Synaptic inputs from identified bipolar and amacrine cells to a sparsely branched ganglion cell in rabbit retina

Bordt, Andrea S.; Pérez, Diego Moreno; Tseng, Luke; Liu, Weiley Sunny; Neitz, Jay; Patterson, Sara S.; Famiglietti, Edward V.; Marshak, David

doi:10.1017/s0952523819000014

Cited by 14 publications

(13 citation statements)

References 80 publications

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“…29,72 Data Analysis and Visualization Data analysis and 3D rendering were performed using an open-source Matlab program (https:// github.com/neitzlab/sbfsem-tools). 4 The cone pedicle analyses were based on XYZ coordinates of the closed curve control points, connected by Catmull-Rom splines. All other analysis was performed using the X, Y, Z coordinates and radius of the Disc annotations.…”

Section: Annotationmentioning

confidence: 99%

An S-cone circuit for edge detection in the primate retina

Patterson

Kuchenbecker

Anderson

et al. 2019

Preprint

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Midget retinal ganglion cells (RGCs) are the most common RGC type in the primate retina. Their responses mediate both color and spatial vision, yet the specific links between midget RGC responses and visual perception are unclear. Previous research on the dual roles of midget RGCs has focused on those comparing long (L) vs. middle (M) wavelength sensitive cones. However, there is evidence for several other rare midget RGC subtypes receiving S-cone input, but their role in color and spatial vision is uncertain. Here, we confirm the existence of the single S-cone center OFF midget RGC circuit in the central retina of macaque monkey both structurally and functionally, by combining single cell electrophysiology with 3D electron microscopy reconstructions of the upstream circuitry. Like the well-studied L vs. M midget RGCs, the S-OFF midget RGCs have a centersurround receptive field consistent with a role in spatial vision. While spectral opponency in a primate RGC is classically assumed to contribute to hue perception, a role supporting edge detection is more consistent with the S-OFF midget RGC receptive field structure and studies of hue perception.

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Section: Annotationmentioning

confidence: 99%

An S-cone circuit for edge detection in the primate retina

Patterson

Kuchenbecker

Anderson

et al. 2019

Preprint

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“…It also differs from OD1 and OD2 cells, bearing many very small dendritic spines in the perisomatic region (Figure 43). It is most similar to the SB3/OD3 cell (Bordt et al, 2019), but is much larger.…”

Section: Resultsmentioning

confidence: 67%

“…They apparently consist of two broad groups: (a) OD1 and OD2 cells, a number of which are represented in the present sample, and (b) OD3 and OD4 cells, only the latter of which has been found in the present study. An OD3 cell, similar to but smaller than the OD4 cell, was studied by electron microscopy, and was referred to in that study as the SB3 ganglion cell (Bordt et al, 2019).…”

Section: Resultsmentioning

confidence: 99%

Morphological identification and systematic classification of mammalian retinal ganglion cells. I. Rabbit retinal ganglion cells

Famiglietti

2020

J of Comparative Neurology

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Retinal ganglion cells (RGCs) convey visual signals to 50 regions of the brain. For reasons of interest and convenience, they constitute an excellent system for the study of brain structure and function. There is general agreement that, absent a complete “parts list,” understanding how the nervous system processes information will remain an elusive goal. Recent studies indicate that there are 30–50 types of ganglion cell in mouse retina, whereas only a few years ago it was still written that mice and the more visually oriented lagomorphs had less than 20 types of RGC. More than 30 years ago, I estimated that rabbits have about 40 types of RGC. The present study indicates that this number is much too low. I have employed the old but powerful method of Golgi‐impregnation to rabbit retina, studying the range of component neurons in this already well‐studied retinal system. Close quantitative and qualitative analyses of 1,142 RGCs in 26 retinas take into account cell body and dendritic field size, level(s) of dendritic stratification in the retina's inner plexiform layer, and details of dendritic branching. Ninety‐one morphologies are recognized. Of these, at least 32 can be correlated with physiologically studied RGCs, dye‐injected for morphological analysis. It is unlikely that rabbits have 91 types of RGC, but is argued here that this number lies between 60 and 70. The present study provides a “yardstick” for measuring the output of future molecular studies that may be more definitive in fixing the number of RGC types in rabbit retina.

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“…Data analysis and three dimensional rendering were performed using an open-source Matlab (Mathworks, RRID: SCR_001622) program https://github.com/neitzlab/sbfsem-tools RRID: SCR_017350 and Tulip ( https://tulip.labri.fr/site/ ). The image rendering was performed using the RenderApp function 17 . Using the SynapseSphere function, synapses were rendered as unit spheres centered at each synapse annotation’s X, Y and Z coordinates then scaled to optimize visibility.…”

Section: Methodsmentioning

confidence: 99%

Synaptic inputs to displaced intrinsically-photosensitive ganglion cells in macaque retina

Bordt

Patterson

Kuchenbecker

et al. 2022

Sci Rep

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Ganglion cells are the projection neurons of the retina. Intrinsically photosensitive retinal ganglion cells (ipRGCs) express the photopigment melanopsin and also receive input from rods and cones via bipolar cells and amacrine cells. In primates, multiple types of ipRGCs have been identified. The ipRGCs with somas in the ganglion cell layer have been studied extensively, but less is known about those with somas in the inner nuclear layer, the “displaced” cells. To investigate their synaptic inputs, three sets of horizontal, ultrathin sections through central macaque retina were collected using serial block-face scanning electron microscopy. One displaced ipRGC received nearly all of its excitatory inputs from ON bipolar cells and would therefore be expected to have ON responses to light. In each of the three volumes, there was also at least one cell that had a large soma in the inner nuclear layer, varicose axons and dendrites with a large diameter that formed large, extremely sparse arbor in the outermost stratum of the inner plexiform layer. They were identified as the displaced M1 type of ipRGCs based on this morphology and on the high density of granules in their somas. They received extensive input from amacrine cells, including the dopaminergic type. The vast majority of their excitatory inputs were from OFF bipolar cells, including two subtypes with extensive input from the primary rod pathway. They would be expected to have OFF responses to light stimuli below the threshold for melanopsin or soon after the offset of a light stimulus.

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Synaptic inputs from identified bipolar and amacrine cells to a sparsely branched ganglion cell in rabbit retina

Cited by 14 publications

References 80 publications

An S-cone circuit for edge detection in the primate retina

An S-cone circuit for edge detection in the primate retina

Morphological identification and systematic classification of mammalian retinal ganglion cells. I. Rabbit retinal ganglion cells

Synaptic inputs to displaced intrinsically-photosensitive ganglion cells in macaque retina

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