Synchronization of porcine fetal fibroblast cells with topoisomerase-inhibitor hoechst 33342

Effects of high-dose major components in oral disinfectants on the cell cycle and apoptosis in primary human gingival fibroblasts in vitro

“…To synchronize the cells in the G0/G1 phase, we used the method of serum deprivation 22) by incubating HGFs in DMEM with only 0.5% FBS for 48 h. To induce the cells to re-enter the cell cycle, we incubated the HGFs for an additional 24 h in fresh DMEM culture medium containing 10% FBS and the IC50 (BEC 1.3×10 -2 mM, BAC 6.0×10 -3 mM, PVD-I 2.6×10 -1 mM) of the three disinfectants that were used for the cell growth assay described above. Both the floating and attached cells were collected using trypsin-EDTA, resuspended in DMEM without FBS, washed with PBS, and fixed in a solution of 70% ice-cold ethanol at -20ºC overnight.…”

Influence of chlorine dioxide on cell death and cell cycle of human gingival fibroblasts

“…21 We induced the cells to re-enter the cell cycle by incubation in fresh medium with the disinfectants at the final concentration (determined from the growth assay results) for a further 24 h. The cellular DNA content was determined by flow cytometry, as described previously. 22 We collected the floating and attached cells using trypsin-EDTA and resuspended them in DMEM (no FBS).…”

Apototic and Necrotic Influence of Dental Resin Polymerization Initiators in Human Gingival Fibroblast Cultures

“…To synchronize the cells in G 0 /G 1 phase by serum deprivation 22) , HGF cells were incubated in DMEM with 0.5％ FB+S for 48 hours. To induce the cells to re-enter the cell cycle, they were incubated in fresh medium containing 10％ FBS with different concentrations of the four polymerization initiators (final concentrations of 500 μM-5 mM) for another 24 hours.…”