Synaptotagmin1 is required for spindle stability and metaphase-to-anaphase transition in mouse oocytes

Zhu, Xiaoyan; Qi, Shu-Tao; Li, Jun; Chen, Lei; Zhang, Chunhui; Yang, Shang-Wu; Ouyang, Ying‐Chun; Hou, Yi; Schatten, Heide; Song, Yali; Xing, Fu-qi; Sun, Qing‐Yuan

doi:10.4161/cc.11.4.19329

Cited by 10 publications

(13 citation statements)

References 32 publications

(34 reference statements)

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“…After GVBD, microtubules assemble to chromosomes during the prometaphase I (pro MI) stage, and then chromosomes migrate to the central plate of the bipolar spindle and remain in a well-aligned state during the MI stage. 12,13 Subsequently, anaphase I (AI) ensues, followed by extrusion of the first polar body and the spindle migrates to the cortex. Then, the oocyte emits the first polar body, followed by the formation of the metaphase II (MII) spindle located beneath the plasma membrane.…”

Section: Introductionmentioning

confidence: 99%

SLX2 interacting with BLOS2 is differentially expressed during mouse oocyte meiotic maturation

Zhuang

Shi

et al. 2014

Cell Cycle

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Gametogenesis is a complex biological process of producing cells for sexual reproduction. Xlr super family members containing a conserved COR1 domain play essential roles in gametogenesis. In the present study, we identified that Slx2, a novel member of Xlr super family, is specifically expressed in the meiotic oocytes, which is demonstrated by western blotting and immunohistochemistry studies. In the first meiotic prophase, SLX2 is unevenly distributed in the nuclei of oocytes, during which phase SLX2 is partly co-localized with SYCP3 in synaptonemal complex and γH2AX in the nucleus of oocytes. Interestingly, the localization of SLX2 was found to be switched into the cytoplasm of oocytes after prometaphase I during oocyte maturation. Furthermore, yeast two-hybrid and coimmunoprecipitation studies demonstrated that SLX2 interacts with BLOS2, which is a novel centrosome-associated protein, and co-localized with γ-Tubulin, which is a protein marker of chromosome segregation in meiosis. These results indicated that SLX2 might get involved in chromosomes segregation during meiosis by interaction with BLOS2. In conclusion, SLX2 might be a novel gametogenesis-related protein that could play multiple roles in regulation of meiotic processes including synaptonemal complex assembly and chromosome segregation.

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Section: Introductionmentioning

confidence: 99%

SLX2 interacting with BLOS2 is differentially expressed during mouse oocyte meiotic maturation

Zhuang

Shi

et al. 2014

Cell Cycle

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“…Sty1 and CGs displayed a complete synchronized change when mouse oocytes were activated. Our previous results have shown that Syt1 knockdown disturbed the metaphase-anaphase transition and PB1 extrusion (P < 0.05) (Zhu et al, 2012). However, some oocytes still extruded the PB1 after Syt1 knockdown; this may be because oocytes showed different degrees of spindle disorganization and chromosome misalignment and those with only a slight damage still extruded the PB1 after prolonged culture.…”

Section: Discussionmentioning

confidence: 89%

“…6) is the cause of the perturbed Ca 2þ signals and CGs exocytosis, therefore inducing the structural changes of Syt1-MO. Our previous studies showed that Syt1 might act as an MTOC-associated protein and play an important role in coordinating MTOC and microtubule functions (Zhu et al, 2012). .…”

Section: Discussionmentioning

confidence: 99%

“…All chemicals and culture medium were purchased from Sigma Chemical Company (St. Louis, MO, USA) except for those specifically mentioned. Oocyte collection and culture were the same as previously described (Zhu et al, 2012).…”

Section: Methodsmentioning

confidence: 99%

“…Microinjection of Syt1 or control morpholinol antisense oligos MO and lifeact-green fluorescent protein (GFP) mRNA MO sequence (Gene Tools) of Syt1-MO (GENE TOOLS, LLC, 5 0 -GACTGGCACTCACCATTTTTGGTTC-3 0 ) and control-MO (GENE TOOLS, LLC, 5 0 -CCTCTTACCTCAgTTACAATTT-ATA-3 0 ) was designed as previously described. Microinjections were performed as previously reported (Zhu et al, 2012). To examine how Syt1 knockdown disrupted the mouse oocyte activation process and injected 1 mM Syt1-MO or control-MO into GV oocytes.…”

Section: Parthenogenetic Activationmentioning

confidence: 99%

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Synaptotagmin 1 regulates cortical granule exocytosis during mouse oocyte activation

Zhu

Zhang

et al. 2019

Zygote

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Synaptotagmin 1 (Syt1) is an abundant and important presynaptic vesicle protein that binds Ca 2þ for the regulation of synaptic vesicle exocytosis. Our previous study reported its localization and function on spindle assembly in mouse oocyte meiotic maturation. The present study was designed to investigate the function of Syt1 during mouse oocyte activation and subsequent cortical granule exocytosis (CGE) using confocal microscopy, morpholinol-based knockdown and time-lapse live cell imaging. By employing live cell imaging, we first studied the dynamic process of CGE and calculated the time interval between [Ca 2þ ]i rise and CGE after oocyte activation. We further showed that Syt1 was co-localized to cortical granules (CGs) at the oocyte cortex. After oocyte activation with SrCl 2 , the Syt1 distribution pattern was altered significantly, similar to the changes seen for the CGs. Knockdown of Syt1 inhibited [Ca 2þ ]i oscillations, disrupted the F-actin distribution pattern and delayed the time of cortical reaction. In summary, as a synaptic vesicle protein and calcium sensor for exocytosis, Syt1 acts as an essential regulator in mouse oocyte activation events including the generation of Ca 2þ signals and CGE.

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Compensatory endocytosis occurs after cortical granule exocytosis in mouse eggs

Gómez-Elías

Fissore

Cuasnicú

et al. 2019

Journal Cellular Physiology

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Compensatory endocytosis (CE) is one of the primary mechanisms through which cells maintain their surface area after exocytosis. Considering that in eggs massive exocytosis of cortical granules (CG) takes place after fertilization, the aim of this study was to evaluate the occurrence of CE following cortical exocytosis in mouse eggs. For this purpose, we developed a pulse-chase assay to detect CG membrane internalization. Results showed internalized labeling in SrCl 2 -activated and fertilized eggs when chasing at 37°C, but not at a nonpermissive temperature (4°C). The use of kinase and calcineurin inhibitors led us to conclude that this internal labeling corresponded to CE. Further experiments showed that CE in mouse eggs is dependent on actin dynamics and dynamin activity, and could be associated with a transient exposure of phosphatidylserine. Finally, CE was impaired in A23187 ionophore-activated eggs, highlighting once again the mechanistic differences between the activation methods. Altogether, these results demonstrate for the first time that egg activation triggers CE in mouse eggs after exocytosis of CG, probably as a plasma membrane homeostasis mechanism.

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Synaptotagmin1 is required for spindle stability and metaphase-to-anaphase transition in mouse oocytes

Cited by 10 publications

References 32 publications

SLX2 interacting with BLOS2 is differentially expressed during mouse oocyte meiotic maturation

SLX2 interacting with BLOS2 is differentially expressed during mouse oocyte meiotic maturation

Synaptotagmin 1 regulates cortical granule exocytosis during mouse oocyte activation

Compensatory endocytosis occurs after cortical granule exocytosis in mouse eggs

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