Synaptonemal complex antigen location and conservation.

Møens, Peter B.; Heyting, C.; Dietrich, A.J.J.; Raamsdonk, W. van; Chen, Q

doi:10.1083/jcb.105.1.93

Cited by 116 publications

(66 citation statements)

References 30 publications

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“…In tomato, the MRE11 foci are located primarily but not exclusively in the distal euchromatic segments of the chromosomes, and up to late pachynema, their association with the SC is stable even after DNase treatment ( Fig. 4; Moens et al 1987Moens et al , 2002Anderson et al 1997Anderson et al , 2001). As pachynema proceeds, the proportion of MRE11 foci associated with chromatin increases, again consistent with the abrupt loss of ENs from SCs during early pachynema (Stack and Anderson 1986a;Anderson and Stack 2005).…”

Section: Discussionmentioning

confidence: 99%

Cytological analysis of MRE11 protein during early meiotic prophase I in Arabidopsis and tomato

et al. 2008

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Early recombination nodules (ENs) are multiprotein complexes that are thought to be involved in synapsis and recombination, but little is known about their components or how they may be involved in these events. In this study, we describe the cytological behavior of a possible EN component, MRE11, a protein that is important for the repair of the numerous, programmed deoxyribonucleic acid double-strand breaks (DSBs) that occur early in the meiotic prophase. By immunofluorescence, many MRE11 foci were associated with chromosomal axes during early prophase I in both wild-type Arabidopsis and tomato primary microsporocytes. Similar patterns of MRE11 foci were observed in two Arabidopsis mutants (Atspo11-1 and Atprd1) that are defective in DSB formation and synapsis. In tomato chromosomes, MRE11 foci were more common in distal euchromatin than in proximal heterochromatin, consistent with known EN patterns. However, electron microscopic immunogold localization demonstrated that only about 10% of ENs were labeled, and most MRE11 label was associated with synaptonemal complex components. Thus, in plants, MRE11 foci are not dependent on DSB formation, and most MRE11 foci do not correspond to ENs. More generally, our results show that the simple presence of large numbers of fluorescent foci associated with synapsing chromosomes is insufficient evidence to equate these foci with ENs.

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Section: Discussionmentioning

confidence: 99%

Cytological analysis of MRE11 protein during early meiotic prophase I in Arabidopsis and tomato

et al. 2008

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“…The anti-Rad51 rabbit polyclonal antibody (Ab-1) was purchased from Oncogene Research Products. Antibody incubation and detection procedures were a modification of the protocol of Moens et al (1987) as described by Keegan et al (1996). Combinations of donkey anti-rabbit and donkey antigoat IgG secondary antibodies conjugated to either FITC or TRITC were used for detection (Jackson ImmunoResearch Laboratories).…”

Section: Meiotic Chromosome Stainingmentioning

confidence: 99%

Epithelial cancer in Fanconi anemia complementation group D2 (Fancd2) knockout mice

Houghtaling¹,

Timmers²,

Noll³

et al. 2003

Genes Dev.

269

298

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Fanconi anemia (FA) is a genetic disorder characterized by hypersensitivity to DNA damage, bone marrow failure, congenital defects, and cancer. To further investigate the in vivo function of the FA pathway, mice with a targeted deletion in the distally acting FA gene Fancd2 were created. Similar to human FA patients and other FA mouse models, Fancd2 mutant mice exhibited cellular sensitivity to DNA interstrand cross-links and germ cell loss. In addition, chromosome mispairing was seen in male meiosis. However, Fancd2 mutant mice also displayed phenotypes not observed in other mice with disruptions of proximal FA genes. These include microphthalmia, perinatal lethality, and epithelial cancers, similar to mice with Brca2/Fancd1 hypomorphic mutations. These additional phenotypes were not caused by defects in the ATM-mediated S-phase checkpoint, which was intact in primary Fancd2 mutant fibroblasts. The phenotypic overlap between Fancd2-null and Brca2/Fancd1 hypomorphic mice is consistent with a common function for both proteins in the same pathway, regulating genomic stability.[Keywords: Fanconi anemia; cancer; Fancd2; Brca2; DNA repair; chromosome pairing] Supplemental material is available at http://www.genesdev.org.

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“…The localization of rat/mouse SCP3 (Moens et al, 1987;Lammers et al, 1994), also called COR1 in hamster (Dobson et al, 1994), has been mainly studied in surface-spread rodent spermatocytes. SCP3 first appears at AEs in leptotene spermatocytes, and is found at LEs during pairing and synapsis during zygotene and pachytene, respectively.…”

Section: Introductionmentioning

confidence: 99%

Involvement of the cohesin Rad21 and SCP3 in monopolar attachment of sister kinetochores during mouse meiosis I

Parra

Viera

Gómez

et al. 2004

Journal of Cell Science

152

179

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SCP3 is a meiosis-specific structural protein appearing at axial elements and lateral elements of the synaptonemal complex. We have analysed the behaviour of SCP3 and the cohesin subunit Rad21 in mouse spermatocytes by means of a squashing technique. Our results demonstrate that both proteins colocalize and are partially released from chromosome arms during late prophase I stages, although they persist at the interchromatid domain of metaphase I bivalents. Thus, Rad21 cannot be considered a `mitotic'-specific variant, but coexists with Rec8. During late prophase I SCP3 and Rad21 accumulate at centromeres, and together with the chromosomal passenger proteins INCENP and aurora-B kinase, show a complex `double cornet'-like distribution at the inner domain of metaphase I centromeres beneath the associated sister kinetochores. We have observed that Rad21 and SCP3 are displaced from centromeres during telophase I when sister kinetochores separate, and are not present at metaphase II centromeres. Thus, we hypothesise that Rad21, and the superimposed SCP3 and SCP2, are involved in the monopolar attachment of sister kinetochores during meiosis I, and are not responsible for the maintenance of sister-chromatid centromere cohesion during meiosis II as previously suggested.

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Synaptonemal complex antigen location and conservation.

Cited by 116 publications

References 30 publications

Cytological analysis of MRE11 protein during early meiotic prophase I in Arabidopsis and tomato

Cytological analysis of MRE11 protein during early meiotic prophase I in Arabidopsis and tomato

Epithelial cancer in Fanconi anemia complementation group D2 (Fancd2) knockout mice

Involvement of the cohesin Rad21 and SCP3 in monopolar attachment of sister kinetochores during mouse meiosis I

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