Sympathetic nerve contact causes maturation of mast cellsin vitro

Blennerhassett, Michael G.; Bienenstock, John

doi:10.1002/(sici)1097-4695(199805)35:2<173::aid-neu4>3.0.co;2-1

Cited by 37 publications

(22 citation statements)

References 26 publications

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“…Our findings are in agreement with the perivascular distribution pattern and the early presence of adrenergic innervation in specific compartments of the thymus and spleen during critical periods of development, reported in other species Blennerhassett and Bienenstock 1998). The latter may suggest a possible role of the sympathetic nervous system in blood flow control and lymphocyte traffic as well as in maturation of the immune system.…”

Section: Discussionsupporting

confidence: 92%

Ontogeny of Intrinsic Innervation in the Human Thymus and Spleen

Anagnostou

Doussis-Anagnostopoulou

Tiniakos

et al. 2007

J Histochem Cytochem.

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S U M M A R YThe ontogeny of the innervation of human lymphoid organs has not been studied in detail. Our aim was to assess the nature and distribution of parenchymal nerves in human fetal thymus and spleen. We used the peroxidase immunohistochemical technique with antibodies specific to neuron-specific enolase (NSE), neurofilaments (NF), PGP9.5, S100 protein, and tyrosine hydroxylase (TH) and evaluated our results with image analysis. In human fetal thymus, NSE-, NF-, S100-, PGP9.5-, and TH-positive nerves were identified associated with large blood vessels from 18 gestational weeks (gw) onwards, increasing in density during development. Their branches penetrated the septal areas at 20 gw, reaching the cortex and the corticomedullary junction between 20 and 23 gw. Few nerve fibers were seen in the medulla in close association with Hassall's corpuscles. In human fetal spleen, NSE-, NF-, S100-, PGP9.5-, and TH-positive nerve fibers were localized in the connective tissue surrounding the splenic artery at 18 gw. Perivascular NSE-, NF-, S100-, PGP9.5-, and TH-positive nerve fibers were seen extending into the white pulp, mainly in association with the central artery and its branches, increasing in density during gestation. Scattered NSE-, NF-, S100-, PGP9.5-, and THpositive nerve fibers and endings were localized in the red pulp from 18 gw onward. The predominant perivascular distribution of most parenchymal nerves implies that thymic and splenic innervation may play an important functional role during intrauterine life. (J Histochem Cytochem 55:813-820, 2007)

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Section: Discussionsupporting

confidence: 92%

Ontogeny of Intrinsic Innervation in the Human Thymus and Spleen

Anagnostou

Doussis-Anagnostopoulou

Tiniakos

et al. 2007

J Histochem Cytochem.

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“…60) Changes in the electrical properties of RBL-2H3 cell membranes upon contact were also noted, and RBL-2H3 cells stopped dividing upon contact and increased their mediator content and number of granules. 61,62) Interactions between Nerves and RBL Cells We have shown direct communication between RBL-2H3 cells and SCG in cocultures using Ca 2ϩ imaging to indicate evidence for activation and signaling. The addition of bradykinin or scorpion venom to SCG cultures only resulted in a dose-dependent neurite activation but did not evoke an increase in [Ca 2ϩ ] i when added directly to RBL-2H3 cells in the absence of SCG neurites.…”

Section: Interaction With Neuronsmentioning

confidence: 93%

Live Cell Imaging to Study Signaling Molecules in Allergic Reactions

Furuno

Nakanishi

2005

Biological & Pharmaceutical Bulletin

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Mast cells are widely distributed throughout the body, predominantly near blood vessels and nerves, and express effector functions in allergic reactions, inflammatory diseases, and host defense. The activation of mast cells results in secretion of the preformed chemical mediators in their granules by a regulated process of exocytosis and leads to synthesis and secretion of lipid mediators and cytokines. Their soluble factors contribute to allergic inflammation. Mast cells are associated with hypersensitivity reactions, not only in the classical immunoglobulin E (IgE)-dependent mechanism but also in an IgE-independent manner. In particular, investigations of potential anatomical and functional interactions between mast cells and the nervous system have recently attracted great interest. To understand these molecular mechanisms in mast cell activation, the ability to visualize, track, and quantify molecules and events in living mast cells is an essential and powerful tool. Recent dramatic advances in imaging technology and labeling techniques have enabled us to carry out these tasks with high spatiotemporal resolution using confocal laser scanning microscopes, green fluorescent protein and its derivatives, and image analysis systems. Here we review our investigations of the dynamic processes of intracellular signaling molecules, cellular structure, and interactions with neurons in mast cells to provide basic and valuable information for allergy and clinical immunology using these new imaging methods.

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“…Actually it was shown that contact with nerve membrane might be a developmental cue leading to maturation of mast cells without changing their phenotype. 16) We demonstrated here that the association with SCG neurites enhanced the response to antigen in BMMCs through the calcium responses and the increase in expression of FceRI (a subunit). This suggested that the mast cells associated with the neurites were able to not only be activated by substance P released from the neurites but also to respond to antigen even if the concentration of IgE against specific antigen was lower.…”

Section: Discussionmentioning

confidence: 77%