Synchrotron fourier transform infrared microspectroscopy: A new tool to monitor the fate of organic contaminants in plants

Dokken, K.; Davis, Lawrence C.; Erickson, Larry E.; Castro-Diaz, Sigifredo; Marinković, Nebojša

doi:10.1016/j.microc.2005.01.022

Cited by 28 publications

(14 citation statements)

References 18 publications

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“…The transpiration stream concentration factor, which is the ratio of the concentrations in the xylem and solution, was greater than 1.5 for all compounds except for hydroxybenzotriazole indicating translocation into the xylem of the plant. Interestingly, Dokken et al (2005) used Fourier transformed infrared (FTIR) spectroscopy to study the uptake of benzotriazole by sunflower plants. The FTIR spectra of the roots showed peaks for changes in the lignin structure and the presence of benzotriazole.…”

Section: Studies On the Treatment Of Eocsmentioning

confidence: 99%

Literature overview: Emerging organic contaminants in water and their remediation

Husain

2008

Remediation Journal

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Section: Studies On the Treatment Of Eocsmentioning

confidence: 99%

Literature overview: Emerging organic contaminants in water and their remediation

Husain

2008

Remediation Journal

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“…This technique has emerged as a key technique for studying tissue cell-wall components (McCann & Roberts, 1994;McCann et al, 1995McCann et al, , 1997Wetzel et al, 1998;Yu et al, 2004;Dokken et al, 2007), for studying plant growth and development (Dokken et al, 2007;McCann & Roberts, 1994;McCann et al, 1995McCann et al, , 1997, monitoring the fate of organic contaminants in plants (Dokken et al, 2005) and studying the bread-making quality of wheat (Bonwell et al, 2008). Dokken (2006) indicated that current wet chemistry techniques, although helpful, degrade plant tissue resulting in the loss of spatial distribution and the production of artifacts because wet chemistry techniques employ strong chemical processing and extractions such as GC-MS (gas chromatography mass spectrometry) and HPLC (high-performance liquid chromatography). SR-IMS permits direct analysis of plant cell-wall architecture, combining spatially localized information and chemical information from the infrared absorbance to produce a chemical map that can be linked to a particular morphology or functional group (Budevska, 2002;Dokken, 2006;Dokken et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

“…Dokken (2006) indicated that current wet chemistry techniques, although helpful, degrade plant tissue resulting in the loss of spatial distribution and the production of artifacts because wet chemistry techniques employ strong chemical processing and extractions such as GC-MS (gas chromatography mass spectrometry) and HPLC (high-performance liquid chromatography). SR-IMS permits direct analysis of plant cell-wall architecture, combining spatially localized information and chemical information from the infrared absorbance to produce a chemical map that can be linked to a particular morphology or functional group (Budevska, 2002;Dokken, 2006;Dokken et al, 2007). Dokken (2006) showed that SR-IMS, as an important analytical tool, could be used to determine the fate and effect of organic contaminants in plants.…”

Section: Introductionmentioning

confidence: 99%

“…SR-IMS permits direct analysis of plant cell-wall architecture, combining spatially localized information and chemical information from the infrared absorbance to produce a chemical map that can be linked to a particular morphology or functional group (Budevska, 2002;Dokken, 2006;Dokken et al, 2007). Dokken (2006) showed that SR-IMS, as an important analytical tool, could be used to determine the fate and effect of organic contaminants in plants.…”

Section: Introductionmentioning

confidence: 99%

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Microprobing the molecular spatial distribution and structural architecture of feed-type sorghum seed tissue (Sorghum Bicolor L.) using the synchrotron radiation infrared microspectroscopy technique

2011

J Synchrotron Radiat

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Sorghum seed (Sorghum bicolor L.) has unique degradation and fermentation behaviours compared with other cereal grains such as wheat, barley and corn. This may be related to its cell and cell-wall architecture. The advanced synchrotron radiation infrared microspectroscopy (SR-IMS) technique enables the study of cell or living cell biochemistry within cellular dimensions. The objective of this study was to use the SR-IMS imaging technique to microprobe molecular spatial distribution and cell architecture of the sorghum seed tissue comprehensively. High-density mapping was carried out using SR-IMS on beamline U2B at the National Synchrotron Light Source (Brookhaven National Laboratory, NY, USA). Molecular images were systematically recorded from the outside to the inside of the seed tissue under various chemical functional groups and their ratios [peaks at $ 1725 (carbonyl C O ester), 1650 (amide I), 1657 (protein secondary structure-helix), 1628 (protein secondary structuresheet), 1550 (amide II), 1515 (aromatic compounds of lignin), 1428, 1371, 1245 (cellulosic compounds in plant seed tissue), 1025 (non-structural CHO, starch granules), 1246 (cellulosic material), 1160 (CHO), 1150 (CHO), 1080 (CHO), 930 (CHO), 860 (CHO), 3350 (OH and NH stretching), 2960 (CH 3 antisymmetric), 2929 (CH 2 anti-symmetric), 2877 (CH 3 symmetric) and 2848 cm À1 (CH 2 asymmetric)]. The relative protein secondary structure-helix to-sheet ratio image, protein amide I to starch granule ratio image, and anti-symmetric CH 3 to CH 2 ratio image were also investigated within the intact sorghum seed tissue. The results showed unique cell architecture, and the molecular spatial distribution and intensity in the sorghum seed tissue (which were analyzed through microprobe molecular imaging) were generated using SR-IMS. This imaging technique and methodology has high potential and could be used for scientists to develop specific cereal grain varieties with targeted food and feed quality, and can also be used to monitor the degree of grain maturity, grain damage, the fate of organic contaminants and the effect of chemical treatment on plant and grain seeds.

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“…The imaging ability of infrared microspectroscopy (IMS) permits the analysis of plant cell wall architecture at the cellular level. The use of synchrotron radiation as an infrared source and the application of chemometric methods, such as principle components analysis (PCA), make IMS a powerful tool for the study of plant growth and development (Dokken et al, 2005a,b,c).…”

mentioning

confidence: 99%

Infrared Monitoring of Dinitrotoluenes in Sunflower and Maize Roots

Dokken

Davis

2011

J. environ. qual.

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Infrared microspectroscopy (IMS) is emerging as an important analytical tool for the structural analysis of biological tissue. Th is report describes the use of IMS coupled to a synchrotron source combined with principal components analysis (PCA) to monitor the fate and eff ect of dinitrotoluenes in the roots of maize and sunfl ower plants. Infrared imaging revealed that maize roots metabolized 2,4-dinitrotoluene (DNT) and 2,6-DNT. Th e DNTs and their derivative aromatic amines were predominantly associated with epidermis and xylem. Both isomers of DNT altered the structure and production of pectin and pectic polysaccharides in maize and sunfl ower plant roots. Infrared peaks diagnostic for aromatic amines were seen at the 5 mg L −1 concentrations for both DNTs in maize and sunfl ower treated tissue. However, only infrared peaks for nitro groups, not aromatic amines, were present in the maize treated at 10 mg L −1 . For sunfl ower, the 10 mg L −1 level was toxic and also produced very dark root systems making spectra diffi cult to obtain. Maize and sunfl ower seem unable to metabolize eff ectively at concentrations higher than about 5 mg L −1 DNT in hydroponic solution. Based on the results of this study, IMS combined with PCA can be an eff ective means of determining the fate and metabolism of organic contaminants in plant tissue when isotopically labeled compounds are not available.

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Synchrotron fourier transform infrared microspectroscopy: A new tool to monitor the fate of organic contaminants in plants

Cited by 28 publications

References 18 publications

Literature overview: Emerging organic contaminants in water and their remediation

Literature overview: Emerging organic contaminants in water and their remediation

Microprobing the molecular spatial distribution and structural architecture of feed-type sorghum seed tissue (Sorghum Bicolor L.) using the synchrotron radiation infrared microspectroscopy technique

Infrared Monitoring of Dinitrotoluenes in Sunflower and Maize Roots

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