Synchronized Excitation of Fluorescence Emission Spectra

Lloyd, John

doi:10.1038/physci231064a0

Cited by 462 publications

(214 citation statements)

References 1 publication

Supporting

Mentioning

208

Contrasting

Unclassified

Order By: Relevance

“…Synchronous fluorescence spectroscopy. Synchronous fluorescence spectroscopy is an instrumental technique involving the simultaneous scanning of the excitation and emission monochromators, keeping a constant wavelength or energy offset between the monochromators (Lloyd 1971, Vo-Dinh 1978, Inman & Winefordner 1982. This technique allows a better resolution of individual fluorescent components of DOM through a narrowing of the emission band spectra of a single fluorescent structure.…”

Section: Methodsmentioning

confidence: 99%

Synchronous fluorescence spectra of dissolved organic matter (DOM) of algal origin in marine coastal waters

Gm¹,

Mingazzini²

1995

Mar. Ecol. Prog. Ser.

View full text Add to dashboard Cite

ABSTRACT-Naturally occurring dissolved organic matter [DOM) was investigated in a lagoonal system with the aim to study the origin and mixing of the Doh4 components. Water samples were collected in the Sacca di Goro lagoon (PO river delta, Italy) and analysed by means of different techniques, such as fluorescence, absorpt~on and dissolved organic carbon (DOC) measurements. Synchronous fluorescence spectra obtained from both laboratory algal cultures and benthic macroalgae collected in situ were compared with spectra from field samples in order to distinguish DOM components having autochthonous (algal) origin with respect to remote formation-Two main components could be identified on the basis of their synchronous fluorescent bands. The results indicated that the first band (band hA at h = 275 nm), prevailing in seawater samples, could reflect a recent biological production, while the second (bands hB and hC at h > 300 nm) prevailing in the tributaries water samples, could likely be due to older or remote formation. The synchronous peak height ratio hA:hB is proposed as an useful parameter describing the different contribution and mixing of the DOM components present in the lagoonal-estuarine waters.

show abstract

Section: Methodsmentioning

confidence: 99%

Synchronous fluorescence spectra of dissolved organic matter (DOM) of algal origin in marine coastal waters

Gm¹,

Mingazzini²

1995

Mar. Ecol. Prog. Ser.

View full text Add to dashboard Cite

show abstract

“…Alternatively, multicomponent fluorescent systems may be investigated by the synchronous fluorescence techniques, proposed by Lloyd, (1971). This technique involves simultaneous scanning of both excitation and emission wavelengths, keeping a constant difference between them.…”

Section: Fluorescence Characteristics Of Olive Oil and Its Componentsmentioning

confidence: 99%

Analysis of Olive Oils by Fluorescence Spectroscopy: Methods and Applications

Sikorska¹,

Khmelinskii²,

Sikorski³

2012

Olive Oil - Constituents, Quality, Health Properties and Bioconversions

View full text Add to dashboard Cite

“…Compared with general fluorescence, the synchronous fluorescence spectra (SFS) was a multidimensional fluorescence technique which involves in scanning simultaneously by the excitation and emission monochromators while maintaining a constant wavelength interval (Dl) between l ex and l em [23]. This technique could not only maintain the sensitivity, but also simplify the emission spectra, improve the selectivity and spectral resolution, decrease the interference due to light scattering [24,25].…”

Section: Introductionmentioning

confidence: 99%