Synaptophysin is a selective marker for axons in human cutaneous end organ complexes

García‐Mesa, Yolanda; García‐Piqueras, Jorge; Cuendias, Patricia; Cobo, Ramón; Martín‐Cruces, José; Feito, Jorge; García‐Suárez, Olivia; Biedma, Benjamín Martín; Vega, J.A.

doi:10.1016/j.aanat.2022.151955

Cited by 5 publications

(3 citation statements)

References 43 publications

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“…Moreover, in a cellular model of AD, treatment with triptolide increased the expression of synaptophysin in hippocampal neurons ( Nie et al, 2012 ). Synaptophysin is an important protein of synaptic vesicle membrane and is mainly expressed at the presynaptic endings of the nervous system ( García-Mesa et al, 2022 ). Recently, a study reported that intraperitoneally injected triptolide increased hippocampal neuroligin-1 (a cell adhesion protein, located on the excitatory postsynaptic membrane) expression in the APP/PS1 model of AD through epigenetic mechanisms ( Lu et al, 2019 ).…”

Section: Effects Of Triptolide and Celastrol On Neurodegenerative Dis...mentioning

confidence: 99%

The therapeutic potential of triptolide and celastrol in neurological diseases

2022

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Neurological diseases are complex diseases affecting the brain and spinal cord, with numerous etiologies and pathogenesis not yet fully elucidated. Tripterygium wilfordii Hook. F. (TWHF) is a traditional Chinese medicine with a long history of medicinal use in China and is widely used to treat autoimmune and inflammatory diseases such as systemic lupus erythematosus and rheumatoid arthritis. With the rapid development of modern technology, the two main bioactive components of TWHF, triptolide and celastrol, have been found to have anti-inflammatory, immunosuppressive and anti-tumor effects and can be used in the treatment of a variety of diseases, including neurological diseases. In this paper, we summarize the preclinical studies of triptolide and celastrol in neurological diseases such as neurodegenerative diseases, brain and spinal cord injury, and epilepsy. In addition, we review the mechanisms of action of triptolide and celastrol in neurological diseases, their toxicity, related derivatives, and nanotechnology-based carrier system.

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Section: Effects Of Triptolide and Celastrol On Neurodegenerative Dis...mentioning

confidence: 99%

The therapeutic potential of triptolide and celastrol in neurological diseases

2022

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“…The number of synapses can be estimated by measuring the expression of synaptic markers of neuronal connectivity [ 62 ]. SYP constitutes a major protein of synaptic vesicle membrane, present abundantly in presynaptic endings of the CNS, where it is involved in synapse formation and transmission [ 63 ]. PSD95 is a postsynaptic protein, responsible for morphological maturation of adult-born neurons and for the formation of dendritic spines [ 62 ].…”

Section: Discussionmentioning

confidence: 99%

MDPV (3,4-methylenedioxypyrovalerone) administered to mice during development of the central nervous system produces persistent learning and memory impairments

Kuczyńska,

Bartkowska,

Djavadian

et al. 2024

Pharmacol. Rep

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Background Synthetic cathinones (SC) constitute the second most frequently abused class of new psychoactive substances. They serve as an alternative to classic psychostimulatory drugs of abuse, such as methamphetamine, cocaine, or 3,4-methylenedioxymethamphetamine (MDMA). Despite the worldwide prevalence of SC, little is known about their long-term impact on the central nervous system. Here, we examined the effects of repeated exposure of mice during infancy, to 3,4-methylenedioxypyrovalerone (MDPV), a SC potently enhancing dopaminergic neurotransmission, on learning and memory in young adult mice. Methods All experiments were performed on C57BL/6J male and female mice. Animals were injected with MDPV (10 or 20 mg/kg) and BrdU (bromodeoxyuridine, 25 mg/kg) during postnatal days 11–20, which is a crucial period for the development of their hippocampus. At the age of 12 weeks, mice underwent an assessment of various types of memory using a battery of behavioral tests. Afterward, their brains were removed for detection of BrdU-positive cells in the dentate gyrus of the hippocampal formation with immunohistochemistry, and for measurement of the expression of synaptic proteins, such as synaptophysin and PSD95, in the hippocampus using Western blot. Results Exposure to MDPV resulted in impairment of spatial working memory assessed with Y-maze spontaneous alternation test, and of object recognition memory. However, no deficits in hippocampus-dependent spatial learning and memory were found using the Morris water maze paradigm. Consistently, hippocampal neurogenesis and synaptogenesis were not interrupted. All observed MDPV effects were sex-independent. Conclusions MDPV administered repeatedly to mice during infancy causes learning and memory deficits that persist into adulthood but are not related to aberrant hippocampal development.

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“…Gracia-Mesa et al have shown that in the human fetal skin Merkel cells exhibit the expression of Synaptophysin, a typical presynaptic vesicle protein ( García-Mesa et al, 2022b ). In addition, the authors revealed the positivity of fetal Merkel cells for Piezo2, which is an essential component in mechanotransductive mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Human fetal skin derived merkel cells display distinctive characteristics in vitro and in bio-engineered skin substitutes in vivo

Michalak‐Micka

Rütsche

Mazzone

et al. 2022

Front. Bioeng. Biotechnol.

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Human skin contains specialized neuroendocrine Merkel cells responsible for fine touch sensation. In the present study, we performed in-depth analysis of Merkel cells in human fetal back skin. We revealed that these Merkel cells expressed cytokeratin 20 (CK20), were positive for the neuroendocrine markers synaptophysin and chromogranin A, and the mechanosensitive ion channel Piezo2. Further, we demonstrated that Merkel cells were present in freshly isolated human fetal epidermal cells in vitro, and in tissue-engineered human dermo-epidermal skin substitutes 4 weeks after transplantation on immune-compromised rats. Merkel cells retained the expression of CK20, synaptophysin, chromogranin A, and Piezo2 after isolation and in culture, and in the skin substitutes after transplantation. Interestingly, we observed that in fetal skin and in skin substitutes, only Merkel cells were positive for CK8, while in culture, also non-Merkel cells showed positivity for CK8. In summary, human fetal Merkel cells showed phenotypical features confirming their cell identity. This findings are of pivotal importance for the future application of fetal tissue-engineered skin in clinics.

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Synaptophysin is a selective marker for axons in human cutaneous end organ complexes

Cited by 5 publications

References 43 publications

The therapeutic potential of triptolide and celastrol in neurological diseases

The therapeutic potential of triptolide and celastrol in neurological diseases

MDPV (3,4-methylenedioxypyrovalerone) administered to mice during development of the central nervous system produces persistent learning and memory impairments

Human fetal skin derived merkel cells display distinctive characteristics in vitro and in bio-engineered skin substitutes in vivo

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