Synchronous optical and electrical detection of biomolecules traversing through solid-state nanopores

Soni, Gautam V.; Singer, Alexander; Yu, Zhiliang; Sun, Yingjie; McNally, Ben; Meller, A.

doi:10.1063/1.3277116

Cited by 92 publications

(103 citation statements)

References 30 publications

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“…Aside from electrical detection, optical fluorescence detection has also been used to successfully monitor translocation events using membranes made from SiN x or a combination of SiN x and aluminum 9,16,17 .…”

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Rapid Ultrasensitive Single Particle Surface-Enhanced Raman Spectroscopy Using Metallic Nanopores

et al. 2013

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Nanopore sensors embedded within thin dielectric membranes have been gaining significant interest due to their single molecule sensitivity and compatibility of detecting a large range of analytes, from DNA and proteins, to small molecules and particles. Building on this concept we utilize a metallic Au solidstate membrane to translocate and rapidly detect single Au nanoparticles (NPs) functionalized with 589 dye molecules using surface enhanced resonance Raman Spectroscopy (SERRS). We show that due to the plasmonic coupling between the Au metallic nanopore surface and the NP, signal intensities are enhanced when probing analyte molecules bound to the NP surface. Although not single molecule, this nanopore sensing scheme benefits from the ability of SERS to provide rich vibrational information on the analyte, improving on current nanopore-based electrical and optical detection techniques. We show that the full vibrational band of the analyte can be detected with ultra-high spectral sensitivity, and rapid temporal resolution of 880 µs.

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“…There have also been examples of combining both electrical and optical detection to maximize the information that can be extracted from each and every single molecule translocating through the nanopore 17 .…”

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Rapid Ultrasensitive Single Particle Surface-Enhanced Raman Spectroscopy Using Metallic Nanopores

et al. 2013

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“…21,22 Among many techniques, fluorescent imaging is well established in studying the behavior of molecules in a variety of confined spaces. 23 By combining ionic current signal with molecule fluorescence imaging, we will directly observe whether the decrease in ionic current does in fact correspond to the blockade of the channels by ambient stimuli.…”

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confidence: 99%

Coordination of the electrical and optical signals revealing nanochannels with an ‘onion-like’ gating mechanism and its sensing application

Zhao

Gao

et al. 2016

NPG Asia Mater

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Artificial stimuli-responsive nanochannels that mimic the gating property of biological ion channels to control the movement of ions across the membranes have attracted much attention. However, the ionic current is also greatly influenced by many environmental factors in the reaction system. In the present study, we coordinated the ionic current and the fluorescence signal to reveal that the gatekeepers (here, oligomers) open and close the nanochannels with an 'onion-like' intermediate state, as confirmed by molecular dynamics simulations. The dual-signal-output nanochannels exhibited a high sensitivity and selectivity to glucose and showed a high antijamming property with regard to impurities such as ascorbic acid (Vc) and H 2 O 2 in complicated practical environments, which could induce false signals in traditional glucose detection methods. Ten healthy individuals' urine samples were distinguished from those of 15 diabetes patients; moreover, the urine samples of the diabetes patients could be discriminated before and after treatment with insulin.

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“…10 In addition, the nanopore platform provides the potential to enhance optical detection via control of throughput, incorporation of additional photonic structure or use as a zero-mode waveguide. 7,11 A number of optical configurations have so far been reported, these include the use of wide-field imaging 7 , liquid core anti-resonant reflecting optical waveguides 12 , total internal reflection fluorescence microscopy 13,14 and confocal fluorescence microscopy. 7 For structural information to be probed via resistive pulse sensing, high temporal resolution measurements are crucial due to the high translocation velocity of molecules.…”

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Synchronized Optical and Electronic Detection of Biomolecules Using a Low Noise Nanopore Platform

et al. 2015

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In the past two decades there has been a tremendous amount of research into the use of nanopores as single molecule sensors, which has been inspired by the Coulter counter and molecular transport across biological pores. Recently, the desire to increase structural resolution and analytical throughput has led to the integration of additional detection methods such as fluorescence spectroscopy. For structural information to be probed electronically high bandwidth measurements are crucial due to the high translocation velocity of molecules. The most commonly used solid-state nanopore sensors consist of a silicon nitride membrane and bulk silicon substrate. Unfortunately, the photoinduced noise associated with illumination of these platforms limits their applicability to high-bandwidth, high-laser-power synchronized optical and electronic measurements. Here we present a unique low-noise nanopore platform, composed of a predominately Pyrex substrate and silicon nitride membrane, for synchronized optical and electronic detection of biomolecules. Proof of principle experiments are conducted showing that the Pyrex substrates have substantially lowers ionic current noise arising from both laser illumination and platform capacitance. Furthermore, using confocal microscopy and a partially metallic pore we demonstrate high signal-to-noise synchronized optical and electronic detection of dsDNA.

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Synchronous optical and electrical detection of biomolecules traversing through solid-state nanopores

Cited by 92 publications

References 30 publications

Rapid Ultrasensitive Single Particle Surface-Enhanced Raman Spectroscopy Using Metallic Nanopores

Rapid Ultrasensitive Single Particle Surface-Enhanced Raman Spectroscopy Using Metallic Nanopores

Coordination of the electrical and optical signals revealing nanochannels with an ‘onion-like’ gating mechanism and its sensing application

Synchronized Optical and Electronic Detection of Biomolecules Using a Low Noise Nanopore Platform

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