Synchronisation of glycolytic activity in yeast cells

Hauser, M.

doi:10.1007/s00294-021-01214-y

Cited by 11 publications

(10 citation statements)

References 96 publications

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“…Specifically, recent studies on the synchronization among phase oscillators coupled with a coupling strength γ that can be mapped to ε in the present study, have also shown enlarged Arnold tongues with increasing γ [51], indicating the enhanced stability of oscillatory dynamics or chronotaxicity , in other words the ability of a self-sustained oscillator to resist non-autonomous perturbations. Along with these studies [51,52], our study substantiates that the glycolytic oscillation of a single cell can indeed be influenced by the oscillatory metabolic signals from the surrounding cells, and explains the ease of inter-cellular synchronization and enhanced stability of oscillations in cell populations [50,53,54]. However, an excessive amount of glucose injection can bring the system into disarray, rendering the dynamical state of the system chaotic, in other words, hyper-sensitive to initial conditions and unpredictable.…”

Section: Discussionsupporting

confidence: 79%

Glycolytic oscillations under periodic drivings

Kim,

Hyeon

2024

J. R. Soc. Interface.

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In many living organisms displaying circadian rhythms, the intake of energy often occurs in a periodic manner. Glycolysis is a prototypical biochemical reaction that exhibits a self-sustained oscillation under continuous injection of glucose. Here we study the effect of periodic injection of glucose on the glycolytic oscillation from a dynamical systems perspective. In particular, we employ Goldbeter’s allosteric model of phosphofructokinase as a model system for glycolytic oscillations, and explore the effect of periodic substrate influx of varying frequencies and amplitudes by building the phase diagrams of Lyapunov exponents and oscillatory periods. When the frequency of driving is tuned around the harmonic and sub/super-harmonic conditions of the natural frequency, the system is entrained to a frequency-locked state, forming an entrainment band that broadens with an increasing amplitude of driving. On the other hand, if the amplitude is substantial, the system may transition, albeit infrequent, to a chaotic state which defies prediction of dynamical behaviour. Our study offers in-depth understandings into the controllability of glycolytic oscillation as well as explaining physical underpinnings that enable the synchronous oscillations among a dense population of cells.

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Section: Discussionsupporting

confidence: 79%

Glycolytic oscillations under periodic drivings

Kim,

Hyeon

2024

J. R. Soc. Interface.

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“…Glycolytic oscillations are typically induced in yeast by exposure of starved cells to glucose combined with cyanide, although weaker and less sustained oscillations could also be induced by glucose alone 32 , 71 , and they primarily originate due to the allosteric regulation of PFK 18 , 27 , 28 , 30 . However, whether such glycolytic oscillations are common and physiologically significant remains unclear 26 , 33 .…”

Section: Discussionmentioning

confidence: 99%

“…Glycolytic oscillations were observed in other eukaryotic cells, too [23][24][25] . It was proposed that this oscillatory process is controlled primarily by allosteric regulation of phosphofructokinase, with substrate inhibition by ATP and product activation by AMP and fructose 1,6-bisphosphate 18,20,[26][27][28][29][30] , though additional interactions might be involved in regulation of such oscillations and their synchronization between individual cells 21,[31][32][33][34][35][36] . Although glycolytic oscillations were also predicted to occur in prokaryotes [37][38][39] , the only experimental indication for their existence in bacteria was up to now provided by early measurements of metabolite dynamics in the Escherichia coli bioreactor culture exposed to glucose 40 .…”

mentioning

confidence: 99%

Dynamic fluctuations in a bacterial metabolic network

Kargeti

Colin

et al. 2023

Nat Commun

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The operation of the central metabolism is typically assumed to be deterministic, but dynamics and high connectivity of the metabolic network make it potentially prone to generating fluctuations. However, time-resolved measurements of metabolite levels in individual cells that are required to characterize such fluctuations remained a challenge, particularly in small bacterial cells. Here we use single-cell metabolite measurements based on Förster resonance energy transfer, combined with computer simulations, to explore the real-time dynamics of the metabolic network of Escherichia coli. We observe that steplike exposure of starved E. coli to glycolytic carbon sources elicits large periodic fluctuations in the intracellular concentration of pyruvate in individual cells. These fluctuations are consistent with predicted oscillatory dynamics of E. coli metabolic network, and they are primarily controlled by biochemical reactions around the pyruvate node. Our results further indicate that fluctuations in glycolysis propagate to other cellular processes, possibly leading to temporal heterogeneity of cellular states within a population.

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“…Enhancement of the harmonic and sub/super-harmonic entrainments of glycolytic oscillation under the weak-to-medium periodic drivings is manifested as the broadening of the entrainment bands . This finding sub-stantiates that the glycolytic oscillation of a single cell can indeed be influenced by the oscillatory metabolic signals from the surrounding cells, and explains the ease of inter-cellular synchronization and enhanced oscillations in cell populations [46–48]. However, an excessive amount of glucose injection can bring the system into disarray, rendering the dynamical state of the system chaotic, in other words, hyper-sensitive to initial conditions and inherently uncontrollable.…”

Section: Discussionmentioning

confidence: 92%

Glycolytic oscillations under periodic drivings

Kim,

Hyeon

2023

Preprint

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In many living organisms displaying circadian rhythms, the intake of energy often occurs in a periodic manner. Glycolysis is a prototypical biochemical reaction that exhibits a self-sustained oscillation under continuous injection of glucose. Here we study the effect of periodic injection of glucose on the glycolytic oscillation from a dynamical system perspective. In particular, we employ the Goldbeter’s allosteric model of phosphofructokinase (PFK) as a model system for glycolytic oscillations, and explore the effect of periodic substrate influx of varying frequencies and amplitudes by building the phase diagrams of Lyapunov exponents and oscillatory periods. When the frequency of driving is tuned around the harmonic and sub/super-harmonic conditions of the natural frequency, the system is entrained to a frequency-locked state, forming an entrainment band that broadens with an increasing amplitude of driving. On the other hand, if the amplitude is substantial, the system may transition, albeit infrequent, to a chaotic state which defies both prediction and control of dynamical behavior. Our study offers in-depth understandings into the controllability of glycolytic oscillation as well as explains physical underpinnings that enable the synchronous oscillations among a dense population of cells.

show abstract

Synchronisation of glycolytic activity in yeast cells

Cited by 11 publications

References 96 publications

Glycolytic oscillations under periodic drivings

Glycolytic oscillations under periodic drivings

Dynamic fluctuations in a bacterial metabolic network

Glycolytic oscillations under periodic drivings

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