Synchronization dynamics induced on pairs of neurons under applied weak alternating magnetic fields

Azanza, Marı́a J.; Moral, A. del; Calvo, Ana C.; Pérez-Bruzón, R.N.; Junquera, Concepción

doi:10.1016/j.cbpa.2013.08.012

Cited by 5 publications

(2 citation statements)

References 37 publications

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“…Retrospectively, it could be determined that the coupling between large neurons occurs via gap junctions, since also the experimentally mediated MP changes in one influence that of another cell similar to mammals (Kodirov, unpublished). The gap junctions between cells are universal elements among all living being (Bloemendal & Kück, ) and these structures are formed by two hemichannels involving several connexins and pannexins depending on organ (Azanza, del Moral, Calvo, Perez‐Bruzon, & Junquera, ; Honjo et al, ). However, the role of connexins is not limited to only electrical signaling (Voss, Jacobson, Sleigh, Steyn‐Ross, & Steyn‐Ross, ), but they may modulate the function of other channels, for example, there is an interaction between Cx43 and Nav1.5 (Agullo‐Pascual & Delmar, ; Rhett, Ongstad, Jourdan, & Gourdie, ).…”

Section: Introductionmentioning

confidence: 99%

Limulus and heart rhythm

et al. 2018

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Great interest in the comparative physiology of hearts and their functions in Animalia has emerged with classic papers on Limulus polyphemus and mollusks. The recurrent cardiac activity-heart rate-is the most important physiological parameter and when present the kardia (Greek) is vital to the development of entire organs of the organisms in the animal kingdom. Extensive studies devoted to the regulation of cardiac rhythm in invertebrates have revealed that the basics of heart physiology are comparable to mammals. The hearts of invertebrates also beat spontaneously and are supplied with regulatory nerves: either excitatory or inhibitory or both. The distinct nerves and the source of excitation/inhibition at the level of single neurons are described for many invertebrate genera. The vertebrates and a majority of invertebrates have myogenic hearts, whereas the horseshoe crab L. polyphemus and a few other animals have a neurogenic cardiac rhythm. Nevertheless, the myogenic nature of heartbeat is precursor, because the contraction of native and stem-cellderived cardiomyocytes does occur in the absence of any neural elements. Even in L. polyphemus, the heart rhythm is myogenic at embryonic stages.

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Section: Introductionmentioning

confidence: 99%

Limulus and heart rhythm

et al. 2018

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“…These physiological responses through their influence on brain function are harmful, leading to deterioration of the quality of life; thus, MF exposure is an important biological indicator to be considered from the perspective of human body protection. Regarding the influence of low-intensity power frequency MFs on brain function, great interest has been gained and numerous findings have been obtained from various recent studies with human volunteers (Marino et al, 2004 ; Legros et al, 2012 , 2015 ), experimental animals (Manikonda et al, 2007 ; Szemerszky et al, 2010 ; Balassa et al, 2013 ; Rauš et al, 2013 , 2016 ; Salunke et al, 2014 ; Elmas and Comlekci, 2015 ), and cultured cells and tissues (Azanza et al, 2002 , 2013 ; Manikonda et al, 2007 ; Moghadam et al, 2008 , 2011 ; Varró et al, 2009 ; Moretti et al, 2013 ; Gramowski-Voß et al, 2015 ; Yang et al, 2015 ); however, the thresholds of neuronal modulation and implicated mechanisms by MF exposure are still under investigation. In addition, it has been recommended in the environmental health criteria (EHC) published by the World Health Organization (WHO) that the mechanism and threshold in the nervous system should be explained using cultured cells and theoretical models (World Health Organization, 2007 ).…”

Section: Introductionmentioning

confidence: 99%

Response of Cultured Neuronal Network Activity After High-Intensity Power Frequency Magnetic Field Exposure

et al. 2018

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High-intensity and low frequency (1–100 kHz) time-varying electromagnetic fields stimulate the human body through excitation of the nervous system. In power frequency range (50/60 Hz), a frequency-dependent threshold of the external electric field-induced neuronal modulation in cultured neuronal networks was used as one of the biological indicator in international guidelines; however, the threshold of the magnetic field-induced neuronal modulation has not been elucidated. In this study, we exposed rat brain-derived neuronal networks to a high-intensity power frequency magnetic field (hPF-MF), and evaluated the modulation of synchronized bursting activity using a multi-electrode array (MEA)-based extracellular recording technique. As a result of short-term hPF-MF exposure (50–400 mT root-mean-square (rms), 50 Hz, sinusoidal wave, 6 s), the synchronized bursting activity was increased in the 400 mT-exposed group. On the other hand, no change was observed in the 50–200 mT-exposed groups. In order to clarify the mechanisms of the 400 mT hPF-MF exposure-induced neuronal response, we evaluated it after blocking inhibitory synapses using bicuculline methiodide (BMI); subsequently, increase in bursting activity was observed with BMI application, and the response of 400 mT hPF-MF exposure disappeared. Therefore, it was suggested that the response of hPF-MF exposure was involved in the inhibitory input. Next, we screened the inhibitory pacemaker-like neuronal activity which showed autonomous 4–10 Hz firing with CNQX and D-AP5 application, and it was confirmed that the activity was reduced after 400 mT hPF-MF exposure. Comparison of these experimental results with estimated values of the induced electric field (E-field) in the culture medium revealed that the change in synchronized bursting activity occurred over 0.3 V/m, which was equivalent to the findings of a previous study that used the external electric fields. In addition, the results suggested that the potentiation of neuronal activity after 400 mT hPF-MF exposure was related to the depression of autonomous activity of pacemaker-like neurons. Our results indicated that the synchronized bursting activity was increased by hPF-MF exposure (E-field: >0.3 V/m), and the response was due to reduced inhibitory pacemaker-like neuronal activity.

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Effects of 50 Hz magnetic fields on gap junctional intercellular communication in NIH3T3 cells

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Goudeau

et al. 2015

Bioelectromagnetics

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The present study focused on gap junctional intercellular communication (GJIC) as a target for biological effects of extremely low-frequency (ELF) magnetic field (MF) exposure. Fluorescence recovery after photobleaching microscopy (FRAP) was used to visualize diffusion of a fluorescent dye between NIH3T3 fibroblasts through gap junctions. The direct effect of 24 h exposure to 50 Hz MF at 0.4 or 1 mT on GJIC function was assessed in one series of experiments. The potential synergism of MF with an inhibitor of GJIC, phorbol ester (TPA), was studied in another series by observing FRAP when NIH3T3 cells were incubated with TPA for 1 h following 24 h exposure to MF. In contrast to other reports of ELF-MF effects on GJIC, under our experimental conditions we observed neither direct inhibition of GJIC nor synergism with TPA-induced inhibition from 50 Hz MF exposures.

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Synchronization dynamics induced on pairs of neurons under applied weak alternating magnetic fields

Cited by 5 publications

References 37 publications

Limulus and heart rhythm

Limulus and heart rhythm

Response of Cultured Neuronal Network Activity After High-Intensity Power Frequency Magnetic Field Exposure

Effects of 50 Hz magnetic fields on gap junctional intercellular communication in NIH3T3 cells

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