Synchrotron X-ray microscopy reveals early calcium and iron interaction with crocidolite fibers in the lung of exposed mice

Pascolo, Lorella; Zabucchi, Giuliano; Gianoncelli, Alessandra; Kourousias, George; Trevisan, Elisa; Pascotto, Ernesto; Casarsa, Claudia; Ryan, C.G.; Lucattelli, Monica; Lungarella, Giuseppe; Cavarra, Eleonora; Bartalesi, Barbara; Zweyer, Marina; Cammisuli, Francesca; Melato, Mauro; Borelli, Violetta

doi:10.1016/j.toxlet.2015.11.016

Cited by 15 publications

(11 citation statements)

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“…In the case of asbestos fibres, it has been demonstrated that the relation between asbestos pathogenicity and iron is not only due to the presence of a reactive metal on fibre surfaces, but also, and more importantly, to the fact that asbestos causes a real alteration in the iron homeostasis of the cells, interacting with the fibres 32 – 34 . In line with these observations, by using synchrotron radiation X-ray fluorescence microscopy (SR-XRF) we previously showed that iron and other elements are involved in the response of lung tissue to asbestos, in particular we revealed that the asbestos causes a continuous mobilization of iron from the surrounding cells, mainly alveolar macrophages 35 , 36 .…”

Section: Introductionsupporting

confidence: 55%

Iron-related toxicity of single-walled carbon nanotubes and crocidolite fibres in human mesothelial cells investigated by Synchrotron XRF microscopy

Cammisuli

Giordani

Gianoncelli

et al. 2018

Sci Rep

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Carbon nanotubes (CNTs) are promising products in industry and medicine, but there are several human health concerns since their fibrous structure resembles asbestos. The presence of transition metals, mainly iron, in the fibres seems also implicated in the pathogenetic mechanisms. To unravel the role of iron at mesothelial level, we compared the chemical changes induced in MeT-5A cells by the exposure to asbestos (crocidolite) or CNTs at different content of iron impurities (raw-SWCNTs, purified- and highly purified-SWCNTs). We applied synchrotron-based X-Ray Fluorescence (XRF) microscopy and soft X-ray imaging (absorption and phase contrast images) to monitor chemical and morphological changes of the exposed cells. In parallel, we performed a ferritin assay. X-ray microscopy imaging and XRF well localize the crocidolite fibres interacting with cells, as well as the damage-related morphological changes. Differently, CNTs presence could be only partially evinced by low energy XRF through carbon distribution and sometimes iron co-localisation. Compared to controls, the cells treated with raw-SWCNTs and crocidolite fibres showed a severe alteration of iron distribution and content, with concomitant stimulation of ferritin production. Interestingly, highly purified nanotubes did not altered iron metabolism. The data provide new insights for possible CNTs effects at mesothelial/pleural level in humans.

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Section: Introductionsupporting

confidence: 55%

Iron-related toxicity of single-walled carbon nanotubes and crocidolite fibres in human mesothelial cells investigated by Synchrotron XRF microscopy

Cammisuli

Giordani

Gianoncelli

et al. 2018

Sci Rep

Self Cite

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“…Lazaroids are a group of non-glucocorticoid analogs of methylprednisolone and they have the capacity to cross hydrophobic areas of the cell membrane, particularly to avert membrane LPO [ 261 , 273 , 274 ]. Their protective actions have already been demonstrated in numerous animal models of lung injury [ 274 , 275 ]. Lazaroids mainly exert their protective actions by suppressing LPO.…”

Section: Strategies For Reducing Oxidative Stress By Antioxidants In ...mentioning

confidence: 99%

Therapeutic Potential of Small Molecules Targeting Oxidative Stress in the Treatment of Chronic Obstructive Pulmonary Disease (COPD): A Comprehensive Review

Dailah

2022

Molecules

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Chronic obstructive pulmonary disease (COPD) is an increasing and major global health problem. COPD is also the third leading cause of death worldwide. Oxidative stress (OS) takes place when various reactive species and free radicals swamp the availability of antioxidants. Reactive nitrogen species, reactive oxygen species (ROS), and their counterpart antioxidants are important for host defense and physiological signaling pathways, and the development and progression of inflammation. During the disturbance of their normal steady states, imbalances between antioxidants and oxidants might induce pathological mechanisms that can further result in many non-respiratory and respiratory diseases including COPD. ROS might be either endogenously produced in response to various infectious pathogens including fungi, viruses, or bacteria, or exogenously generated from several inhaled particulate or gaseous agents including some occupational dust, cigarette smoke (CS), and air pollutants. Therefore, targeting systemic and local OS with therapeutic agents such as small molecules that can increase endogenous antioxidants or regulate the redox/antioxidants system can be an effective approach in treating COPD. Various thiol-based antioxidants including fudosteine, erdosteine, carbocysteine, and N-acetyl-L-cysteine have the capacity to increase thiol content in the lungs. Many synthetic molecules including inhibitors/blockers of protein carbonylation and lipid peroxidation, catalytic antioxidants including superoxide dismutase mimetics, and spin trapping agents can effectively modulate CS-induced OS and its resulting cellular alterations. Several clinical and pre-clinical studies have demonstrated that these antioxidants have the capacity to decrease OS and affect the expressions of several pro-inflammatory genes and genes that are involved with redox and glutathione biosynthesis. In this article, we have summarized the role of OS in COPD pathogenesis. Furthermore, we have particularly focused on the therapeutic potential of numerous chemicals, particularly antioxidants in the treatment of COPD.

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“…External pollutants, such as asbestos and cigarette smoke, increase the iron loads in the lungs (Ghio et al, 2008;Pascolo et al, 2016), induce oxidative stress and inflammation, tipping the point towards cancer development. In this context, alterations of genes involved in iron metabolism could either increase or decrease the toxicity of this metal thus possibly either inducing or protecting from the neoplastic transformation.…”

Section: Discussionmentioning

confidence: 99%

Pleural mesothelioma and lung cancer: the role of asbestos exposure and genetic variants in selected iron metabolism and inflammation genes

Celsi

Crovella

Moura

et al. 2019

Journal of Toxicology and Environmental Health, Part A

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Two of the major cancerous diseases associated with asbestos exposure are malignant pleural mesothelioma (MPM) and lung cancer (LC). In addition to asbestos exposure, genetic factors have been suggested as involved in asbestos-related carcinogenesis and lung genotoxicity. While genetic factors involved in the susceptibility to MPM have been reported, to date the impact of individual genetic variations on asbestos-related lung cancer risk is still poorly understood. Since inflammation and disruption of iron homeostasis are hallmarks of asbestos exposure affecting the lung tissue, in this study we aimed at investigating the association between iron-metabolism and inflammasome gene variants and susceptibility to develop LC, by comparing an asbestos exposed population affected by LC with an "asbestos-resistant exposed population". We employed a retrospective approach same as our previous autopsy-based pilot study and we also replicated our previous findings on MPM by repeating the analysis in a novel cohort of autoptic samples. We confirmed the protective role of HEPH coding SNP but also of the two non-coding SNP, either in FTH1 or in TF, in a novel cohort of mesothelioma exposed individuals and we found the same protective genetic variants in a cohort of LC exposed individuals, from the same geographic area of MPM subjects, suggesting a common "protection profile". No association was found between NLRP1 and NLRP3 polymorphisms with susceptibility to develop MPM and LC. Further research into a specific MPM and LC "genetic signature" would be needed to broaden our knowledge of the genetic landscape causative of MPM and LC.

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Synchrotron X-ray microscopy reveals early calcium and iron interaction with crocidolite fibers in the lung of exposed mice

Cited by 15 publications

References 48 publications

Iron-related toxicity of single-walled carbon nanotubes and crocidolite fibres in human mesothelial cells investigated by Synchrotron XRF microscopy

Iron-related toxicity of single-walled carbon nanotubes and crocidolite fibres in human mesothelial cells investigated by Synchrotron XRF microscopy

Therapeutic Potential of Small Molecules Targeting Oxidative Stress in the Treatment of Chronic Obstructive Pulmonary Disease (COPD): A Comprehensive Review

Pleural mesothelioma and lung cancer: the role of asbestos exposure and genetic variants in selected iron metabolism and inflammation genes

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