Synchronous germination of Streptomyces antibioticus spores: Tool for the analysis of hyphal growth in liquid cultures

Miguélez, Elisa M.; Martín, Cruz; Hardisson, Carlos; Manzanal, Manuel B.

doi:10.1111/j.1574-6968.1993.tb06156.x

Cited by 16 publications

(1 citation statement)

References 10 publications

(11 reference statements)

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“…Although heat shock 57 and ultrasonic treatments 58 may increase the germination rates of streptomycetes, very little is known about the germination mechanism. We found that the 12 MHz sine-wave potential induced the maximum number of CFUs in the attached ST.28 spores and increased the germination rate by 2.8 fold (Fig.…”

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Electrical Retrieval of Living Streptomycete Spores Using a Potential-Controlled ITO Electrode

et al. 2017

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The purpose of this study was to develop a novel electrical retrieval method for living spores of streptomycetes. Five strains of deep-sea Streptomyces sp. and typical strain Streptomyces albus suspended in 1/20 artificial seawater (1/20ASW) were selectively attached to an indium tin oxide/glass (ITO) working electrode region to which a −0.2 V vs. Ag/AgCl constant potential was applied for 24 h. Spores of all six streptomycetes produced either short fibrous or membranous materials and attached to the ITO electrode region. A +20 mV vs. Ag/AgCl, 12 MHz sine-wave potential was further applied for another 1 h to the deep-sea Streptomyces sp. ST.28 spores on the ITO electrode and colony-forming units increased 3.5 fold after 3 day cultivation compared with controls. The ST.28 spores almost completely lost the ability to adhere to the potential-applied ITO electrode when dispersed in Mg 2+ free 1/20ASW. We succeeded in the attachment and cultivation of specifically positioned single ST.28 spores on an ITO microelectrode surface with −0.2 V vs. Ag/AgCl potential application. A combination of micropatterning techniques for a living single streptomycete spore on the ITO electrode and omics technologies holds potential for new bioactive compound screening concepts and applications.

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Electrical Retrieval of Living Streptomycete Spores Using a Potential-Controlled ITO Electrode

et al. 2017

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Cultivation and Diversity of Marine Actinomycetes: Molecular Approaches and Bioinformatics Tools

et al. 2022

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Constitution of the metabolic type of streptomycetes during the first hours of cultivation

et al. 1997

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Using the examples of biosynthesis of streptomycin, bialaphos, actinorhodin, oligoketides and autoregulators during the first hours of streptomycete cultivation, it is stressed that the external environment in cooperation with the internal metabolic abilities of the cell determines the metabolic type that would develop during the life cycle of the producing streptomycetes. If we accept that a certain metabolic type (from the point of view of the production of secondary metabolites) was determined already during the first hours of cultivation of the microorganisms, we must also admit that the availability of primary metabolites in the so-called production phase of growth (stationary phase, idiophase, etc.) is to a certain extent determined by the very early stages of strain development.

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Synchronous germination of Streptomyces antibioticus spores: Tool for the analysis of hyphal growth in liquid cultures

Cited by 16 publications

References 10 publications

Electrical Retrieval of Living Streptomycete Spores Using a Potential-Controlled ITO Electrode

Electrical Retrieval of Living Streptomycete Spores Using a Potential-Controlled ITO Electrode

Cultivation and Diversity of Marine Actinomycetes: Molecular Approaches and Bioinformatics Tools

Constitution of the metabolic type of streptomycetes during the first hours of cultivation

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