Symptoms of Prunus Necrotic Ringspot Virus on Micrografted Prunus Serrulata Cv. Shirofugen Shoot Cultures

Zilkah, S.; Faingersh, E.; Rotbaum, A.; Spiegel, S.; Stein, A.

doi:10.17660/actahortic.1995.386.23

Cited by 6 publications

(5 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Their objective was to establish an in vitro method for the early screening of virus-resistant peach cultivars obtained through genetic engineering. Zilkah et al (1995) aimed to develop a reliable in vitro indexing system for Prunus necrotic ringspot virus based on in vitro grafting. In both cases healthy shoot tips were grafted on infected material.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, phytoplasma transmission was highly efficient and these transmission rates were higher than inoculation rates in vivo which usually do not exceed 80% (unpublished data). Virus transmission rates after in vitro grafting reported by Zilkah et al (1995) ranged from 74 to 96%. For the in vitro grafting conditions no apparent differences were observed between healthy and phytoplasma-infected material or between Malus and Prunus tissue.…”

Section: Discussionmentioning

confidence: 99%

“…Since then it has also been employed for apple (Alskieff & Villermur, 1978) and for different Prunus species (Martinez et al, 1979;Mosella et al, 1979;Déogratias et al, 1986;Barba et al, 1992). Recently, in vitro grafting has been successfully used for the inoculation of Prunus shoot cultures with Prunus necrotic ring spot virus (Heuss-LaRosa et al, 1995;Zilkah et al, 1995) or Plum pox virus (Lansac et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Phytoplasma transmission by in vitro graft inoculation as a basis for a preliminary screening method for resistance in fruit trees

Jarausch¹,

Lansac²,

Bliot³

et al. 1999

Plant Pathology

View full text Add to dashboard Cite

In vitro grafting was tested as a technique for inoculating Prunus rootstock Prunus marianna GF 8-1 with European stone fruit yellows (ESFY) phytoplasmas and apple rootstock Malus pumila MM106 with apple proliferation (AP) phytoplasmas. In vitro shoot cultures of ESFY-infected Prunus marianna GF 8-1 and AP-infected Malus pumila MM106 were used as graft inoculum to transmit the phytoplasmas to the respective healthy rootstock. Phytoplasma transmission was assessed after a graft contact of 1, 2 or 3 months. Healthy autografts were used as controls to monitor parameters of in vitro grafting. Successful graft union formation ranged from 58 to 79% irrespective of the plant species and the sanitary state of the graft. Pathogen-specific polymerase chain reaction (PCR) was used to test the inoculated rootstocks for the presence of ESFY and AP phytoplasmas, respectively. The rate of ESFY phytoplasma transmission in successful Prunus-grafts increased from 69 to 94% with the time of contact. AP phytoplasma transmission to Malus occurred in 80 to 97% of successful grafts. To our knowledge this is the first report of phytoplasma transmission by grafting in vitro. The results provide a good basis for the establishment of a preliminary in vitro screening method for phytoplasma resistance in Prunus and Malus.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Phytoplasma transmission by in vitro graft inoculation as a basis for a preliminary screening method for resistance in fruit trees

Jarausch¹,

Lansac²,

Bliot³

et al. 1999

Plant Pathology

View full text Add to dashboard Cite

show abstract

“…Until now, micrografting has been widely used for elimination of various plant pathogens such as viruses from citrus (Murashige et al ., ; Roistacher et al ., ; Juárez et al ., ; Sanabam et al ., ), apple (Huang & Millikan, ), peach (Jonard et al ., ; Conejero et al ., ), plum (Conejero et al ., ) and sweet pepper (Katoh et al ., ), viroids from citrus (Roistacher et al ., ), peach (Barba et al ., ) and avocado (Suarez et al ., ), and phytoplasma from jujuba (Wang et al ., ). This technique has also been applied to the studies of biology of grafting (Pina & Errea, ; Gökbayrak et al ., ; Melnyk et al ., ), cell signalling (Turnbull et al ., ; Yin et al ., ), rejuvenation (Revilla et al ., ), plant regeneration following micropropagation (Onay et al ., ; Badalamenti et al ., ), genetic transformation (Almeida et al ., ; Juárez et al ., ) and cryopreservation (Volk et al ., ; Wang et al ., ), in vitro breeding (Bisognin et al ., ), and in vitro biological indexing of viruses and viroids (Tanne et al ., ; Zilkah et al ., ; Pathirana & Mckenzie, ; Kapari‐Isaia et al ., ; Wang & Valkonen, ). Applications of micrografting to crop improvement have been extensively reviewed by Jonard et al .…”

Section: Introductionmentioning

confidence: 99%

Development, histological observations and Grapevine leafroll‐associated virus‐3 localisation in in vitro grapevine micrografts

Hao

Cui

et al. 2017

Annals of Applied Biology

View full text Add to dashboard Cite

Development, histological process and Grapevine leafroll-associated virus-3 localisation were studied in micrografts of three scion/rootstock combinations: healthy/healthy, healthy/infected and infected/healthy. Earlier bud break and faster growth in scions of micrografts were obtained when the healthy shoot segments were used as scions, while earlier bud break in rootstocks and greater fresh weight of roots in micrografts were produced when the healthy shoot segments were used as rootstocks. All histological processes including callus initiation and formation in micrografting conjunctions, and initiation of new cambial cells followed by vascular bundle development connecting scions and rootstocks were similar in micrografts, regardless of the sanitary status of the scions and rootstocks used for micrografting. Virus infection in micrografting conjunctions and systematic infection in micrografts were much more efficient and faster in micrografting combination of the infected scions/healthy rootstocks than in the healthy scions/infected rootstocks. To the best of our knowledge, this is the first report addressing histological process of micrograft development and virus localisation in micrografts. In vitro culture system established in this study facilitates studies on the 'pure' impact of the viral infection on micrografting.

show abstract

“…Micrografting techniques have been applied to detect other viruses, such as Grapevine leafroll‐associated virus‐3 (GLRaV‐3) (Pathirana and McKenzie ) and Prunus necrotic ringspot virus (PNRSV) (Zilkah et al. ), and viroids such as citrus exocortis viroids (CEVd) (Kapari‐Isaia et al. ).…”

Section: Introductionmentioning

confidence: 99%

Abiotic stress improves in vitro biological indexing ofGrapevine leafroll-associated virus-3in red grapevine cultivars

Cui

et al. 2015

Australian Journal of Grape and Wine Research

View full text Add to dashboard Cite

Background and Aims:Virus detection is necessary for in vitro production and propagation of virus-free plants, for in vitro conservation and when material is received from overseas in tissue culture. In vitro biological virus indexing is among the reliable techniques. The aim of the present study was to develop an in vitro graft-free protocol for indexing of Grapevine leafroll-associated virus-3 (GLRaV-3). Methods and Results: In vitro GLRaV-3-infected shoots were cultured on half-strength Murashige and Skoog medium supplemented with either 150 mmol NaCl or 4% polyethylene glycol (PEG) 8000 to induce development of the virus symptoms. After 4 weeks of culture, the proportion of the symptom expression was 86 and 81% for Cabernet Sauvignon, 100 for '6-12-2', 78 and 72% for Red Globe and 70 and 60% for Kyoho in the diseased shoots stressed by 150 mmol NaCl and 4% PEG, respectively. Expression of virus symptoms was more obvious and reliable when in vitro plantlets were stressed by NaCl than by PEG. Conclusions: Salt and drought stress induced by NaCl and PEG 8000 can improve in vitro biological indexing of GLRaV-3 in red grapevine cultivars. Significance of the Study: The techniques developed in the present study would have potential application to GLRaV-3 indexing of red grapevine cultivars.

show abstract

Symptoms of Prunus Necrotic Ringspot Virus on Micrografted Prunus Serrulata Cv. Shirofugen Shoot Cultures

Cited by 6 publications

References 0 publications

Phytoplasma transmission by in vitro graft inoculation as a basis for a preliminary screening method for resistance in fruit trees

Phytoplasma transmission by in vitro graft inoculation as a basis for a preliminary screening method for resistance in fruit trees

Development, histological observations and Grapevine leafroll‐associated virus‐3 localisation in in vitro grapevine micrografts

Abiotic stress improves in vitro biological indexing ofGrapevine leafroll-associated virus-3in red grapevine cultivars

Contact Info

Product

Resources

About