Synchronization of cancer cell lines with methotrexate in vitro

Erba, Eugenio; Şen, Sinan

doi:10.1007/bf00122166

Cited by 6 publications

(2 citation statements)

References 27 publications

(13 reference statements)

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“…Such agents include vincristin and vinblastin, both of which are vinca alkaloids from Vinca roseus. Their mechanism of action involves blocking the metaphase in the cell cycle while calcitonin causes significant suppression of cellular proliferation of K562 cells in vitro [14]. The mechanisms of anti-leishmanial and anticancer activities of T. arjuna have not yet been determined but may be similar to those of the aforementioned compounds, respectively.…”

Section: Discussionmentioning

confidence: 99%

Anti-leishmanial and Anti-cancer Activities of a Pentacyclic Triterpenoid Isolated from the Leaves of <i>Terminalia arjuna</i> Combretaceae

Biswas¹,

Kumar²,

Kanti³

2010

Trop. J. Pharm Res

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Purpose: Terminalia arjuna Roxb (Combretaceae) is commonly known as Arjjhan and Arjun in Bengal, India. The anti-leishmanial and anticancer activities of a pentacyclic triterpenoid isolated from its leaves were evaluated. Methods: Dried and crushed leaves of Terminalia arjuna were de-fatted with petroleum ether (40 -60°C) and extracted with methanol. The extract was subjected to column chromatography and column fractions were monitored on TLC plates developed in a suitable solvent system. Structures of the isolated pure compounds were elucidated by infra-red spectroscopy, mass spectrometry and nuclear magnetic resonance spectroscopy. One of the compounds was screened for anti-leishmanial activity against promastigotes of Leishmania donovani, and anti-cancer activity on K562 leukaemic cell line. Results: A pentacyclic triterpenoid, characterized as 3β-hydroxyurs-12-en-28-oic acid (together with ß-D-glucoside) and designated ursolic acid, was successfully isolated. The structure was determined on the basis of spectroscopic analyses. In vitro anti-leishmanial activity against promastigotes of Leishmania donovani (strain AG 83) and anti-cancer activity on K562 leukaemic cell line were shown by the isolated ursolic acid. Conclusion:The methanol extract of the leaves of Terminalia arjuna led to the isolation of a pentacyclic triterpenoid, ursolic acid. This compound demonstrated in vitro anti-leishmanial and anti-cancer activities

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Section: Discussionmentioning

confidence: 99%

Anti-leishmanial and Anti-cancer Activities of a Pentacyclic Triterpenoid Isolated from the Leaves of <i>Terminalia arjuna</i> Combretaceae

Biswas¹,

Kumar²,

Kanti³

2010

Trop. J. Pharm Res

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“…26 However, not all combinations of metabolic blocking agents yield synchronised cell populations. 27 Thus, cell synchronisation techniques and their combinations should be chosen to ensure that the process does not affect the cell cycle progression. Chemical synchronisation methods are suited for obtaining large numbers of synchronised cells.…”

Section: Box 1: Stages Of the Cell Cycle And Mechanism Of Cell Cycle Regulatorsmentioning

confidence: 99%

Advances and enabling technologies for phase-specific cell cycle synchronisation

et al. 2022

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Overview of Cell Synchronization

Bánfalvi

2016

Methods in Molecular Biology

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The widespread interest in cell synchronization is maintained by the studies of control mechanism involved in cell cycle regulation. During the synchronization distinct subpopulations of cells are obtained representing different stages of the cell cycle. These subpopulations are then used to study regulatory mechanisms of the cycle at the level of macromolecular biosynthesis (DNA synthesis, gene expression, protein synthesis), protein phosphorylation, development of new drugs, etc. Although several synchronization methods have been described, it is of general interest that scientists get a compilation and an updated view of these synchronization techniques. This introductory chapter summarizes: (1) the basic concepts and principal criteria of cell cycle synchronizations, (2) the most frequently used synchronization methods, such as physical fractionation (flow cytometry, dielectrophoresis, cytofluorometric purification), chemical blockade, (3) synchronization of embryonic cells, (4) synchronization at low temperature, (5) comparison of cell synchrony techniques, (6) synchronization of unicellular organisms, and (7) the effect of synchronization on transfection.

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Synchronization of cancer cell lines with methotrexate in vitro

Cited by 6 publications

References 27 publications

Anti-leishmanial and Anti-cancer Activities of a Pentacyclic Triterpenoid Isolated from the Leaves of <i>Terminalia arjuna</i> Combretaceae

Anti-leishmanial and Anti-cancer Activities of a Pentacyclic Triterpenoid Isolated from the Leaves of <i>Terminalia arjuna</i> Combretaceae

Advances and enabling technologies for phase-specific cell cycle synchronisation

Overview of Cell Synchronization

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