the GCM1 / syncytin pathway and thereby regulates the fusion of human choriocarcinoma cells. Furthermore, we show that the GCM1 / syncytin-B pathway is signifi cantly downregulated in the placenta of BCL9L-defi cient mice and that the fusion and differentiation of ST-II cells are blocked. Our results demonstrate a signal transduction pathway that regulates cell fusion, and may provide intriguing perspectives into the various biological and pathological processes that involve cell fusion.

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“…When the expression of nephrin was knocked down, trophoblast syncytialization as well as the expression of syncytin 2 and connexin 43 was decreased. In the placenta, syncytin is located on the cell membrane and mediates placental CTB cell fusion by binding to the syncytin receptor (Mi et al 2000, Esnault et al 2008, Huppertz & Borges 2008, Vargas et al 2009). As a gap junctional protein, connexin 43 is required for CTB cell fusion into syncytia (Dunk et al 2012, Pidoux et al 2014.…”

Section: Discussionmentioning

confidence: 99%

Involvement of nephrin in human placental trophoblast syncytialization

Li¹,

Zheng²,

Wang³

et al. 2015

Reproduction

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The placenta has numerous functions, such as transporting oxygen and nutrients and building the immune tolerance of the fetus. Cell fusion is an essential process for placental development and maturation. In human placental development, mononucleated cytotrophoblast (CTB) cells can fuse to form a multinucleated syncytiotrophoblast (STB), which is the outermost layer of the placenta. Nephrin is a transmembrane protein that belongs to the Ig superfamily. Previous studies have shown that nephrin contributes to the fusion of myoblasts into myotubes in zebrafish and mice, presenting a functional conservation with its Drosophila ortholog sticks and stones. However, whether nephrin is involved in trophoblast syncytialization remains unclear. In this study, we report that nephrin was localized predominantly in the CTB cells and STB of human placenta villi from first trimester to term pregnancy. Using a spontaneous fusion model of primary CTB cells, the expression of nephrin was found to be increased during trophoblast cell fusion. Moreover, the spontaneous syncytialization and the expression of syncytin 2, connexin 43, and human chorionic gonadotropin beta were significantly inhibited by nephrin-specific siRNAs. The above results demonstrate that nephrin plays an important role in trophoblast syncytialization.

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Syncytin-2 Plays an Important Role in the Fusion of Human Trophoblast Cells

Cited by 161 publications

References 39 publications

Interplay of cAMP and MAPK pathways in hCG secretion and fusogenic gene expression in a trophoblast cell line

Interplay of cAMP and MAPK pathways in hCG secretion and fusogenic gene expression in a trophoblast cell line

Identification of a link between Wnt/β-catenin signalling and the cell fusion pathway

Involvement of nephrin in human placental trophoblast syncytialization

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