Symbiotic propagation of Dendrobium bigibbum Lindl. with selected saprophytic Basidiomycota

Abstract

Abstract. Bautista NS, Valentino MJG. 2023. Symbiotic propagation of Dendrobium bigibbum Lindl. with selected saprophytic Basidiomycota. Biodiversitas 24: 3519-3527. Compatible mycorrhizal association of orchids with fungi is required for the germination and seedling development. In the present study, the mycorrhizal association of Dendrobium bigibbum with three species of saprophytic Basidiomycota, in particular, Volvariella volvacea, Lentinus tigrinus, and Pleurotus florida were evaluated. Co-culture technique was carried out during rhizoid and seedling development of D. bigibbum. Results revealed that the growth of D. bigibbum in rhizoid and seedling stages was enhanced when symbiotically grown with V. volvacea and L. tigrinus. D. bigibbum grown with V. volvacea had the highest mean length of the 1st leaf during the rhizoid stage. In seedling stage, V. volvacea and L. tigrinus significantly increased the growth of D. bigibbum in terms of length of 2nd leaf and fresh weight of the seedlings. For the length of root, increase in mean length was observed only in D. bigibbum co-cultured with L. tigrinus. In addition, the presence of peloton colonization was observed both in rhizoid and seedling stages. Thus, both V. volvacea and L. tigrinus formed compatible mycorrhizal association with D. bigibbum.

Orchid Mycorrhizal Association of Cultivated Dendrobium Hybrid and Their Role in Seed Germination and Seedling Growth

“…Although these wood-decay fungi could be mycorrhizal, further experiments, such as symbiotic culture, are needed to reach a definitive conclusion. As the use of wood-decay fungi, such as Pleurotus ostreatus and Lentinula edodes , significantly improves the survival and growth of Cattleya hybrid seedlings [ 51 , 52 ], wood-decay fungi may have potential as growth promoters. In addition to wood-decay fungi, HTS identified seven Ceratobasidiaceae OTUs that were not detected by Sanger sequencing.…”