Synchrotron FTIR and Raman spectroscopy 1 ectroscopy provide unique spectral fingerprints for <i>Arabidopsis</i> floral stem vascular tissues

Dinant, Sylvie; Wolff, Nelly; Marco, Federica De; Vilaine, Françoise; Gissot, Lionel; Aubry, Emilie; Sandt, Christophe; Bellini, Catherine; Hir, Rozenn Le

doi:10.1101/343343

Cited by 2 publications

(1 citation statement)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Analysis of mRNA levels of the genes of pectin synthesis [UDP-Dglucuronate 4-epimerase (GEA), galacturonate transferase 1 (GAUT1), galacturonate transferase 7 (GAUT7), xylose:rhamnogalacturonate (Popescu et al, 2007;Cheng et al, 2016;Javier-Astete et al, 2021) 1372 Cellulose and hemicellulose: CH bending, C-H deformation vibration (Popescu et al, 2007;Szymanska-Chargot and Zdunek, 2013;Largo-Gosens et al, 2014;Cheng et al, 2016;Chow and Ting, 2019;Dinant et al, 2019;Javier-Astete et al, 2021) 1343 Cellulose: CH bending Lignin: C 1 -O vibrations in syringyl derivatives (Popescu et al, 2007;Cheng et al, 2016) 1319 Cellulose: CH2 wagging (Popescu et al, 2007;Szymanska-Chargot and Zdunek, 2013;Largo-Gosens et al, 2014;Cheng et al, 2016;Javier-Astete et al, 2021) 1260 Lignin: Guaiacyl ring breathing, C-O linkage in guaiacyl aromatic methoxyl groups (Popescu et al, 2007;Largo-Gosens et al, 2014;Cheng et al, 2016;Dinant et al, 2019) 1230 Lignin: syringyl ring breathing, Hemicellulose, pectin: C-O stretching (Popescu et al, 2007;Szymanska-Chargot and Zdunek, 2013;Lahlali et al, 2017;Dinant et al, 2019) 1150 Cellulose, hemicellulose, pectin: C-O-C asymmetric stretching (Popescu et al, 2007;Szymanska-Chargot and Zdunek, 2013;Largo-Gosens et al, 2014;Escudero et al, 2017;Lahlali et al, 2017;Dinant et al, 2019;Javier-Astete et al, 2021) transferase II (RGXT), arabinose transferase (ARAD), pectin methyltransferase (PMT)] and degradation (pectin methylesterase 1 (PME1), pectin methylesterase 3 (PME3), pectin methylesterase 5 (PME5), polygal...…”

Section: Transcript Levels Of Pectin Metabolism Genes In Flax Seedlin...mentioning

confidence: 99%

Endophytic non-pathogenic Fusarium oxysporum reorganizes the cell wall in flax seedlings

Wojtasik,

Dymińska,

Hanuza

et al. 2024

Front. Plant Sci.

View full text Add to dashboard Cite

IntroductionFlax (Linum usitatissimum) is a crop producing valuable products like seeds and fiber. However, its cultivation faces challenges from environmental stress factors and significant yield losses due to fungal infections. The major threat is Fusarium oxysporum f.sp lini, causing fusarium wilt of flax. Interestingly, within the Fusarium family, there are non-pathogenic strains known as biocontrols, which protect plants from infections caused by pathogenic strains. When exposed to a non-pathogenic strain, flax exhibits defense responses similar to those seen during pathogenic infections. This sensitization process activates immune reactions, preparing the plant to better combat potential pathogenic strains. The plant cell wall is crucial for defending against pathogens. It serves as the primary barrier, blocking pathogen entry into plant cellsMethodsThe aim of the study was to investigate the effects of treating flax with a non-pathogenic Fusarium oxysporum strain, focusing on cell wall remodeling. The infection’s progress was monitored by determining the fungal DNA content and microscopic observation. The plant defense response was confirmed by an increase in the level of Pathogenesis-Related (PR) genes transcripts. The reorganization of flax cell wall during non-pathogenic Fusarium oxysporum strain infection was examined using Infrared spectroscopy (IR), determination of cell wall polymer content, and analysis of mRNA level of genes involved in their metabolism.Results and discussionIR analysis revealed reduced cellulose content in flax seedlings after treatment with Fo47 and that the cellulose chains were shorter and more loosely bound. Hemicellulose content was also reduced but only after 12h and 36h. The total pectin content remained unchanged, while the relative share of simple sugars and uronic acids in the pectin fractions changed over time. In addition, a dynamic change in the level of methylesterification of carboxyl groups of pectin was observed in flax seedlings treated with Fo47 compared to untreated seedlings. The increase in lignin content was observed only 48 hours after the treatment with non-pathogenic Fusarium oxysporum. Analysis of mRNA levels of cell wall polymer metabolism genes showed significant changes over time in all analyzed genes. In conclusion, the research suggests that the rearrangement of the cell wall is likely one of the mechanisms behind flax sensitization by the non-pathogenic Fusarium oxysporum strain. Understanding these processes could help in developing strategies to enhance flax’s resistance to fusarium wilt and improve its overall yield and quality.

show abstract

Section: Transcript Levels Of Pectin Metabolism Genes In Flax Seedlin...mentioning

confidence: 99%

Endophytic non-pathogenic Fusarium oxysporum reorganizes the cell wall in flax seedlings

Wojtasik,

Dymińska,

Hanuza

et al. 2024

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

Lateral Transport of Organic and Inorganic Solutes

Aubry

Dinant

Vilaine

et al. 2019

Plants

View full text Add to dashboard Cite

Organic (e.g., sugars and amino acids) and inorganic (e.g., K+, Na+, PO42−, and SO42−) solutes are transported long-distance throughout plants. Lateral movement of these compounds between the xylem and the phloem, and vice versa, has also been reported in several plant species since the 1930s, and is believed to be important in the overall resource allocation. Studies of Arabidopsis thaliana have provided us with a better knowledge of the anatomical framework in which the lateral transport takes place, and have highlighted the role of specialized vascular and perivascular cells as an interface for solute exchanges. Important breakthroughs have also been made, mainly in Arabidopsis, in identifying some of the proteins involved in the cell-to-cell translocation of solutes, most notably a range of plasma membrane transporters that act in different cell types. Finally, in the future, state-of-art imaging techniques should help to better characterize the lateral transport of these compounds on a cellular level. This review brings the lateral transport of sugars and inorganic solutes back into focus and highlights its importance in terms of our overall understanding of plant resource allocation.

show abstract

Synchrotron FTIR and Raman spectroscopy 1 ectroscopy provide unique spectral fingerprints for Arabidopsis floral stem vascular tissues

Cited by 2 publications

References 63 publications

Endophytic non-pathogenic Fusarium oxysporum reorganizes the cell wall in flax seedlings

Endophytic non-pathogenic Fusarium oxysporum reorganizes the cell wall in flax seedlings

Lateral Transport of Organic and Inorganic Solutes

Contact Info

Product

Resources

About