Synciironous changes of c-fos RNA and B2+mRNAx concentrations during prereplicative period in regenerating rat liver

Abstract

Исследовалась представленность РНК протоонкогена c-fos а мРНК, содержащей повтор 132 (В2+мРНКх) β ядерной РНК и β цитоплазматической поли(А) [ РНК клеток регенерирующей печени β период 0-12 ч после частичной гепатэктомии. Судя по результатам гибридизации ДНК-РНК, транскрипты c-fos сосредоточены β основном в ядре и концентрация их максимальна к 0,5 и 12 ч регенерации. Напротив, В2 •мРНК^ сосредоточена в поли(А)+РНК цитоплазмы и в те же сроки имеет минимальную кон-!{ентрицию. Различия в экспрессии исследованных генов могут определяться функциональными особенностями кодируемых ими продуктов.

Radiation-induced peculiarities of cytochrome P-4502E1 and oncogenes mRNA accumulation in different rat tissues

“…The connection between the accumulation of c-fos mRNA and Р-450ІІЕІ mRNA (B2mRNA x , CYP2E1 mRNA) during the prereplicative period of rat liver cells division has been shown in previous work [1]. Now we are interested in relationship between on cogenes (c-fos and c-myc) and CYP2E1 gene exp ression under radiation influence which takes place in Chernobyl's exclusion zone.…”

“…The experiments repor ted here were designed to determine whether the expression of different cellular oncogenes as well as cytochrome P-450 genes is altered tn rats following the long exposure to radioactive substances absorbed by inhalation and feeding in Chernobyl's area. Pre vious work from our laboratory has shown that the dynamics of accumulation of c-fos mRNA is strongly related with that of P-450 2E1 mRNA during the prereplicative period of rat liver cells division [1]. Experiments by other groups have shown modulation of c-fos mRNA following exposure of cells in culture to X-rays or other DNA-damaging agents [11][12][13], possibly leading to cell transformation and neoplasia.…”