Symbiotic effectiveness and phylogeny of rhizobia isolated from faba bean (Vicia faba L.) in Sichuan hilly areas, China

Xu, Kai; Zou, Lan; Penttinen, Petri; Wang, Ke; Heng, Nan Nan; Zhang, Xiaoping; Chen, Qiang; Zhao, Ke; Chen, Yuan Xue

doi:10.1016/j.syapm.2015.06.009

Cited by 17 publications

(15 citation statements)

References 42 publications

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“…Earlier we isolated faba bean rhizobia from 21 sites covering most of the faba bean growing area in Sichuan [11]. The nodulating strains carried nodC similar to those of strains that nodulate winter type faba bean and field pea [11]. In the present study, we collected 31 rhizobium strains from root nodules of fifteen faba bean cultivars grown on the same field.…”

Section: Discussionmentioning

confidence: 96%

“…The faba beans grown in Sichuan are the winter type. Earlier we isolated faba bean rhizobia from 21 sites covering most of the faba bean growing area in Sichuan [11]. The nodulating strains carried nodC similar to those of strains that nodulate winter type faba bean and field pea [11].…”

Section: Discussionmentioning

confidence: 99%

“…Primer pair P1 and P6 was used for amplification of 16S rRNA gene [11]. IGS was amplified using primer pair pHr (F) and p23SR01(R) [11].…”

Section: Methodsmentioning

confidence: 99%

“…Primer pair P1 and P6 was used for amplification of 16S rRNA gene [11]. IGS was amplified using primer pair pHr (F) and p23SR01(R) [11]. Target sequences were amplified in Bio-RAD MyCycler TM with 20 pmol of each primer pair and 50 ng total DNA as template using a Golden Easy PCR System (TIANGEN, Beijing, China).…”

Section: Methodsmentioning

confidence: 99%

“…Earlier we assessed the diversity of faba bean nodulating rhizobia on 21 sites with different environmental conditions across the Sichuan hilly areas, China [11]. For an insight into local diversity under uniform environmental conditions, and faba bean cultivars introduction experiment was coincidentally performed on one field in Sichuan agricultural university farm in Chengdu, so we collected nodules from fifteen faba bean cultivars grown on the same field in Chengdu, China, isolated nodulating rhizobia, and assessed their N 2 -fixation ability and phylogeny.…”

Section: Introductionmentioning

confidence: 99%

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Genetic Diversity and Symbiotic Efficiency of Nodulating Rhizobia Isolated from Root Nodules of Faba Bean in One Field

et al. 2016

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Thirty-one nodulating rhizobium strains were collected from root nodules of spring and winter type faba bean cultivars grown in micro ecoarea, i.e. the same field in Chengdu plain, China. The symbiotic efficiency and phylogeny of these strains were studied. Effectively nitrogen fixing strains were isolated from both winter type and spring type cultivars. Based on phylogenetic analysis of 16S rRNA gene and concatenated sequence of atpD, glnII and recA genes, the isolates were assigned as Rhizobium anhuiense and a potential new Rhizobium species. The isolates were diverse on symbiosis related gene level, carrying five, four and three variants of nifH, nodC and nodD, respectively. Strains carrying similar gene combinations were trapped by both winter and spring cultivars, disagreeing with the specificity of symbiotic genotypes to reported earlier faba bean ecotypes.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

“…Primer pair P1 and P6 was used for amplification of 16S rRNA gene [11]. IGS was amplified using primer pair pHr (F) and p23SR01(R) [11].…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genetic Diversity and Symbiotic Efficiency of Nodulating Rhizobia Isolated from Root Nodules of Faba Bean in One Field

et al. 2016

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Molecular diversity and phylogeny of indigenous Rhizobium leguminosarum strains associated with Trifolium repens plants in Romania

Efrose

Roșu

Stedel

et al. 2017

Antonie van Leeuwenhoek

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The symbiotic nitrogen fixing legumes play an essential role in sustainable agriculture. White clover (Trifolium repens L.) is one of the most valuable perennial legumes in pastures and meadows of temperate regions. Despite its great agriculture and economic importance, there is no detailed available information on phylogenetic assignation and characterization of rhizobia associated with native white clover plants in South-Eastern Europe. In the present work, the diversity of indigenous white clover rhizobia originating in 11 different natural ecosystems in North-Eastern Romania were assessed by a polyphasic approach. Initial grouping showed that, 73 rhizobial isolates, representing seven distinct phenons were distributed into 12 genotypes, indicating a wide phenotypic and genotypic diversity among the isolates. To clarify their phylogeny, 44 representative strains were used in sequence analysis of 16S rRNA gene and IGS fragments, three housekeeping genes (atpD, glnII and recA) and two symbiosis-related genes (nodA and nifH). Multilocus sequence analysis (MLSA) phylogeny based on concatenated housekeeping genes delineated the clover isolates into five putative genospecies. Despite their diverse chromosomal backgrounds, test strains shared highly similar symbiotic genes closely related to Rhizobium leguminosarum biovar trifolii. Phylogenies inferred from housekeeping genes were incongruent with those of symbiotic genes, probably due to occurrence of lateral transfer events among native strains. This is the first polyphasic taxonomic study to report on the MLSA-based phylogenetic diversity of indigenous rhizobia nodulating white clover plants grown in various soil types in South-Eastern Europe. Our results provide valuable taxonomic data on native clover rhizobia and may increase the pool of genetic material to be used as biofertilizers.

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Production of a high molecular weight levan by Bacillus paralicheniformis, an industrially and agriculturally important isolate from the buffalo grass rhizosphere

Nasir

Ahmad

Sattar

et al. 2022

Antonie van Leeuwenhoek

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Symbiotic effectiveness and phylogeny of rhizobia isolated from faba bean (Vicia faba L.) in Sichuan hilly areas, China

Cited by 17 publications

References 42 publications

Genetic Diversity and Symbiotic Efficiency of Nodulating Rhizobia Isolated from Root Nodules of Faba Bean in One Field

Genetic Diversity and Symbiotic Efficiency of Nodulating Rhizobia Isolated from Root Nodules of Faba Bean in One Field

Molecular diversity and phylogeny of indigenous Rhizobium leguminosarum strains associated with Trifolium repens plants in Romania

Production of a high molecular weight levan by Bacillus paralicheniformis, an industrially and agriculturally important isolate from the buffalo grass rhizosphere

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