Sympathetically evoked secretory potentials in the parotid gland of the cat.

Emmelin, N.; Grampp, W.; Thesleff, Peter

doi:10.1113/jphysiol.1980.sp013237

Cited by 9 publications

(2 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…(n = 30) with a range of only 180-400 msec. This latency corresponds to the shortest latencies recorded in the in vivo preparation in response to electrical stimulation of nerve trunks (Lundberg, 1955(Lundberg, , 1958Emmelin et al 1980). In contrast, the latencies of the responses to ionophoresis, in this study and as previously reported, were highly variable, depending on both the proximity of the micropipette to the recording electrode and on the depth of the impalement.…”

Section: Latencies Of the Responsescontrasting

confidence: 52%

“…Formerly, any investigation of the functional innervation of the salivary glands has necessitated the use of an in vivo preparation, release of endogenous neurotransmitter being achieved by electrical stimulation of dissected nerve trunks (Lundberg, 1955(Lundberg, , 1958Kagayama & Nishiyama, 1974;Emmelin, Grampp & Thesleff, 1980). Such an in vivo preparation is however difficult and has inherent limitations.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Electrophysiology of mouse parotid acini: effects of electrical field stimulation and ionophoresis of neurotransmitters.

Gallacher¹,

Petersen²

1980

The Journal of Physiology

View full text Add to dashboard Cite

SUMMARY1. Intracellular micro-electrode recordings of membrane potential and input resistance were made from surface acini of mouse parotid glands placed in a Perspex tissue bath through which oxygenated physiological saline solutions were circulated. The acinar cells were stimulated by microionophoresis of both acetylcholine (ACh) and adrenaline (Ad) from extracellular micropipettes, and by electrical field stimulation via a pair of platinum electrodes.2. The acinar cells had a mean resting membrane potential of -64x9 mV + 0x6 s.E. The input resistance of the unstimulated cell was 4-63 MQ + 0419 S.E. In a number of cells spontaneous miniature depolarizations were observed, associated with synchronous reductions in input resistance.3. The responses to ionophoresis of both ACh and Ad and the response to supramaximal field stimulation were identical. Stimulation always evoked a marked decrease in input resistance associated with an initial potential change, generally followed by a delayed hyperpolarization during which the input resistance returned to normal.4. Field-stimulation responses could be evoked to single shock (1-2 msec) and to low frequency (1-4 Hz) stimulation. The latency for this response was 245 (10-5 M) abolished the response to Ad but left the field-stimulation response unaffected.8. Electrical field stimulation of isolated segments of salivary gland evoked release of endogenous neurotransmitter as a consequence of neural excitation. The technique of field stimulation thus makes it possible to investigate the functional innervation of a gland using the in vitro preparation. In the mouse parotid gland the field stimulus response was mediated by ACh released from parasympathetic nerve endings.

show abstract

Section: Latencies Of the Responsescontrasting

confidence: 52%

Section: Introductionmentioning

confidence: 99%

Electrophysiology of mouse parotid acini: effects of electrical field stimulation and ionophoresis of neurotransmitters.

Gallacher¹,

Petersen²

1980

The Journal of Physiology

View full text Add to dashboard Cite

show abstract

Electrophysiology of Salivary Acinar Cells: Microelectrode Studies

Nishiyama¹,

Hayashi²,

Takahashi³

et al. 1990

Epithelial Secretion of Water and Electrolytes

View full text Add to dashboard Cite

Nerve-induced secretion of parotid acinar granules in cats

Emmelin

Garrett

1989

Cell Tissue Res.

View full text Add to dashboard Cite

Electron microscopy of cat parotid glands revealed great heterogeneity in the secretory granules of normal unstimulated acinar cells. Electrical stimulation of the parasympathetic nerve to the gland evoked a copious flow of parotid saliva which was accompanied by an extensive depletion of the secretory granules from the acinar cells. Exocytosis was captured as it was occurring by means of perfusion-fixation, and showed that the events occur in a conventional manner. Stimulation of the sympathetic nerve caused only a very small flow of saliva, and no acinar degranulation was detected. It can be concluded that the parasympathetic secretomotor axons provide the main drive for parotid acinar degranulation in the cat. This contrasts with the rat in which sympathetic impulses provide the main stimulus for parotid acinar degranulation. These dissimilarities serve to emphasise how extensively species differences may influence autonomic responses in salivary glands.

show abstract

Sympathetically evoked secretory potentials in the parotid gland of the cat.

Cited by 9 publications

References 26 publications

Electrophysiology of mouse parotid acini: effects of electrical field stimulation and ionophoresis of neurotransmitters.

Electrophysiology of mouse parotid acini: effects of electrical field stimulation and ionophoresis of neurotransmitters.

Electrophysiology of Salivary Acinar Cells: Microelectrode Studies

Nerve-induced secretion of parotid acinar granules in cats

Contact Info

Product

Resources

About