Synaptonemal complex karyotype of zebrafish

Wallace, B. M. N.; Wallace, H.

doi:10.1038/sj.hdy.6800184

Cited by 57 publications

(42 citation statements)

References 26 publications

(26 reference statements)

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“…6A-7), unlike mouse and guinea pig pachytene spermatocytes in which g-H2AX signals are present but restricted to the XY chromatin. This observation is consistent with previous observations that no heteromorphic sex chromosomes exist in zebrafish (Traut and Winking, 2001;Wallace and Wallace, 2003). Hence, g-H2AX patterns are a powerful tool to characterize the timing and distribution of DSBs in zebrafish spermatogenesis.…”

Section: Expression and Localization Of G-h2ax Is Impaired In Isa Andsupporting

confidence: 92%

Isolation and cytogenetic characterization of zebrafish meiotic prophase I mutants

Saito

Siegfried

Nüsslein‐Volhard

et al. 2011

Developmental Dynamics

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We describe here the isolation and cytogenetic characterization of three meiotic prophase I mutants, denoted ietsugu (its), iesada (isa), and iemochi (imo), isolated by a novel N-ethyl-N-nitrosourea mutagenesis screen for adult zebrafish gonadogenesis. Histological examination and flow cytometry analysis of testes from these mutants showed that each contained neither spermatids nor sperm. Staining for Sycp3 and cleaved Caspase-3 and TUNEL (terminal deoxynucleotidyl transferase-mediated deoxyuridinetriphosphate nick end-labeling) assay further revealed that its had defects at the onset of meiosis, and that isa and imo spermatocytes failed to progress past the zygotene stage with apoptosis occurring in the testicular somatic cells. Staining for phosphorylated histone H2AX showed that foci formation in leptotene spermatocytes was disrupted in isa and imo. Furthermore, in vitro differentiation experiments revealed the possibility that the defects and sterility associated with mutations were germ line autonomous. Our results thus indicate that each responsible gene is necessary for meiotic progression during spermatogenesis and for male fertility.

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Section: Expression and Localization Of G-h2ax Is Impaired In Isa Andsupporting

confidence: 92%

Isolation and cytogenetic characterization of zebrafish meiotic prophase I mutants

Saito

Siegfried

Nüsslein‐Volhard

et al. 2011

Developmental Dynamics

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“…6). By contrast, the gene family in zebrafish (Wallace and Wallace 2003), medaka (Ueda and Naoi 1999), and opossum (Rens et al 2001) is located near the centromere. To examine whether other genes present in subtelomeric regions of different chromosomes are similarly conserved across eutherian species, we carried out synteny analysis between human, mouse, and dog genomes using the Ensembl genome browser.…”

Section: Chromosomal Location Of the Ighv Multigene Familymentioning

confidence: 99%

Evolutionary dynamics of the immunoglobulin heavy chain variable region genes in vertebrates

et al. 2008

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Immunoglobulin heavy chains are polypeptides encoded by four genes: variable (IGHV), joining (IGHJ), diversity (IGHD), and constant (IGHC) region genes. The number of IGHV genes varies from species to species. To understand the evolution of the IGHV multigene family, we identified and analyzed the IGHV sequences from 16 vertebrate species. The results show that the numbers of functional and nonfunctional IGHV genes among different species are positively correlated. The number of IGHV genes is relatively stable in teleosts, but the intragenomic sequence variation is generally higher in teleosts than in tetrapods. The IGHV genes in tetrapods can be classified into three phylogenetic clans (I, II, and III). The clan III and/or II genes are relatively abundant, whereas clan I genes exist in small numbers or are absent in most species. The genomic organization of clan I, II, and III IGHV genes varies considerably among species, but the entire IGHV locus seems to be conserved in the subtelomeric or near-centromeric region of chromosome. The presence or absence of specific IGHV clan members and the lineage-specific expansion and contraction of IGHV genes indicate that the IGHV locus continues to evolve in a species-specific manner. Our results suggest that the evolution of IGHV multigene family is more complex than previously thought and that several factors may act synergistically for the development of antibody repertoire.

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“…Since chromosome spreads with high resolution have already been reported in fish [46,47], we applied these methods to medaka spermatocytes. However, the resulting spreads did not react to the antibodies against MeSYCP1 and MeSYCP3, because of the loss of antigenicity due to strong spreading conditions during the sample preparation.…”

Section: Localization Of Mesycp1 and Mesycp3 On Meiotic Chromosomes Imentioning

confidence: 99%

Structural components of the synaptonemal complex, SYCP1 and SYCP3, in the medaka fish Oryzias latipes

Iwai

Yoshii

Yokota

et al. 2006

Experimental Cell Research

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1 SummaryThe synaptonemal complex (SC) is a meiosis-specific structure essential for synapsis of homologous chromosomes. For the first time in any non-mammalian vertebrates, we have isolated cDNA clones encoding two structural components of the SC, SYCP1 and SYCP3, in the medaka, and investigated their protein expression during gametogenesis.As in the case of mammals, medaka SYCP1 and SYCP3 are expressed solely in meiotically dividing cells. In the diplotene stage, SYCP1 is diminished at desynaptic regions of chromosomes and completely lost on the chromosomes at later stages.SYCP3 is localized along the arm and centromeric regions of chromosomes at metaphase I, and its existence on the whole chromosomes persists up to anaphase I, a situation different from that reported in the mouse, in which SYCP3 is confined to the centromeric regions but lost on the arm regions at metaphase I. Thus, the expression patterns of SC components are different in mammals and fish despite the resemblance in morphological structure of the SC, suggesting divergence in the function of the SC in regulation of meiosis-specific chromosomal behavior. Since the antibody against medaka SYCP3 is cross-reactive to other fishes, it should be generally useful for a meiosis-specific marker in fish germ cells.

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Synaptonemal complex karyotype of zebrafish

Cited by 57 publications

References 26 publications

Isolation and cytogenetic characterization of zebrafish meiotic prophase I mutants

Isolation and cytogenetic characterization of zebrafish meiotic prophase I mutants

Evolutionary dynamics of the immunoglobulin heavy chain variable region genes in vertebrates

Structural components of the synaptonemal complex, SYCP1 and SYCP3, in the medaka fish Oryzias latipes

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