Synbiotic combination of prebiotic, cacao pod husk pectin and probiotic, Lactobacillus plantarum, improve the immunocompetence and growth of Litopenaeus vannamei

Kuo, Hsin‐Wei; Chang, Chin‐Chyuan; Cheng, Winton

doi:10.1016/j.fsi.2021.09.023

Cited by 24 publications

(22 citation statements)

References 49 publications

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“…In the last twenty years an increasing number of studies show improvements in growth, digestive enzymes and survival after bacterial challenge, changes in the gut microbiome, and enhanced immune capacity of P. vannamei following feeding with lactic acid bacteria alone or in combination with prebiotics, other probiotics, or immune stimulants (Chiu et al, 2007(Chiu et al, , 2021Vieira et al, 2008;Kongnum and Hongpattarakere, 2012;Nguyen et al, 2018;Zheng et al, 2018Zheng et al, , 2020Du et al, 2020;Pooljun et al, 2020;Kuo et al, 2021;Prabawati et al, 2022). Most studies report enhanced growth in the presence of probiotics (e.g., Chiu et al, 2021;Kuo et al, 2021), others (Bernal et al, 2017;Nguyen et al, 2018) including our current study, did not find evidence of such improvements. We observed no changes in the recorded parameters (e.g., growth rate, FCR; Table 2) between the control and L. plantarum diet groups over the 28 day feeding period.…”

Section: Discussionmentioning

confidence: 99%

Antagonistic Activity of Lactic Acid Bacteria Against Pathogenic Vibrios and Their Potential Use as Probiotics in Shrimp (Penaeus vannamei) Culture

et al. 2022

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Probiotic use in aquaculture settings can be an approach for disease control and dietary supplementation. We assessed the antagonistic effect of culture supernatants of lactic acid bacteria on the growth of known shrimp pathogens, Vibrio (Listonella) anguillarum, Vibrio alginolyticus, and V. harveyi, using a quantitative microplate bioassay. Supernatants from Lactobacillus curvatus subsp. curvatus, L. plantarum, and Pediococcus acidolactici significantly inhibited the growth of these vibrios. The active component(s) were heat stable (> 100°C) and resistant to freeze-thawing. Most of this inhibitory activity was brought about by the production of an acid pH; however, there was evidence for other factors playing a role. In the search for novel probiotic bacteria, an organism was isolated from the gastrointestinal tract of healthy whiteleg shrimp (Penaeus vannamei)—identified tentatively as Carnobacterium maltaromaticum. This isolate, however, had less potent vibriocidal activity than the lactic acid bacteria and reduced shrimp survival at a dose of 1 × 107 bacteria/shrimp. During a 28-day feeding trial, juvenile P. vannamei fed with L. plantarum supplemented diets showed no gross changes in growth parameters compared with the control. We suggest that lactic acid bacteria could be incorporated into biofloc formulations to purge the growth of pathogenic vibrios in pond settings, rather than being fed directly to shrimp.

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Section: Discussionmentioning

confidence: 99%

Antagonistic Activity of Lactic Acid Bacteria Against Pathogenic Vibrios and Their Potential Use as Probiotics in Shrimp (Penaeus vannamei) Culture

et al. 2022

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“…The presence of this LAB strain might stimulate the production of endogenous enzymes in the host or contribute to the total enzyme activity of the gut as reported previously in shrimps (Adel et al, 2017;Wanna et al, 2021). Several lines of evidence suggested that the crustacea hemocytes, PO, and lysozyme activity play an important role in generating a protective immune response against bacterial diseases including those caused by vibrios (Oktaviana et al, 2014;Zubaidah et al, 2015;Kuo et al, 2021). Shrimps possess three hemocyte types named hyaline cells, semigranular cells, and granular cells (Johansson et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

“…Shrimps possess three hemocyte types named hyaline cells, semigranular cells, and granular cells (Johansson et al, 2000). Hemocyte count can vary greatly in response to infection, environmental stress, and the life cycle of the animal (Zubaidah et al, 2015;Kuo et al, 2021). An increase in the THC provides enhanced immune capability during a period of stress leading to disease resistance in crustacean (Chiu et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

The Combined Use of Pediococcus pentosaceus and Fructooligosaccharide Improves Growth Performance, Immune Response, and Resistance of Whiteleg Shrimp Litopenaeus vannamei Against Vibrio parahaemolyticus

et al. 2022

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In this study, we evaluated the effect of probiotic bacteria Pediococcus pentosaceus supplemented at different inclusion levels in a control diet [basal diet containing 0.5% fructooligosaccharide (FOS)] on the growth performance, feed conversion ratio, immune response, and the disease resistance of whiteleg shrimp Litopenaeus vannamei juveniles against Vibrio parahaemolyticus. A control diet with 0.5% FOS but without P. pentosaceus supplementation (Control) was prepared. In addition, three other test diets were also formulated: control diet supplemented with P. pentosaceus at (i) 1 × 106 cfu g–1 diet (P1), (ii) 1 × 107 cfu g–1 diet (P2), or (iii) 1 × 108 cfu g–1 diet (P3). After a 60-day feeding trial, the experimental shrimps were challenged with V. parahaemolyticus. The results showed that dietary supplementation of P. pentosaceus significantly improved the growth performance and immune responses of L. vannamei juveniles. The juveniles that were fed with a P2 or P3 diet recorded the maximum increase in the final body weight, final length, weight gain, and survival rate. The total hemocyte counts, phenoloxidase, and lysozyme activity of shrimp fed with either of these two diets were significantly enhanced. The results also showed that juveniles fed with a P2 or P3 diet exhibited significantly lower mortality when challenged with V. parahaemolyticus. Overall results suggested that a combination of P. pentosaceus at the inclusion level of 1 × 107 cfu g–1 diet (P2) and 0.5% FOS could be considered as a potential synbiotic formulation for improving the growth, health, and robustness of L. vannamei.

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“…For instance, in the case of prebiotic cacao pod husk pectin, saccharides such as fucose and rhamnose are postulated to trigger degranulation and activate signalling pathways such as the toll‐like receptors (TRLs) 1 and 3 in haemocytes which, in turn, potentiate the immunocompetence and disease resistance of shrimps treated with prebiotics 315,316 . Increment of proPO and RB activities were also evident along with the significant increase in THC in treated shrimps compared to the untreated controls 121,128,194,285,315,317,318 . Besides, the expression of immune genes, such as Pen‐3a, HSP‐70, STAT, ALF, and proPO, were upregulated as a result of prebiotic inclusion 41,50,193 .…”

Section: Prebioticsmentioning

confidence: 99%

Harnessing the potentialities of probiotics, prebiotics, synbiotics, paraprobiotics, and postbiotics for shrimp farming

Goh

Tan

Law

et al. 2022

Reviews in Aquaculture

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The shrimp farming industry is receiving increasing attention globally. However, extending the frontiers of the industry has been a challenge as high stocking density often increases susceptibility to diseases and compromises shrimp quality. There has been a huge adoption of bacterial‐derived additives in recent decades, particularly in Asia. Accumulating evidence demonstrates the modulatory effect of these functional additives on the animal gut microbiota composition, thus making them potential alternatives to antibiotics in controlling infectious diseases, such as acute hepatopancreatic necrosis disease (AHPND). These functional additives could be introduced in various forms, such as probiotics, synbiotics, paraprobiotics, and postbiotics. This article aims to critically review previous studies that investigated the effects of these bacterial derivatives, particularly on shrimp species. The surge of research in recent years indirectly attested to the immense potentialities of these functional additives. Generally, the introduction of bacterial derivatives not only helped in disease control but also demonstrated encouraging outcomes, including better survival rate, immune enhancement, and growth promotion effects. It is interesting to note that the effectiveness of probiotics extended beyond the viability of the bacteria, thus leading to concepts such as paraprobiotics and postbiotics. Although this is a relatively new research area with limited information, this review aims to consolidate available data and provide a comprehensive overview of the state of the art of this research arena. The challenges in each research domain were also discussed towards the end of this review to provide insights into potential future research niches.

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Synbiotic combination of prebiotic, cacao pod husk pectin and probiotic, Lactobacillus plantarum, improve the immunocompetence and growth of Litopenaeus vannamei

Cited by 24 publications

References 49 publications

Antagonistic Activity of Lactic Acid Bacteria Against Pathogenic Vibrios and Their Potential Use as Probiotics in Shrimp (Penaeus vannamei) Culture

Antagonistic Activity of Lactic Acid Bacteria Against Pathogenic Vibrios and Their Potential Use as Probiotics in Shrimp (Penaeus vannamei) Culture

The Combined Use of Pediococcus pentosaceus and Fructooligosaccharide Improves Growth Performance, Immune Response, and Resistance of Whiteleg Shrimp Litopenaeus vannamei Against Vibrio parahaemolyticus

Harnessing the potentialities of probiotics, prebiotics, synbiotics, paraprobiotics, and postbiotics for shrimp farming

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