2001
DOI: 10.1016/s0378-4320(01)00088-4
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Synchronization of porcine fetal fibroblast cells with topoisomerase-inhibitor hoechst 33342

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Cited by 11 publications
(10 citation statements)
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“…To synchronize the cells in the G0/G1 phase, we used the method of serum deprivation 22) by incubating HGFs in DMEM with only 0.5% FBS for 48 h. To induce the cells to re-enter the cell cycle, we incubated the HGFs for an additional 24 h in fresh DMEM culture medium containing 10% FBS and the IC50 (BEC 1.3×10 -2 mM, BAC 6.0×10 -3 mM, PVD-I 2.6×10 -1 mM) of the three disinfectants that were used for the cell growth assay described above. Both the floating and attached cells were collected using trypsin-EDTA, resuspended in DMEM without FBS, washed with PBS, and fixed in a solution of 70% ice-cold ethanol at -20ºC overnight.…”
Section: Cell Cycle Analysismentioning
confidence: 99%
“…To synchronize the cells in the G0/G1 phase, we used the method of serum deprivation 22) by incubating HGFs in DMEM with only 0.5% FBS for 48 h. To induce the cells to re-enter the cell cycle, we incubated the HGFs for an additional 24 h in fresh DMEM culture medium containing 10% FBS and the IC50 (BEC 1.3×10 -2 mM, BAC 6.0×10 -3 mM, PVD-I 2.6×10 -1 mM) of the three disinfectants that were used for the cell growth assay described above. Both the floating and attached cells were collected using trypsin-EDTA, resuspended in DMEM without FBS, washed with PBS, and fixed in a solution of 70% ice-cold ethanol at -20ºC overnight.…”
Section: Cell Cycle Analysismentioning
confidence: 99%
“…21 We induced the cells to re-enter the cell cycle by incubation in fresh medium with the disinfectants at the final concentration (determined from the growth assay results) for a further 24 h. The cellular DNA content was determined by flow cytometry, as described previously. 22 We collected the floating and attached cells using trypsin-EDTA and resuspended them in DMEM (no FBS).…”
Section: Cell Cycle Analysismentioning
confidence: 99%
“…To synchronize the cells in G 0 /G 1 phase by serum deprivation 22) , HGF cells were incubated in DMEM with 0.5% FB+S for 48 hours. To induce the cells to re-enter the cell cycle, they were incubated in fresh medium containing 10% FBS with different concentrations of the four polymerization initiators (final concentrations of 500 μM-5 mM) for another 24 hours.…”
Section: Cell Cycle Analysismentioning
confidence: 99%