Symphonia globulifera, a Widespread Source of Complex Metabolites with Potent Biological Activities

Fromentin, Yann; Cottet, Kevin; Kritsanida, Marina; Michel, Sylvie; Gaboriaud‐Kolar, Nicolas; Lallemand, Marie‐Christine

doi:10.1055/s-0034-1396148

Cited by 18 publications

(17 citation statements)

References 72 publications

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“…Several antimicrobial compounds have been identified from S. globulifera , most of them unique to this species (Fromentin et al . ). The potential use of these chemical compounds range from HIV‐inhibitory (Gustafson et al .…”

Section: Discussionmentioning

confidence: 97%

“…Symphonia globulifera and related taxa have been widely used in traditional medicine (see Introduction and Fromentin et al . for a review). Several antimicrobial compounds have been identified from S. globulifera , most of them unique to this species (Fromentin et al .…”

Section: Discussionmentioning

confidence: 99%

“…; Fromentin et al . ) the investigation of which can greatly benefit from the availability of a draft genome.…”

Section: Introductionmentioning

confidence: 99%

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Development of genomic tools in a widespread tropical tree, Symphonia globulifera L.f.: a new low‐coverage draft genome, SNP and SSR markers

Olsson

Zonjic

Bautista

et al. 2016

Molecular Ecology Resources

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Population genetic studies in tropical plants are often challenging because of limited information on taxonomy, phylogenetic relationships and distribution ranges, scarce genomic information and logistic challenges in sampling. We describe a strategy to develop robust and widely applicable genetic markers based on a modest development of genomic resources in the ancient tropical tree species Symphonia globulifera L.f. (Clusiaceae), a keystone species in African and Neotropical rainforests. We provide the first low-coverage (11X) fragmented draft genome sequenced on an individual from Cameroon, covering 1.027 Gbp or 67.5% of the estimated genome size. Annotation of 565 scaffolds (7.57 Mbp) resulted in the prediction of 1046 putative genes (231 of them containing a complete open reading frame) and 1523 exact simple sequence repeats (SSRs, microsatellites). Aligning a published transcriptome of a French Guiana population against this draft genome produced 923 high-quality single nucleotide polymorphisms. We also preselected genic SSRs in silico that were conserved and polymorphic across a wide geographical range, thus reducing marker development tests on rare DNA samples. Of 23 SSRs tested, 19 amplified and 18 were successfully genotyped in four S. globulifera populations from South America (Brazil and French Guiana) and Africa (Cameroon and São Tomé island, F = 0.34). Most loci showed only population-specific deviations from Hardy-Weinberg proportions, pointing to local population effects (e.g. null alleles). The described genomic resources are valuable for evolutionary studies in Symphonia and for comparative studies in plants. The methods are especially interesting for widespread tropical or endangered taxa with limited DNA availability.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

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Development of genomic tools in a widespread tropical tree, Symphonia globulifera L.f.: a new low‐coverage draft genome, SNP and SSR markers

Olsson

Zonjic

Bautista

et al. 2016

Molecular Ecology Resources

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“…[ 36 ] Antimicrobial activity of Anani extracts was reported against Staphylococcus aureus [ 37 38 ] and against Enterococcus faecalis ,[ 27 39 ] but not against Pseudomonas aeruginosa . Finally, reports on the ethnopharmacology of the plant both in Africa and in Central and South Americas introduced a list with >40 compounds that were already isolated from different organs of the plant and indicated those that were tested against parasites and microorganisms,[ 40 ] but no reports on the influence of S. globulifera on mice behavior phenotype have been reported so far.…”

Section: Discussionmentioning

confidence: 99%

Mice behavioral phenotype changes after administration of Anani (Symphonia globulifera, clusiaceae), an alternative Latin American and African medicine

et al. 2017

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Background:Anani, (Symphonia globulifera, Clusiaceae), known as chewstick, is a traditional plant occurring in Africa and in Central and South Americas that is used against parasites and microorganisms. Although its use is popular in some of these countries, there is a lack of information related to its influence over behavioral phenotype (BP).Objective:The objective of this study is to evaluate the influence of the administration of the extract obtained from the aerial organs of Anani (EB1257) to male Balb-c mice over BP.Materials and Methods:Open cage observation, open field, and elevated-plus maze apparatuses were used. Evaluations were done 15, 30, 60, 120, and 180 min after intraperitoneal administration of Anani extract.Results:Impairment of general behavior activity, response to touch, tail squeeze, defecation, locomotion and rearing frequency were observed although no signs of hemorrhage or macroscopical alterations of internal organs. Anani is harmful, but not toxic if used in the appropriate doses, yet to be determined to male mice. Impairment of locomotion and defecation was observed, indicating some degree of influence over locomotion, but no alterations in anxiety levels were assessed. Three compounds were previously found in the plant-lupeol (1), β-amyrin (2) and 3-β-hydroxyglutin-5-ene (3), and one is being described for the first time to occur in the species: oleanolic acid (4).Conclusions:The present work contributes in the support of the rational use of Anani, an important Latin American and African alternative medicine, presenting findings that are being reported for the first time.SUMMARY Symphonia globulifera impairs locomotion and defecatin in behavior analysesNo alterations in anxiety was observedOleanolic acid occurs in the species. Abbreviations used: BP: Behavioral phenotype; OF: Open field, EPM: Elevated-plus maze, MMA/ICMBio/SISBIO: Ministério do Meio Ambiente/Instituto Chico Mendes de Conservação da Biodiversidade/Sistema de Autorização e Informação em Biodiversidade, IBAMA/MMA/CGen: Instituto Brasileiro do Meio Ambiente e dos Recursos Naturais Renováveis/Ministério do Meio Ambiente/Conselho de Gestão do Patrimônio Genético, AM: Amazonas State, UNIP: Universidade Paulista, mg: milligram, kg: kilogram, I.P: Intraperitoneal, CEUA/ICS/UNIP: Comissão de Ética no Uso de Animais/Instituto de Ciências da Saúde/Universidade Paulista, LD: Lethal dose, NLD: Nonlethal dose, GBA: General behavior activity, FCHCL3: Fraction chloroform, FBuOH: Fraction buthanol, FH2O: Fraction water, FrHEX: Fraction hexane, FrDCM: Fraction dichloromethane, FrMeOH: Fraction methanol, 13C NMR: Carbon nuclear magnetic resonance, EPA: United States Environmental Protection Agency.

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“…In traditional medicine, a decoction of leaves from this plant is used to manage malaria and several other diseases, including diabetes and skin diseases [ 11 ]. In addition, decoctions and extracts of bark are used as diuretics and antiparasitics, and to treat river blindness, chest complaints, cough in children, gonorrhea, scabies, intestinal worms, and prehepatic jaundice [ 12 , 13 ]. Therefore, the present study was designed to investigate for the first time antiplasmodial activity of endophytic fungi isolated from the bark of S. globulifera.…”

Section: Introductionmentioning

confidence: 99%

Antiplasmodial Properties and Cytotoxicity of Endophytic Fungi from Symphonia globulifera (Clusiaceae)

Ateba

Toghueo

Awantu

et al. 2018

JoF

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There is continuing need for new and improved drugs to tackle malaria, which remains a major public health problem, especially in tropical and subtropical regions of the world. Natural products represent credible sources of new antiplasmodial agents for antimalarial drug development. Endophytes that widely colonize healthy tissues of plants have been shown to synthesize a great variety of secondary metabolites that might possess antiplasmodial benefits. The present study was carried out to evaluate the antiplasmodial potential of extracts from endophytic fungi isolated from Symphonia globulifera against a chloroquine-resistant strain of Plasmodium falciparum (PfINDO). Sixty-one fungal isolates with infection frequency of 67.77% were obtained from the bark of S. globulifera. Twelve selected isolates were classified into six different genera including Fusarium, Paecilomyces, Penicillium, Aspergillus, Mucor, and Bipolaris. Extracts from the 12 isolates were tested against PfINDO, and nine showed good activity (IC50 < 10 μg·mL−1) with three fungi including Paecilomyces lilacinus (IC50 = 0.44 μg·mL−1), Penicillium janthinellum (IC50 = 0.2 μg·mL−1), and Paecilomyces sp. (IC50 = 0.55 μg·mL−1) showing the highest promise. These three isolates were found to be less cytotoxic against the HEK293T cell line with selectivity indices ranging from 24.52 to 70.56. Results from this study indicate that endophytic fungi from Symphonia globulifera are promising sources of hit compounds that might be further investigated as novel drugs against malaria. The chemical investigation of active extracts is ongoing.

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Symphonia globulifera, a Widespread Source of Complex Metabolites with Potent Biological Activities

Cited by 18 publications

References 72 publications

Development of genomic tools in a widespread tropical tree, Symphonia globulifera L.f.: a new low‐coverage draft genome, SNP and SSR markers

Development of genomic tools in a widespread tropical tree, Symphonia globulifera L.f.: a new low‐coverage draft genome, SNP and SSR markers

Mice behavioral phenotype changes after administration of Anani (Symphonia globulifera, clusiaceae), an alternative Latin American and African medicine

Antiplasmodial Properties and Cytotoxicity of Endophytic Fungi from Symphonia globulifera (Clusiaceae)

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