Synchronization in mammalian system: An introduction

Sharma, Arun Kumar

doi:10.1007/bf00122157

Cited by 5 publications

(6 citation statements)

References 32 publications

(22 reference statements)

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“…For applying the microprotoplast fusion method in higher plants, it is essential to establish an efficient system for mass-preparation of microprotoplasts. Microprotoplasts of higher plants have been obtained from 2 types of cell populations partially synchronized in the cell cycle: fast-growing cell suspension cultures 9,23 and microsporocytes 4,6 . For the production of intergeneric asymmetric hybrid plants with one or a few alien chromosomes via microprotoplast fusion for genetic improvement and chromosome studies in Liliaceous ornamental plants, we aimed to develop an efficient and reproducible system for mass-preparation of microprotoplasts.…”

Section: Introductionmentioning

confidence: 99%

Preparation of Microprotoplasts for Partial Genome Transfer via Microprotoplast Fusion in Liliaceous Ornamental Plants

Saito

Nakano

2002

JARQ

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We aimed to produce intergeneric hybrid plants with only one or a few alien chromosomes via microprotoplast fusion for genetic improvement and chromosome studies in Liliaceous ornamental plants. In order to apply this technique, it is essential to establish an efficient system for mass-preparation of microprotoplasts. We have established 2 different systems for isolating microprotoplasts, one from partially synchronized cell suspension cultures of Hemerocallis hybrida and the other from developing microspores of Lilium longiflorum. Here, the induction of micronucleated cells, isolation of microprotoplasts, and enrichment of smaller microprotoplasts containing one or a few chromosomes are described for both systems.

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Section: Introductionmentioning

confidence: 99%

Preparation of Microprotoplasts for Partial Genome Transfer via Microprotoplast Fusion in Liliaceous Ornamental Plants

Saito

Nakano

2002

JARQ

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“…Synchrony was evaluated using incorporation of flowcytometery, analysis of metaphase/anaphase index (M/AI) and expression pattern of cell cycle marker genes which differentiates different phases. 11,12,22 Flow-cytometery analysis was done to monitor cell cycle synchronization over a 19 h period after treatment of MM2d cells with Aphidicolin for 24 h and subsequent removal of the block (totally 10 time points). Obtained data indicated that the majority of the cells proceed synchronous through S phase with an S-phase peak of 74% immediately after release (Fig.…”

Section: Mm2d Cell Suspension Culturementioning

confidence: 99%

“…In physical method, cells sort by flow-cytometry based on their size and DNA content. 11,12 Induction of synchrony can be achieved by starvation or nutritional deficiency of essential compounds for growth such as hormones, phosphate, nitrate or sucrose [13][14][15] or by applying specific inhibitors blocking the progression of cell cycle events beyond a given point in the cycle. 12 Utilizing of DNA synthesis inhibitors have been frequent means to obtain synchronized plant cells.…”

Section: Introductionmentioning

confidence: 99%

Identification of transcription factors linked to cell cycle regulation inArabidopsis

Nayeri

2014

Plant Signaling & Behavior

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Cell cycle is an essential process in growth and development of living organisms consists of the replication and mitotic phases separated by 2 gap phases; G1 and G2. It is tightly controlled at the molecular level and especially at the level of transcription. Precise regulation of the cell cycle is of central significance for plant growth and development and transcription factors are global regulators of gene expression playing essential roles in cell cycle regulation. This study has uncovered TFs that are involved in the control of cell cycle progression. With the aid of multi-parallel quantitative RT-PCR, the expression changes of 1880 TFs represented in the Arabidopsis TF platform was monitored in Arabidopsis synchronous MM2d cells during a 19 h period representing different time points corresponding to the 4 cell cycle phases after treatment of MM2d cells with Aphidicolin. Comparative TF expression analyses performed on synchronous cells resulted in the identification of 239 TFs differentially expressed during the cell cycle, while about one third of TFs were constitutively expressed through all time points. Phase-specific TFs were also identified.

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“…So far different methods have been used for the synchronization of cells in cell suspension cultures and root tip meristems (Doležel et al 1992(Doležel et al , 1999Ghosh et al 1999;Kodama et al 1991;Lucretti et al 1993;Magyar et al 2005;Menges and Murray 2002;Nishida et al 1992;Sharma 1999). Commonly, researches induce plant cells to quiescence by serum starvation e.g., phosphate (Kodama et al 1991), hormone (Nishida et al 1992), and carbohydrate starvation (Menges and Murray 2002;Polit et al 2003).…”

Section: Introductionmentioning

confidence: 98%

An improved method for the cell cycle synchronization of Vicia faba root meristem cells

Polit

2007

Acta Physiol Plant

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Plant root meristem cells divide asynchronously which makes biochemical analysis of cell cycle regulation particularly difficult. In the present article a high level of cell cycle synchronization in Vicia faba root meristems was obtained by using a rich medium (HNS), special culture conditions and a double-block method with replication inhibitor-hydroxyurea (HU). Two HU concentrations were tested and different periods of the first and the second synchronization, and of cycle recommencement between the first and the second blockage. The level of synchronization was estimated on the basis of 3 H-thymidine labeling indices, mitotic, and phase indices and indices determining the percentage of G1 and G2 cells, which were identified by cytophotometric measurements of DNA content in individual nuclei. The highest level of cell cycle synchronization was obtained after double treatment of meristems with 1.25 mM HU (18 and 12 h) separated by 6-h incubation in HNS without HU. During the second postincubation in HNS in subsequent hours: 4, 7, 10, 11, over 90% of cells in the S phase, nearly 70% in G2 phase, 86% in mitosis, and nearly 70% in G1 phase were received, respectively. The use of 2.5 mM HU in a similar experimental procedure caused disturbed divisions.

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Synchronization in mammalian system: An introduction

Cited by 5 publications

References 32 publications

Preparation of Microprotoplasts for Partial Genome Transfer via Microprotoplast Fusion in Liliaceous Ornamental Plants

Preparation of Microprotoplasts for Partial Genome Transfer via Microprotoplast Fusion in Liliaceous Ornamental Plants

Identification of transcription factors linked to cell cycle regulation inArabidopsis

An improved method for the cell cycle synchronization of Vicia faba root meristem cells

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