Synchronization of Budding Yeast Cultures

Neymann, Tobias Claus; Wegerhoff, Sven; Engell, Sebastian

doi:10.3182/20110828-6-it-1002.01712

Cited by 2 publications

(1 citation statement)

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“…protein expression) properties. Synchronised yeast cells can also benefit biotechnological applications to optimise cell-cycle modulated production of metabolites and heterologous proteins 9 – 11 .…”

Section: Introductionmentioning

confidence: 99%

Automatic synchronisation of the cell cycle in budding yeast through closed-loop feedback control

et al. 2021

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The cell cycle is the process by which eukaryotic cells replicate. Yeast cells cycle asynchronously with each cell in the population budding at a different time. Although there are several experimental approaches to synchronise cells, these usually work only in the short-term. Here, we build a cyber-genetic system to achieve long-term synchronisation of the cell population, by interfacing genetically modified yeast cells with a computer by means of microfluidics to dynamically change medium, and a microscope to estimate cell cycle phases of individual cells. The computer implements a controller algorithm to decide when, and for how long, to change the growth medium to synchronise the cell-cycle across the population. Our work builds upon solid theoretical foundations provided by Control Engineering. In addition to providing an avenue for yeast cell cycle synchronisation, our work shows that control engineering can be used to automatically steer complex biological processes towards desired behaviours similarly to what is currently done with robots and autonomous vehicles.

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Section: Introductionmentioning

confidence: 99%