Synchronized research on endothelial dysfunction and microcirculation structure in dorsal skin of rats with type 2 diabetes mellitus

Wei, Yajie; Chen, Huimin; Chi, Qingzhuo; He, Ying; Mu, Lizhong; Liu, Chao; Lu, Yunxia

doi:10.1007/s11517-021-02363-5

Cited by 7 publications

(16 citation statements)

References 32 publications

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“…Despite of the reasonable structure and sizes of the vessels, it should be noted that the model has not reflected the individual differences in the foot structure. Our rat experimental study has shown that the microvasculature alteration occurs after the endothelial dysfunction (Wei et al, 2021). In the current study, it is assumed that the microvasculature has not been changed since the blood flow alterations due to the change of vasodilation is the first aim of the work.…”

Section: Discussionmentioning

confidence: 95%

“…Additionally, the medical image could only reflect the structural lesions but not functional lesions. It is found that the diabetic vascular functional lesions may appear before structural lesions through rat experiments (Wei et al, 2021). Coupling with thermography and the porous media model, the stenosis degree of peripheral vessels, vascular density variation, or vasodilation dysfunction may be detected, which are meaningful for the early diagnosis of a diabetic foot.…”

Section: Discussionmentioning

confidence: 95%

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Thermal Analysis of Blood Flow Alterations in Human Hand and Foot Based on Vascular-Porous Media Model

Wang

Cheng

et al. 2022

Front. Bioeng. Biotechnol.

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Microvascular and Macrovascular diseases are serious complications of diabetic mellitus, which significantly affect the life quality of diabetic patients. Quantitative description of the relationship between temperature and blood flow is considerably important for non-invasive detection of blood vessel structural and functional lesions. In this study, thermal analysis has been employed to predict blood flow alterations in a foot and a cubic skin model successively by using a discrete vessel-porous media model and further compared the blood flows in 31 diabetic patients. The tissue is regarded as porous media whose liquid phase represents the blood flow in capillaries and solid phase refers to the tissue part. Discrete vascular segments composed of arteries, arterioles, veins, and venules were embedded in the foot model. In the foot thermal analysis, the temperature distributions with different inlet vascular stenosis were simulated. The local temperature area sensitive to the reduction of perfusion was obtained under different inlet blood flow conditions. The discrete vascular-porous media model was further applied in the assessment of the skin blood flow by coupling the measured skin temperatures of diabetic patients and an inverse method. In comparison with the estimated blood flows among the diabetic patients, delayed blood flow regulation was found in some of diabetic patients, implying that there may be some vascular disorders in these patients. The conclusion confirms the one in our previous experiment on diabetic rats. Most of the patients predicted to be with vascular disorders were diagnosed as vascular complication in clinical settings as well, suggesting the potential applications of the vascular-porous media model in health management of diabetic patients.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 95%

Thermal Analysis of Blood Flow Alterations in Human Hand and Foot Based on Vascular-Porous Media Model

Wang

Cheng

et al. 2022

Front. Bioeng. Biotechnol.

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“…Effects of heating test on AFC of SBP oscillation in neurogenic and myogenic intervals differ in mice and rats (Wei et al, 2021). It may be explained by differences of experimental conditions.…”

Section: Discussionmentioning

confidence: 96%

“…A widely used provocative test is local heating of measuring place to 38‐42 ℃ (Minson, 2010). The effect of local heating on skin microcirculation has been well studied in humans and rats (Brunt et al, 2011; Geyer et al, 2004; A. V. Tankanag et al, 2017; Wei et al, 2021), but there were no studies on mice. The effects of heat test on [Ca 2+ ] i and [NO] i oscillations at the cellular level also require investigation.…”

Section: Introductionmentioning

confidence: 99%

“…Models on laboratory animals are of great interest, because they may allow studying the relationship of SBP oscillations with processes at the cellular level. Laboratory animals also allow research effects of socially significant diseases on SBP oscillations by methods, which are not applicable in research on humans (Wei et al, 2021). Previously, we adapted the method of SBP registration by laser Doppler flowmetry (LDF) for studies on mice (Astashev et al, 2019) which made it possible to research the effects of systemic induced hypoxia on the AFC of SBP oscillations.…”

Section: Introductionmentioning

confidence: 99%

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Low‐frequency oscillations of murine skin microcirculations and periodic changes of [Ca²⁺]_i and [NO]_i levels in murine endotheliocytes: An effect of provocative tests

Serov

Tankanag

Astashev

2021

Cell Biology International

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The five frequency intervals of skin blood oscillation were described: cardiac, respiratory, myogenic, neurogenic, and endothelial. The endothelial interval is derived into NO‐independent and NO‐dependent. The exact molecular, cell, or systemic mechanisms of endothelial oscillations generation are unclear. We proposed that oscillations of Ca2+ and NO in endotheliocytes may be possible sources of skin blood perfusion (SBP) oscillations in endothelial interval. To examine our hypothesis we compared the oscillations of cytoplasmic Ca2+ and NO ([Ca2+]i and [NO]i) concentration in cultured murine microvascular endotheliocytes and SBP oscillations in mice. Local heating test and model hypoxia were used as tools to evaluate an interconnection of studied parameters. [Ca2+]i and [NO]i were measured simultaneously by Fura‐2 AM and DAF‐FM. The SBP was measured by laser Doppler flowmetry. The [Ca2+]i and [NO]i oscillations at 0.005−0.01 Hz were observed in endotheliocytes, that coincides the ranges of NO‐independent endothelial interval. Heating decreased amplitude of [Ca2+]i and [NO]i oscillations in cells in NO‐independent endothelial interval, while amplitudes of SBP oscillations increased in NO‐independent and NO‐dependent intervals. Hypoxia reduced the [NO]i oscillations amplitude. Heating test during hypoxia increased NO‐independent endothelial SBP oscillations and decreased myogenic ones, did not effect on [NO]i oscillations, and shifted [Ca2+]i oscillations peak from 0.005−0.01 Hz to 0.01−0.018 Hz. We observed the [Ca2+]i and [NO]i oscillations synchronization within a cell and between cells for the first time. Heating abolished these synchronizations. Therefore low‐frequency [Ca2+]i and [NO]i oscillations in endotheliocytes may be considered as modulators of low‐frequency endothelial SBP oscillations.

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NO transport characteristics in microcirculation and its implications in the assessment of microvascular dysfunction in type 2 diabetes mellitus

He,

Liu,

Long

et al. 2024

Medicine in Novel Technology and Devices

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Synchronized research on endothelial dysfunction and microcirculation structure in dorsal skin of rats with type 2 diabetes mellitus

Cited by 7 publications

References 32 publications

Thermal Analysis of Blood Flow Alterations in Human Hand and Foot Based on Vascular-Porous Media Model

Thermal Analysis of Blood Flow Alterations in Human Hand and Foot Based on Vascular-Porous Media Model

Low‐frequency oscillations of murine skin microcirculations and periodic changes of [Ca²⁺]_i and [NO]_i levels in murine endotheliocytes: An effect of provocative tests

NO transport characteristics in microcirculation and its implications in the assessment of microvascular dysfunction in type 2 diabetes mellitus

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Synchronized research on endothelial dysfunction and microcirculation structure in dorsal skin of rats with type 2 diabetes mellitus

Cited by 7 publications

References 32 publications

Thermal Analysis of Blood Flow Alterations in Human Hand and Foot Based on Vascular-Porous Media Model

Thermal Analysis of Blood Flow Alterations in Human Hand and Foot Based on Vascular-Porous Media Model

Low‐frequency oscillations of murine skin microcirculations and periodic changes of [Ca2+]i and [NO]i levels in murine endotheliocytes: An effect of provocative tests

NO transport characteristics in microcirculation and its implications in the assessment of microvascular dysfunction in type 2 diabetes mellitus

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Low‐frequency oscillations of murine skin microcirculations and periodic changes of [Ca²⁺]_i and [NO]_i levels in murine endotheliocytes: An effect of provocative tests