, Wash.), AND MELVIN W. EKLUND. Changes in the microflora of vacuum-packaged, irradiated petrale sole (Eopsetta jordani) fillets stored at 0.5 C. Appl. Microbiol. 14:921-927. 1966.-The microfloral changes of irradiated petrale sole fillets during anaerobic (vacuum-packed in cans) refrigerated storage was determined by the identification of 1,260 microbial isolates to the generic level. The samples were irradiated at 0.0, 0.1, 0.2, 0.3, and 0.4 Mrad from a cobalt-60 gamma source, stored at 0.5 C, and examined periodically for spoilage and total microbial population and composition. The preirradiation flora consisted of Achromobacter, Micrococcus, Pseudomonas, coryneforms, Laciobacillus, and Flavobacterium. Immediately after irradiation, Micrococcus, Achromobacter, coryneforms, and Bacillus were predominant. After storage under vacuum, the spoilage flora of the nonirradiated petrale sole was predominantly Pseudomonas; the spoilage flora of the 0.1-Mrad irradiated samples consisted of Pseudomonas and Lactobacillus; and that of the 0.2-, 0.3-, and 0.4-Mrad samples was almost entirely Lactobacillus. Radiation pasteurization, more recently termed radurization, can eliminate as much as 99.9% of the original bacterial flora present on a food product. Some genera of bacteria are more resistant to radiation than others (24) and, therefore, the initial radiation treatment selects for them. Of the organisms that survive irradiation, only those that are able to multiply in the environment under which the product is stored will be able to cause spoilage. Differences in the storage environment, therefore, may cause changes in the composition of the spoilage flora equal in importance, although not necessarily the same, as those caused by the radiation treatment itself. Vacuum packaging has been shown to cause changes in microflora of some sliced meat products (1, 2). Christiansen and Foster (4) showed that vacuum packaging inhibits the growth of Staphylococcus aureus in cured meats and has little, if any, effect on the growth of Clostridium botulinum. Ingram and Thornley (10) reported that the microflora of irradiated chicken meat