Symptomatology of fungal competitors on oyster mushroom’s spawn packets and in vitro evaluation using phytoextracts and a fungicide

Abstract

An experiment was conducted to find out the fungal competitors and symptom studies in damaged Oyster Mushroom spawn packets at National Mushroom Development and Extension Center, Savar, Dhaka, Bangladesh. A total of nine fungal competitors of oyster mushroom were isolated and identified namely-Trichoderma harzianum Rifai, T. viride Pers. (Green strain), T. viride Pers. (Yellow strain), T. koningii Oudem, Mucor hiemalis Wehmer, Papulaspora byssina Hotson, Neurospora sp. Shear and B.O. Dodge., Aspergillus flavus Link., and Botryodiplodia theobromae Pat. on the basis of microscopic, morphological and cultural characteristics. To produce oyster mushroom in an eco-friendly manner and to find out their antifungal potency, 23 plant species belonging to 19 families were screened out against isolated nine fungal competitors of oyster mushroom. Among 23 extracts, the maximum (44%) mycelial inhibition of T. harzianum was found due to Aegle marmelos whereas Eclipta alba showed the highest mycelial inhibition (62%) of T. viride (Green strain); in case of T. viride (Yellow strain), Cassia tora exhibited the highest mycelial inhibition (39%); Diospyros cordifolia showed the maximum mycelial inhibition (48%) of T. koningii; Curcuma longa (rhizome) gave the maximum mycelial inhibition (90%) of Neurospora sp. There were no significant effects found to control of P.

Molecular characterization and in vitro control measures of fruit rot disease of Sweet pepper

“…A requisite quantity of fungicides was added to the medium with a concentration of 250 ppm, 500 ppm, and 750 ppm. Percentage inhibition of the pathogen was calculated (Shamoli et al, 2016) using the formula: I = (C-T/C)×100 Here, I = percentage of mycelium growth inhibition, C= growth of mycelium in control, T = growth of mycelium in treatment.…”

Molecular characterization and vegetative growth of pathogenic seed-borne fungus, Curvularia lunata of tomato and its in vitro control measures

“…The effect of fungicides, namely Jazz 80 WP (Active ingredient: Ethylene bisdithiocarbamate), Amister Top 325 SC (active ingredients: Azoxystrobin + Difenoconazole), and Tilt 250 EC (active ingredients: Propiconazole) were assessed against C. lunata. A requisite quantity of fungicides was added to the medium to get concentration of 250 ppm, 500 ppm, and 750 ppm; inoculated plates were incubated at 25±2°C (Shamoli et al, 2016). The fungal colony diameter was recorded at 7 dpi.…”

Identification and Fungal Biology of Post-harvest Fungi Associated with Amla Fruits and It's In Vitro Control Measures

“…Data were recorded at 7 dpi. Percent mycelial growth inhibition was calculated using the standard formula, I = {(C-T)/C} ×100; in which I = percentage of vegetative growth inhibition; C = growth of mycelium in control condition; and T = mycelial growth in treatment [17]. Data were checked for normality and homogeneity of variance; an ANOVA test was performed using the statistical package SPSS 16.0.…”