Symbiotic efficiency and genetic diversity of soybean bradyrhizobia in Brazilian soils

Ribeiro, Paula Rose de Almeida; Santos, Jessé Valentim dos; Costa, Elaine Martins da; Lebbe, Luc; Assis, Emanuaelly Silva; Louzada, Marina Oliveira; Guimarães, Amanda Azarias; Willems, Anne

doi:10.1016/j.agee.2015.06.017

Cited by 32 publications

(14 citation statements)

References 58 publications

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“…and showed high similarity to UFLA 06-27 (Bradyrhizobium sp. ), isolated from an area with a history of soybean cultivation and of use of a commercial inoculant (Ribeiro et al, 2015). The UFLA 06-52 showed high similarity with the strain B. elkanii (USDA 76 T ).…”

Section: Identification Of Isolates By 16s Rrna Partial Gene Sequencingmentioning

confidence: 98%

Symbiotic Efficiency and Genotypic Characterization of Variants of Bradyrhizobium spp. in Commercial Inoculants for Soybeans

Barbosa

Costa

Ribeiro

et al. 2017

Rev. Bras. Ciênc. Solo

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High yields obtained from soybean [Glycine max (L.) Merrill], and consequent profits, are related to efficient symbiosis of soybean with nitrogen fixing bacteria of the Bradyrhizobium genus. Inoculation with strains belonging to the Bradyrhizobium japonicum, B. elkanii, and B. diazofficiens species, approved by the Brazilian Ministry of Agriculture (MAPA), is a common practice for this crop. However, variations in the composition of inoculants can change the quality of the product. The aim of this study was to genetically identify and to evaluate the symbiotic efficiency of isolates with cultural characteristics different from those of strains approved by MAPA and which are present in two commercial inoculants for soybean. Isolates obtained from inoculants and the strains SEMIA 5079 (CPAC 15), SEMIA 5080 (CPAC 7), SEMIA 587 (BR 96), and SEMIA 5019 (BR 29), indicated in the formulation of these inoculants, were tested for symbiotic efficiency in soybeans (cultivar: COODETEC 5G 830 RR) in a greenhouse. Identification by partial sequencing of the 16S rRNA gene, together with BOX-PCR analysis, showed that variant isolates were not similar to the inoculant strains indicated in the commercial products, despite belonging to the same genus and/or species. No loss was observed in the nodulation ability of the isolates; however, significant differences were reported in the symbiotic efficiency of the isolates compared to the inoculant strains approved by MAPA. Nevertheless, the presence of morphological variants did not influence the efficiency of inoculant products.

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Section: Identification Of Isolates By 16s Rrna Partial Gene Sequencingmentioning

confidence: 98%

Symbiotic Efficiency and Genotypic Characterization of Variants of Bradyrhizobium spp. in Commercial Inoculants for Soybeans

Barbosa

Costa

Ribeiro

et al. 2017

Rev. Bras. Ciênc. Solo

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“…For that purpose, the genomic DNA of each strain was extracted by alkaline lysis (Niemann et al, 1997) after growing in culture medium 79, in accordance with the growth time to reach log phase. Amplification of the 16S rRNA gene followed the method described in Guimarães et al (2012) and Ribeiro et al (2015) using the primers 27F…”

Section: Phenotypic and Genotypic Characterization Of The Strains Imentioning

confidence: 99%

“…The 16S rRNA gene does not have resolution to discriminate species within some genera (e.g., Bradyrhizobium) (Ribeiro et al, 2015). That is why we refer to strains by their genera, although many strains had 16S rRNA sequences that were 100% similar to type strains of known species (Tables S1 and S2, and Figures S1 and S2).…”

Section: Groups Strainsmentioning

confidence: 99%

“…In addition, it represents genetic resources with potential for biotechnological application, such as plant growthpromoting rhizobacteria (PGPR). These bacteria can be isolated directly from nodules on plants found in the environment under study (Moreira et al, 1993;Moreira et al, 1998;Santos et al, 2017) or indirectly through the use of promiscuous species of trap plants, such as Macroptilium artropurpureum (Borges et al, 2016;Jesus et al, 2005;Lima et al, 2009), Mimosa tenuiflora (Silva et al, 2017), Vigna unguiculata (Castro et al, 2017), Phaseolus vulgaris (Dall'Agnol et al, 2016), Glycine max (Ribeiro et al, 2015), and other species.…”

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confidence: 99%

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Plant growth‐promoting rhizobacterial communities from an area under the influence of iron mining and from the adjacent phytophysiognomies which have high genetic diversity

et al. 2020

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We evaluated symbiotic (rhizobia) and non-symbiotic efficiency and the diversity of native communities of plant growth-promoting rhizobacteria (PGPR) in soil samples from an area in recovery after iron mining (RA) and from adjacent phytophysiognomies (Ironstone Outcrops, Neotropical Savanna, Atlantic Forest) in the Quadrilátero Ferrífero region, MG, Brazil, in two climate seasons. Greenhouse experiments were conducted with siratro (Macroptilium atropurpureum) as a trap plant. The SPAD index in siratro was more effective in detecting the difference between the symbiotic efficiencies of the communities of PGPR than shoot dry matter, with readings of the SPAD index greater in RA. Genetic diversity, of 327 strains evaluated by partial sequencing of the 16S rRNA gene, showed 29 genera in the dry season and 19 in the rainy season, among these genera some recognized as nodulating and endophytes, and others potentially endophytes. Genera belonging to the phyla Acidobacteria, Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria were found. The nodule-forming genera Bradyrhizobium and Rhizobium and the endophytic Paenibacillus were those of highest occurrence in both seasons and in all phytophysiognomies. The greater symbiotic efficiency and the high genetic diversity of the rhizobacterial community found in RA indicate that soil rehabilitation is effectively in process. As most of native legume species form symbiosis with Rhizobium and mainly Bradyrhizobium, these bacterial communities are an important source of potential inoculant strains, as well as, a relevant feature for the successful establishment of diverse legumes for revegetation purposes.

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“…Numerous studies in Africa have shown the presence of effective rhizobia strains among indigenous rhizobia populations (Chibeba et al, 2017;de Almeida Ribeiro et al, 2015;Musiyiwa et al, 2005). In addition, indigenous rhizobia have been described by many studies as being persistent, well adapted to local conditions and therefore can compete successfully at the expense of exotic strains for nodule occupancy and N fixation (Fening and Danso, 2002).…”

Section: Introductionmentioning

confidence: 99%

Indigenous rhizobia strains: The silver bullet for enhanced biological nitrogen fixation and soybean (Glycine max (L.) Merr. ) yield under different soil conditions in South Kivu province, Democratic Republic of Congo

Nabintu¹,

Ndemo²,

Sharwasi³

et al. 2019

Afr. J. Agric. Res.

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Soybean is an important crop in the Democratic Republic of Congo, a country faced with high levels of war induced malnutrition but its productivity is limited by poor soil fertility coupled with low access to nitrogen mineral fertilizers. Commercial rhizobia strains introduced in 2010 failed to adapt and increase soybeans yields at desired level. We studied the performance of six indigenous rhizobia strains in enhancing soybean productivity compared to two commercial strains USDA110 and SEMIA5019. The study was carried out in the greenhouse and field of Kalambo station of International Institute of Tropical Agriculture (IITA), D. R. Congo during 2016/2017 cropping season. The treatments included: (1) N-, control without inoculation and N-fertilizer; (2) N+, non-inoculated control with 80 kg of N ha −1 ; and inoculated with (3) commercial strain Bradyrhizobium diazoefficiens USDA110; (4) commercial strain Bradyrhizobium elkanii SEMIA5019; (5) local strains Bradyrhizobium japonicum NAC17; (6) NAC22; (7) NAC37, (8) NAC42 (9) NAC 46 and (10) NAC78. Greenhouse and field experiments were laid out as completely randomized design and randomized complete block design respectively. The best inoculation treatments across all experiments were the indigenous strains NAC46 and NAC17 which nodulated equally or better than the commercial strain USDA 110. In the field NAC46 and NAC17 increased soybean grain yield from 2.4 to 3.3 t ha-1 and 3.4 t ha-1 ; indicating the increase of 68.7 and 70.8% respectively, over the commercial strain USDA110. The results demonstrated that indigenous rhizobia NAC46 and NAC17 would thus be the silver bullet to enhanced BNF and soybean yields in South Kivu province of Democratic Republic of Congo.

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Symbiotic efficiency and genetic diversity of soybean bradyrhizobia in Brazilian soils

Cited by 32 publications

References 58 publications

Symbiotic Efficiency and Genotypic Characterization of Variants of Bradyrhizobium spp. in Commercial Inoculants for Soybeans

Symbiotic Efficiency and Genotypic Characterization of Variants of Bradyrhizobium spp. in Commercial Inoculants for Soybeans

Plant growth‐promoting rhizobacterial communities from an area under the influence of iron mining and from the adjacent phytophysiognomies which have high genetic diversity

Indigenous rhizobia strains: The silver bullet for enhanced biological nitrogen fixation and soybean (Glycine max (L.) Merr. ) yield under different soil conditions in South Kivu province, Democratic Republic of Congo

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