Sympathetic co‐transmission to the cauda epididymis of the rat: characterization of postjunctional adrenoceptors and purinoceptors

Ventura, Sabatino; Pennefather, Jocelyn N.

doi:10.1111/j.1476-5381.1991.tb12207.x

Cited by 44 publications

(29 citation statements)

References 14 publications

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“…The latter is consistent with the findings in rats (Ventura & Pennefather 1991, 1994, Chaturapanich et al 2002 and guinea pigs (Haynes & Hill 1996), whereas in the proximal cauda of rats, both a 1 -and a 2 -ADRAs mediate contraction (Chaturapanich et al 2002). Postjunctional ADRA2 have also been found in the cauda epididymidis of guinea pigs; however, they appear to only potentiate the ADRA1-mediated contractile responses (Haynes & Hill 1996).…”

Section: Regional Effects Of Nasupporting

confidence: 80%

“…Predominantly, noradrenergic output stimulates epididymal muscle activity and emission of sperm during ejaculation (Pholpramool et al 1984, Billups et al 1990, Kempinas et al 1998. The effects of noradrenaline (NA) seem to be primarily mediated not only by postjunctional a 1 -adrenoceptors (ADRA; Pholpramool et al 1984, Ventura & Pennefather 1991, Chaturapanich et al 2002, but also by a 2 -ADRA (Haynes & Hill 1996, Chaturapanich et al 2002.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Differential modulation of bovine epididymal activity by oxytocin and noradrenaline

Mewe¹,

Wulfsen²,

Middendorff³

et al. 2007

Reproduction

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Passage of spermatozoa through the epididymis and emission of sperm during ejaculation are based on spontaneous and induced contractions of epididymal peritubular muscle layers. This study deals with the ejaculation-relevant factors noradrenaline (NA) and oxytocin (OT) and their contractile effects in the course of the bovine epididymal duct. Muscle tension recording revealed excitatory effects of NA in all duct regions. A peculiarity was found in a duct section between the mid-cauda and ductus deferens, where the responsiveness to NA was particularly faint in comparison with the adjacent regions. NA-induced contraction was primarily mediated by postjunctional a 2 -adrenoceptors (ADRA) in the caput and corpus regions, and by a 1 -ADRA in the cauda region. Contrary to NA, OT exerted regionally varying effects. The peptide induced contraction in intact and epithelium-denuded caput as well as in epithelium-denuded corpus segments but had a relaxant net effect in intact corpus and proximal cauda segments. Within the mid-cauda, OT evoked strong contraction, which progressively decreased distally. Receptor specificity of the epididymal OT effects was verified using the selective OT receptor (

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Section: Regional Effects Of Nasupporting

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Differential modulation of bovine epididymal activity by oxytocin and noradrenaline

Mewe¹,

Wulfsen²,

Middendorff³

et al. 2007

Reproduction

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“…Furthermore, histochemical studies have shown that ramifications of the cholinergic and adrenergic peritubular nerves surround the smooth muscle cells in the tubular wall of the epididymis and extend into the tubular epithelium (El-Badawi and Schenk 1967). mAChR agonists induce epididymal contraction (Hib 1976;Laitinen and Talo 1981;Pholpramool and Triphrom 1984;Ventura et al 1989), and the mAChR non-selective antagonist, atropine, but not pirenzepine (M 1 -selective antagonist) decreases electrically evoked contractions in the cauda of the epididymis as previously reported (Ventura and Pennefather 1991).…”

Section: Discussionmentioning

confidence: 57%

Expression and localization of muscarinic acetylcholine receptor subtypes in rat efferent ductules and epididymis

et al. 2005

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The expression of muscarinic acetylcholine receptor (mAChR) subtypes (M(1)-M(5)) was studied in the rat efferent ductules and epididymis at the mRNA and protein levels. The relative abundance of each mAChR transcript subtype differed depending on the tissue and the epididymal region analyzed. The M(1) mAChR mRNA level was more abundant in the efferent ductules than in the caput and cauda of the epididymis. The M(2) mAChR mRNA level was similar between the efferent ductules and caput of the epididymis and higher in the cauda region. The M(3) mAChR mRNA level was low in the efferent ductules and caput of the epididymis, but high levels were detected in the cauda region. mRNAs for M(4) and M(5) mAChRs were not detected in these tissues. Our studies indicated a variable degree of immunostaining for each mAChR subtype in a cell-type and tissue-specific pattern. M(1) mAChR was detected over the efferent ductule epithelium. M(2) and M(3) mAChRs were observed in the apical region of the ciliated cells. Apical and narrow cells of the initial segment showed distinct staining by M(1) antibody, whereas a supranuclear reaction was noted in the principal cells of the caput of the epididymis. In addition, staining for M(1) and M(2) mAChRs was visible in the apical membrane of some epithelial cells of the cauda region. M(3) mAChR was detected in the peritubular smooth muscle of the efferent ductules and epididymis. Functional studies suggested the involvement of this subtype in epididymal tubule contraction. Thus, the cell-specific expression of the various mAChR subtypes in the efferent ductules and epididymis suggests that these receptors play a role in the modulation of luminal fluid composition and smooth muscle contraction.

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“…Alhave been widely used to investigate peripheral adrenergic though recent studies have demonstrated that, in the rat, the mechanisms (Saxena, 1970;Swedin, 1971;Anstey & Birming-cauda epididymis contains postjunctional a,-, but not OC2-ham, 1978;1980;Fukushi et al, 1988;Eltze & Boer, 1992; adrenoceptors (Ventura & Pennefather, 1991;, there is no Bultman et al, 1994). Many studies have demonstrated that, in evidence indicating which a-adrenoceptor subtypes mediate the rat, this tissue contains a functionally predominant popucontractile responses of the cauda epididymis of the guinealation of OCIA-adrenoceptors (Mallard et al, 1992;Ohmura et pig.…”

Section: Introductionmentioning

confidence: 99%

α‐Adrenoceptor mediated responses of the cauda epididymis of the guinea‐pig

Haynes

Hill

1996

British J Pharmacology

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1 The subtypes of Lx-adrenoceptor mediating the contractile responses of the cauda epididymis of the guinea-pig were investigated. The ocx-adrenoceptor agonist phenylephrine, but not the LX2-adrenoceptor agonist, xylazine (up to 10 gM), elicited concentration-dependent contractions from preparations of cauda epididymis (EC50 3.4 gM). The L-type Ca2+ channel antagonist, nifedipine (10 gM), reduced the maximal response to phenylephrine (by 77%). Preincubation of tissues with the alB-adrenoceptoralkylating agent, chloroethylclonidine (50 juM, 30 min), shifted phenylephrine concentration-response curves to the right (4 fold) only when the a2-adrenoceptor antagonist idazoxan (100 nM) was included during the pre-incubation with chloroethylclonidine. 2 Xylazine (1 /IM) significantly shifted phenylephrine concentration-response curves to the left (3 fold); this effect was attenuated by idazoxan (100 nM). Both the incubation of preparations with nifedipine (10 gM) and the pre-incubation of preparations with chloroethylclonidine (50 gM, 30 min) attenuated the potentiating effects of xylazine (1 gM). Protection of a2-adrenoceptors with idazoxan (100 nM) during the chloroethylclonidine (50 gM, 30 min) incubation restored the xylazine-mediated enhancement of phenylephrine concentration-response curves. Pertussis toxin (200 ng ml-1, 24 h) attenuated the xylazine (1 gM)-mediated potentiation of phenylephrine concentration-response curves.3 Following the pre-incubation of preparations with chloroethylclonidine (50 gM, 30 min) 5-methylurapidil (10 nM to 3 gM) shifted phenylephrine concentration-response curves, in parallel, to the right with mean pKB values in the range of 8.27 (at 10 nM 5-methylurapidil) to 7.76 (at 3 gM 5-methylurapidil), the addition of idazoxan (100 nM) to the incubation medium did not significantly affect the 5-methylurapidil (10 to 300 nM) pKB values (8.41 to 7.64, respectively). In the presence of both idazoxan (100 nM) and nifedipine (10 pM), and following the pre-incubation with chloroethylclonidine (50 gM, 30 min), 5-methylurapidil (30 to 300 nM) still shifted phenylephrine concentration-response curves to the right (pKB values 7.77 to 7.36, respectively).

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Sympathetic co‐transmission to the cauda epididymis of the rat: characterization of postjunctional adrenoceptors and purinoceptors

Cited by 44 publications

References 14 publications

Differential modulation of bovine epididymal activity by oxytocin and noradrenaline

Differential modulation of bovine epididymal activity by oxytocin and noradrenaline

Expression and localization of muscarinic acetylcholine receptor subtypes in rat efferent ductules and epididymis

α‐Adrenoceptor mediated responses of the cauda epididymis of the guinea‐pig

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