Synaptic dynamics and long-term plasticity at synapses of Purkinje cells onto neighboring Purkinje cells of a mormyrid fish: A dual cell recording study

Zhang, Y.; Magnus, G.; Han, Victor Z.

doi:10.1016/j.neuroscience.2012.08.020

Cited by 4 publications

(10 citation statements)

References 56 publications

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“…Taken together, these results indicate that the electrophysiology of PCs in Gad2 Cre /Ai27 and Gad2 Cre /Ai32 mice is essentially the same as that of control mice (n=3; not shown), and that patch recording per se yields results similar to those observed in other in vitro preparations (Welsh et al, 2011; Zhang et al, 2012). Although the vast majority of our recordings were performed on Gad2 Cre /Ai27 mice, some were done in Gad2 Cre /Ai32 mice for control purposes.…”

Section: Resultssupporting

confidence: 68%

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Bidirectional modulation of deep cerebellar nuclear cells revealed by optogenetic manipulation of inhibitory inputs from Purkinje cells

Han¹,

Magnus²,

Zhang

et al. 2014

Neuroscience

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In the mammalian cerebellum, deep cerebellar nuclear (DCN) cells convey all information from cortical Purkinje cells (PCs) to premotor nuclei and other brain regions. However, how DCN cells integrate inhibitory input from PCs with excitatory inputs from other sources has been difficult to assess, in part due to the large spatial separation between cortical PCs and their target cells in the nuclei. To circumvent this problem we have used a Cre-mediated genetic approach to generate mice in which channelrhodopsin-2 (ChR2), fused with a fluorescent reporter, is selectively expressed by GABAergic neurons, including PCs. In recordings from brain slice preparations from this model, mammalian PCs can be robustly depolarized and discharged by brief photo stimulation. In recordings of postsynaptic DCN cells, photo stimulation of PC axons induces a strong inhibition that resembles these cells' responses to focal electrical stimulation, but without a requirement for the glutamate receptor blockers typically applied in such experiments. In this optogenetic model, laser pulses as brief as 1 ms can reliably induce an inhibition that shuts down the spontaneous spiking of a DCN cell for ∼50 ms. If bursts of such brief light pulses are delivered, a fixed pattern of bistable bursting emerges. If these pulses are delivered continuously to a spontaneously bistable cell, the immediate response to such photostimulation is inhibitory in the cell's depolarized state and excitatory when the membrane has repolarized; a less regular burst pattern then persists after stimulation has been terminated. These results indicate that the spiking activity of DCN cells can be bidirectionally modulated by the optically activated synaptic inhibition of cortical PCs.

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Section: Resultssupporting

confidence: 68%

“…P-Clamp 9 software (Axon) was used for data acquisition and analysis. In some cases, dual cell recordings, in which two cells are simultaneously patched by a pair of recording electrodes, were performed to determine the variability of same cell type to identical manipulations (Zhang et al, 2012). …”

Section: Methodsmentioning

confidence: 99%

Bidirectional modulation of deep cerebellar nuclear cells revealed by optogenetic manipulation of inhibitory inputs from Purkinje cells

Han¹,

Magnus²,

Zhang

et al. 2014

Neuroscience

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“…Intracellular and extracellular recordings from LCp Purkinje cells presented here, together with past in vitro studies of mormyrid Purkinje cells (de Ruiter et al 2006;Han and Bell 2003;Zhang et al 2012;Zhang and Han 2007), suggest that the basic electrophysiological properties of LCp Purkinje cells are quite similar to those described for Purkinje cells in other species. Similar to LCp Purkinje cells, the Purkinje-like MG cells of ELL also exhibit multiple types of all-or-none events.…”

Section: Discussionsupporting

confidence: 66%

“…Other mechanisms, besides plasticity, could also be responsible for shaping EOCD responses in LCp. LCp Purkinje cells receive inhibitory inputs from molecular layer interneurons and also inhibit each other, as shown by in vitro paired recordings (Zhang et al 2012).…”

Section: Discussionmentioning

confidence: 99%

Sensory processing and corollary discharge effects in posterior caudal lobe Purkinje cells in a weakly electric mormyrid fish

Alviña

Sawtell

2014

Journal of Neurophysiology

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Although it has been suggested that the cerebellum functions to predict the sensory consequences of motor commands, how such predictions are implemented in cerebellar circuitry remains largely unknown. A detailed and relatively complete account of predictive mechanisms has emerged from studies of cerebellum-like sensory structures in fish, suggesting that comparisons of the cerebellum and cerebellum-like structures may be useful. Here we characterize electrophysiological response properties of Purkinje cells in a region of the cerebellum proper of weakly electric mormyrid fish, the posterior caudal lobe (LCp), which receives the same mossy fiber inputs and projects to the same target structures as the electrosensory lobe (ELL), a well-studied cerebellum-like structure. We describe patterns of simple spike and climbing fiber activation in LCp Purkinje cells in response to motor corollary discharge, electrosensory, and proprioceptive inputs and provide evidence for two functionally distinct Purkinje cell subtypes within LCp. Protocols that induce rapid associative plasticity in ELL fail to induce plasticity in LCp, suggesting differences in the adaptive functions of the two structures. Similarities and differences between LCp and ELL are discussed in light of these results.

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“…Despite these variances in anatomical structure, histological appearance and cell morphology, the basic functional circuits in the CLp appear to be similar to those throughout the mormyrid cerebellum (Han et al 2006;Meek and Nieuwenhuys 1991;Nicholson 1969a, 1969b;Shi et al 2008): its PCs receive distinctive excitatory inputs from multiple parallel fibers (PFs) and single climbing fibers (CFs) and project locally onto efferent cells (also known as euryden-droid cells) and neighboring PCs via GABAergic inhibition (Meek et al 2008;Zhang et al 2012). Thus, the functional circuitry of the mormyrid cerebellum is similar to that which has been characterized in mammals (Han and Bell 2003;Han et al 2006;Ito 1984).…”

Section: Introductionmentioning

confidence: 99%

Cell type-specific plasticity at parallel fiber synapses onto Purkinje cells in the posterior caudal lobe of the mormyrid fish cerebellum

Zhang

Magnus²,

Han³

2018

Journal of Neurophysiology

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It has been demonstrated that there are two morphological subtypes of Purkinje cells (PCs)-fan-shaped Purkinje cells (fPCs) and multipolar Purkinje cells (mPCs)-in the posterior caudal lobe of the mormyrid fish cerebellum, but whether these cell types are also functionally distinct is unknown. Here, we have used electrophysiological and pharmacological tools in a slice preparation to demonstrate that pairing parallel fiber (PF) and climbing fiber (CF) inputs at a low frequency induces long-term depression (LTD) in fPCs but long-term potentiation (LTP) in mPCs. The induction of plasticity in both cell types required postsynaptic Ca and type 1α metabotropic glutamate receptors. However, the LTD in fPCs was inducted via a calcium/calmodulin-dependent protein kinase II cascade, whereas LTP induction in mPCs required calcineurin. Moreover, the LTD in fPCs and LTP in mPCs were accompanied by changes to the corresponding paired-pulse ratios and their coefficients of variation, suggesting presynaptic modes of expression for the plasticity at PF terminals for both cell types. Hence, the synaptic plasticity at PF synapses onto PCs in the posterior caudal lobe of the mormyrid cerebellum is cell type specific, with both pre- and postsynaptic mechanisms contributing to its induction and expression. NEW & NOTEWORTHY Much has been learnt about the cerebellar long-term depression (LTD) in the cortex. More recent work has shown that long-term potentiation (LTP) is equally important for cerebellar motor learning. Here we report for the first time that plasticity in the mormyrid cerebellum is cell type specific, e.g., following the conventional pairing of parallel and climbing fiber inputs in an in vitro preparation leads to LTD in one Purkinje cell subtype and LTP in another.

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Synaptic dynamics and long-term plasticity at synapses of Purkinje cells onto neighboring Purkinje cells of a mormyrid fish: A dual cell recording study

Cited by 4 publications

References 56 publications

Bidirectional modulation of deep cerebellar nuclear cells revealed by optogenetic manipulation of inhibitory inputs from Purkinje cells

Bidirectional modulation of deep cerebellar nuclear cells revealed by optogenetic manipulation of inhibitory inputs from Purkinje cells

Sensory processing and corollary discharge effects in posterior caudal lobe Purkinje cells in a weakly electric mormyrid fish

Cell type-specific plasticity at parallel fiber synapses onto Purkinje cells in the posterior caudal lobe of the mormyrid fish cerebellum

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