Symbiotic effectiveness of inoculation with <i>Bradyrhizobium</i> isolates on Cowpea (<i>Vigna unguiculata</i> (L.) Walp) varieties

Ayalew, Tewodros; Yoseph, Tarekegn

doi:10.1080/23311932.2020.1845495

Cited by 9 publications

(9 citation statements)

References 15 publications

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“…The strains, after isolation, were subjected to authentication and characterization ( Degefu et al., 2018 ). Furthermore, the symbiotic effectiveness was confirmed using sand culture, before use in field conductions ( Ayalew and Yoseph 2020 ).…”

Section: Methodsmentioning

confidence: 95%

Leaf growth, gas exchange and assimilation performance of cowpea varieties in response to Bradyrhizobium inoculation

Ayalew

Yoseph

Högy

et al. 2022

Heliyon

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Supplying nitrogen to crops through selecting high N fixing legumes and effective inoculant is one of the key strategies to improve crop productivity. However, studies related to the effect of Bradyrhizobial inoculation on leaf growth, its functioning in relation to photosynthesis, and transpiration efficiency (WUE) of cowpea [ Vigna unguiculata (L.) Walp] varieties in the tropics were inadequate. A two-year field experiment was conducted at three sites to evaluate the effect of inoculation on leaf growth, gas exchanges and photosynthetic efficiency of cowpea varieties. The study treatments were composed of four varieties, Keti (IT99K-1122), TVU, Black eye bean, and White wonderer trailing and three levels of inoculation (non-inoculated or inoculated with Bradyrhizobium strains CP-24 or CP-37). Gas exchange was measured on live plants at 67–77 days after sowing, between 8:00 to 11:00 a.m. and 14:00 to 16:00 p.m. Leaf growth parameters (leaf number and leaf area) were measured by destructive sampling, and the yield data was determined by harvesting plants in the three central rows at physiological maturity. Variety TVU performed best in terms of leaf number, photosynthesis rate, and WUE. Whereas, Black eye bean revealed superior performances for leaf area, leaf area index, and stomatal conductance compared with the rest two varieties. The effect of inoculation was significant with 14.0, 23.8, 13.7, and 11.0% advantage in leaf area, leaf area index, net photosynthesis, and WUE, respectively. Moreover, the performance of cowpea of the 2018 cropping season showed a relative advantage over 2019 in terms of leaf number, leaf area, leaf area index, net photosynthesis, and stomatal conductance. Therefore, inoculating cowpea varieties with effective Bradyrhizobium strain can be a viable alternative to enhance growth, gas exchange, photosynthetic efficiency, and grain yield.

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Section: Methodsmentioning

confidence: 95%

Leaf growth, gas exchange and assimilation performance of cowpea varieties in response to Bradyrhizobium inoculation

Ayalew

Yoseph

Högy

et al. 2022

Heliyon

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“…Among the cultivars, Sarinut-2 and Shitaochi varieties showed the highest explained percentages of relative importance among the groundnut varieties ( Figure 3C ). The breeding programs in groundnut and Rhz inoculation technologies have not benefited from important research activities regarding the improvement of symbiotic effectiveness on a large scale in contrast to soybean in Africa (Dakora and Keya, 1997 ; Ayalew and Yoseph, 2020 ) . The aggregated results suggest that combinations between groundnut genotype × improved rhizobia strain × P addition and the local climatic conditions are major factors of yield variation in Ghana.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, new economic opportunities through the development of soybean-based supply chains and the success story of soybean in Latin America have further served as drivers for high interest in soybean cultivation (Goldsmith, 2008 ; Foyer et al, 2019 ). Other grain legumes, in particular, cowpea and groundnut, which played roles in subsistence and system maintenance of the smallholdings, only received little research interest regarding the improvement of symbiotic effectiveness of the crops (Dakora and Keya, 1997 ; Ayalew and Yoseph, 2020 ) . A possible challenge to the development of a more efficient cowpea rhizobia inoculant with an efficacious BNF potential is the low symbiotic specificity of the crop, leading to the association with a range of indigenous tropical rhizobia with a poor nitrogen-fixation ability (Sena et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Grain Legume Yield Responses to Rhizobia Inoculants and Phosphorus Supplementation Under Ghana Soils: A Meta-Synthesis

et al. 2022

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A discrete number of studies have been conducted on the effects of rhizobia (Rhz) inoculants, phosphorus (P) management, and combined application of Rhz and P fertilizer on the enhancement of grain legume yield across soils of Ghana and elsewhere. However, the extent to which the various inoculated Rhz strains, P application, and combined application of Rhz + P studies contribute to improving yield, performed on a comprehensive analysis approach, and profit farmers are yet to be understood. This study reviewed different experimental studies conducted on soybean (Glycine max (L.) Merr.), cowpea (Vigna unguiculata [L.] Walp), and groundnut (Arachis hypogaea [L.]) to which Rhz inoculants, P supplements, or Rhz + P combination were applied to improve the yield in Ghana. Multiple-step search combinations of published articles and multivariate analysis computing approaches were used to assess the effects of Rhz inoculation, P application, or both application of Rhz and P on yield variation. The random forest (RF) regression model was further employed to quantify the relative importance of various predictor variables on yield. The meta-analysis results showed that cowpea exhibited the highest (61.7%) and groundnut (19.8%) the lowest average yield change. The RF regression model revealed that the combined application of Rhz and P fertilizer (10.5%) and Rhz inoculation alone (7.8%) were the highest explanatory variables to predict yield variation in soybean. The Rhz + P combination, Rhz inoculation, and genotype wang-Kae explained 11.6, 10.02, and 8.04% of yield variability for cowpea, respectively. The yield in the inoculated plants increased by 1.48-, 1.26-, and 1.16-fold when compared to that in the non-inoculated cowpea plants following inoculation with BR 3299, KNUST 1002, and KNUST 1006 strains, respectively. KNUST 1006 strain exhibited the highest yield increase ratio (1.3-fold) in groundnut plants. Inoculants formulation with a viable concentration of 109 cells g−1 and a minimum inoculum rate of 1.0 × 106 cells seed−1 achieved the highest average yield change for soybean but not for cowpea and groundnut. The meta-analysis calls for prospective studies to investigate the minimum rate of bacterial cells required for optimum inoculation responses in cowpea and groundnut.

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“…Szpunar-Krok et al [14] reported an increase by 439 to 800% and 450 to 514% in the nodule number and dry weight, respectively, due to Bradyrhizobia inoculation in soybean cultivars. Furthermore, other researchers have highlighted the beneficial effect of rhizobial inoculation on the number of nodules and dry mass of various leguminous plants [33][34][35]. The tested varieties also displayed different response for the nodule dry weight when inoculated with different strains.…”

Section: Nodule Number and Dry Weight Plant −1mentioning

confidence: 99%

Unlocking the Potential of Inoculation with Bradyrhizobium for Enhanced Growth and Symbiotic Responses in Soybean Varieties under Controlled Conditions

Beruk,

Yoseph,

Ayalew

2024

Agronomy

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Soybean is a crucial crop for sustainable agriculture development as it forms symbiotic relationships with rhizobia species. The effectiveness of inoculants in symbiosis, however, relies on the compatibility of the strain with a specific legume crop variety. This study assessed the symbiotic efficiency of eight Bradyrhizobium strains (SB-36, SB-37, SD-47, SD-50, SD-51, SD-53, SB-113, and SB-120) with five soybean varieties (Gishama, Awassa-95, Boshe, Hawassa-04, and Jalale) using sand culture. The experiment was arranged in a factorial, completely randomized design with three replicates. Data were collected on plant growth, and symbiotic effectiveness indices and subjected to statistical analysis using R software v4.3.1. The results revealed marked differences (p < 0.001) between the varieties, rhizobial strains, and their combined effects on all traits examined. The Jalale variety inoculated with Bradyrhizobium strains SB-113 and SD-53 produced the highest nodules per plant. When inoculated with SD-53, Awassa-95 demonstrated the highest relative symbiotic effectiveness [129.68%], closely followed by the Boshe variety [128.44%] when inoculated with the same strain. All strains exhibited high relative symbiotic effectiveness (>80%) with Awassa-95 and Boshe varieties. The highest absolute symbiotic effectiveness was observed in the Gishama variety inoculated with the SD-53 strain followed by Boshe and Awassa-95 varieties inoculated with this same strain. Notably, strain SD-53 demonstrated remarkable efficiency with the varieties Gishama, Boshe, and Awassa-95 based on both relative and absolute symbiotic effectiveness indices. Varieties inoculated with the SD-53 strain produced deeper green leaves. This study revealed the importance of Bradyrhizobium inoculation to improve soybean performance, for which the SD-53 strain performed best among the strains considered in the current experiment. Therefore, it is plausible to recommend inoculating soybeans with Bradyrhizobium strain SD-53 with prior field evaluation.

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Symbiotic effectiveness of inoculation with Bradyrhizobium isolates on Cowpea (Vigna unguiculata (L.) Walp) varieties

Cited by 9 publications

References 15 publications

Leaf growth, gas exchange and assimilation performance of cowpea varieties in response to Bradyrhizobium inoculation

Leaf growth, gas exchange and assimilation performance of cowpea varieties in response to Bradyrhizobium inoculation

Grain Legume Yield Responses to Rhizobia Inoculants and Phosphorus Supplementation Under Ghana Soils: A Meta-Synthesis

Unlocking the Potential of Inoculation with Bradyrhizobium for Enhanced Growth and Symbiotic Responses in Soybean Varieties under Controlled Conditions

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