Synchronization of ovulation in brook char (Salvelinus fontinalis, Mitchill 1814) using emulsified d-Arg6Pro9NEt sGnRHa

Svinger, Viktor W.; Policar, T.; Steinbach, Christoph; Poláková, Simona; Jankovych, Antonin; Kouřil, J.

doi:10.1007/s10499-012-9578-5

Cited by 11 publications

(15 citation statements)

References 65 publications

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“…The increasing demand for salmonid meat, as an aquaculture product with exceptional culinary and health value (Dewailly et al 2007;Wall et al 2010), has led farmers to consider product diversification for better satisfying this demand. Possible alternatives to rainbow trout are brook char and Arctic char, along with their hybrids (Fischer et al 2009;Svinger et al 2013). The potential for rearing primary and complementary species simultaneously in the same facility, for continual production of both species, is being explored.…”

Section: Discussionmentioning

confidence: 99%

“…The increasing demand for rainbow trout has led to an expanded effort to establish new RAS facilities throughout Europe (Terjesen et al 2013), and triggered investigation into diversification of salmonid production. Suggested alternative species are Arctic char Salvelinus alpinus L., brook char Salvelinus fontinalis Mitchill 1814, and their hybrids (Dalsgaard et al 2013;Svinger et al 2013). The feasibility of rearing these species in RAS has been confirmed in past studies (Dalsgaard et al 2013;Buric et al 2015b).…”

Section: Introductionmentioning

confidence: 97%

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Feasibility of rearing brook char fingerlings in an intensive recirculating hatchery as a complementary species to rainbow trout

Buřič¹,

Bláhovec²,

Kouřil³

2016

CZECH J ANIM SCI

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Aquaculture, as the fastest growing agriculture sector, is currently focused on exploring the development of effective intensive recirculating systems (RAS). The use of intensive RAS requires a stable supply of fingerlings throughout the year. Salmonids are a highly important aquaculture species, with rainbow trout Oncorhynchus mykiss often reared in freshwater RASs. The dominant position of rainbow trout has triggered the investigation of a wider diversification of species, including brook char Salvelinus fontinalis. Brook char has the potential to be reared in facilities similar to those used for rainbow trout, but it is not known if brook char is suitable for hatching in an intensive recirculating hatchery system (RHS) to provide a consistent supply of fingerlings to an associated RAS roughly every three months. The present study evaluated the feasibility of producing brook char fingerlings in an RHS and compared results to those obtained with rainbow trout. A production cycle from eyed egg to fingerling was completed separately for rainbow trout, brook char, and parallel rearing of both species for the comparison of growth rate, feed conversion ratio, and the time to reach individual fingerling weight of 2 g. The results showed slower growth rate of brook char compared to rainbow trout reared under the same conditions and a significantly longer production cycle (~108 days), compared to rainbow trout (~74 days). Results suggest that brook char is not suitable for parallel rearing in facilities with primary rainbow trout production. The main practical problem is disruption of the production cycle which requires fingerling stocking at 3-month intervals.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Feasibility of rearing brook char fingerlings in an intensive recirculating hatchery as a complementary species to rainbow trout

Buřič¹,

Bláhovec²,

Kouřil³

2016

CZECH J ANIM SCI

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“…After fertilization and elimination of egg stickiness (Bondarenko et al, 2015), a sample of 200 eggs was collected from each batch. Three egg samples from each female were placed into separate transparent 2.5 l incubators (Svinger et al, 2013c) for incubation and determination of fertilization and hatching rate (Policar et al, 2010). Fertilization rate was determined under a dissecting microscope 3 days post-fertilization when the eggs were at gastrula stage.…”

Section: Methodsmentioning

confidence: 99%

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Bondarenko¹,

Podhorec²,

Svinger³

et al. 2015

Turk. J. Fish. Aquat. Sci.

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Various induction protocols were compared with the aim to reach ovulation in northern pike (Esox lucius L.). The effectiveness of salmon D-Arg 6 Pro 9 NEt-GnRHa at two doses in combination with various additives were tested: 50 and 100 µg kg -1 (groups GnRHa 1 and 2), GnRHa at 50 and 100 µg kg -1 in combination with 8 mg kg -1 metoclopramide (groups 3 and 4), GnRHa at 50 and 100 µg kg -1 emulsified in Freund´s incomplete adjuvant (FIA) (groups 5 and 6), and GnRHa at 50 and 100 µg kg-1 emulsified with FIA 8 mg kg -1 and metoclopramide (groups GnRHa 7 and 8). These treatments were compared to indoor controlled conditions (Group ICC, injected with saline solution, without hormonal treatment) and two traditional treatments: 3 mg kg -1 carp pituitary (Group CP) and ambient outdoor conditions (Group AOC, injected with saline solution only, without hormonal treatment).All fish in Group CP and 70% of those in Group AOC ovulated. The latency periods for Group CP was 96±4 h and 264±58 h for Group AOC. One Group-3 female ovulated spontaneously 108 h post-injection, whereas none of the other GnRHa injected or Group ICC fish ovulated. The pseudogonadosomatic index was 19.3±5.9%; 18.5% and 17.8±7.5% in the AOC, Group 3 and CP, respectively. The fertilization rate reached 88.6±4.5%; 85.5±12.0% and 66.0±13.7% in AOC, Group 3 and CP, respectively. The hatching rate was 68.6±9.9%; 65.5±7.5% and 54.4±8.0% in AOC, Group 3 and CP, respectively.The ovarian fluid pH was significantly higher (8.27±0.03) in AOC compared to CP group (8.11±0.02) (P>0.05). Ovarian fluid of the spontaneously ovulated Group-3 fish had a pH of 8.35. There were no significant differences in egg size and weight among successfully reproduced groups -AOC and CP (P>0.05).

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“…Mylonas et al (1992) reported that fertility and eyeing were reduced when a double injection of only 10 µg/kg BW was used, whereas other authors have found no differences in eggs obtained from treated and untreated females or from females treated with differing doses of GnRHa (Jalabert et al 1978;Donaldson et al 1981;Billard et al 1984;Erdahl and McClain 1987;Slater et al 1995;Arabacı et al 2004;Vazirzadeh et al 2008;Vikingstad et al 2008). Regardless of GnRHa dose, egg viability improved in GnRHa-treated females compared with naturally ovulating female Brook Trout (Svinger et al 2013) and steelhead (anadromous Rainbow Trout; Sower et al 1984).…”

Section: Effects Of Salmon Gonadotropin-releasing Hormone Analog (Gnrmentioning

confidence: 99%

“…An sGnRHa-FIA dose of 25 µg/kg BW and an acute double injection of 25 µg/kg BW were previously found to be highly effective in synchronizing ovulation in Brook Trout (Svinger et al 2013). These doses were selected to lower the costs of treatment.…”

Section: Experimental Designmentioning

confidence: 99%

Effects of Salmon Gonadotropin‐Releasing Hormone Analog (GnRHa) on Reproductive Success and Egg Size in Rainbow Trout and Brook Trout

et al. 2013

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Rainbow Trout Oncorhynchus mykiss were treated with a 100‐ or 25‐μg/kg dose of salmon gonadotropin‐releasing hormone analog (sGnRHa) that was emulsified in Freund's incomplete adjuvant (FIA). Controls were treated with a mixture of FIA and physiological saline. Latency (number of days from the first injection to the date of detected ovulation) was significantly shortened in treated groups compared with the control group. Egg weight, eyeing rate, and hatching rate were significantly reduced in treated groups; mortality after the eyed stage was significantly higher in treated groups. The relationship between latency and the eyeing rate or hatching rate was positive, but the relationship between latency and mortality after the eyed stage was negative. Brook Trout Salvelinus fontinalis were treated with a single injection of sGnRHa–FIA at 12.5 μg/kg, double acute injections of sGnRHa at 12.5 μg/kg administered 3 d apart, or a single acute injection of sGnRHa at 50 μg/kg. Controls were injected with FIA and physiological saline. Latency was significantly shortened in treated groups except the group that received the single acute injection of 50 μg/kg. Egg weight and alevin weight were significantly lower in treated groups. Egg mortality after the eyed stage was significantly higher in the double‐injected group than in the 12.5‐μg/kg sGnRHa–FIA group and the control group. Latency had a negative relationship with the eyeing rate and hatching rate. This result for Brook Trout is opposite that observed for Rainbow Trout. Latency and mortality after the eyed stage were not correlated. It is evident that the experimental designs currently used in salmonid research should be updated to determine reliable means of avoiding egg mortality, particularly in Rainbow Trout, after GnRHa treatment.

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Synchronization of ovulation in brook char (Salvelinus fontinalis, Mitchill 1814) using emulsified d-Arg6Pro9NEt sGnRHa

Cited by 11 publications

References 65 publications

Feasibility of rearing brook char fingerlings in an intensive recirculating hatchery as a complementary species to rainbow trout

Feasibility of rearing brook char fingerlings in an intensive recirculating hatchery as a complementary species to rainbow trout

Untitled

Effects of Salmon Gonadotropin‐Releasing Hormone Analog (GnRHa) on Reproductive Success and Egg Size in Rainbow Trout and Brook Trout

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