Symbiotic diversity of Ensifer meliloti strains recovered from various legume species in Tunisia

Mnasri, Bacem; Badri, Y.; Saïdi, Sabrine; Lajudie, Philippe de; Mhamdi, Ridha

doi:10.1016/j.syapm.2009.07.007

Cited by 29 publications

(14 citation statements)

References 31 publications

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“…E. meliloti, a Gram-negative nitrogen-fixing proteobacterium that is distributed worldwide in temperate soils both in free-living and symbiotic form, is considered a model bacterium for legume-rhizobium symbiosis (McIntosh et al 2009). E. meliloti specifically establishes symbiosis with species belonging to three genera of leguminous plants (Melilotus, Medicago, Trigonella), but can be also found on other host species (Mnasri et al 2009). In the early stages of symbiosis plant roots release a cocktail of nodulation-inducing molecules composed predominantly by flavonoids (Peck et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Effect of the plant flavonoid luteolin on Ensifer meliloti 3001 phenotypic responses

et al. 2015

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Background and aims The establishment of a successful symbiosis between the nitrogen-fixing bacterium Ensifer meliloti and compatible host legumes (Medicago spp.) depends on a complex molecular signal exchange. The early stage of signaling involves the release from plant roots of the flavonoid luteolin, which in turn induces the expression of rhizobia nodulation (nod) genes required for root infection and nodule development. To date, the bacterial response to the luteolin perception has been characterized in detail as far as gene expression is concerned. Nevertheless, despite this molecular information, a global view on E. meliloti phenotypes affected by the plant signal luteolin is still lacking.Therefore, an extensive phenotypic investigation of luteolin effect on the nitrogen-fixing E. meliloti 3001 has been performed. Methods A thousand different growth conditions, including sensitivity to osmolites, pH stresses, antibiotics and toxic compounds, were tested by the application of the high-throughput Phenotype MicroArray (PM) technology, as well as by several dedicated assays to evaluate growth stimulation, motility, biofilm formation, Nacyl homoserine lactones and Indole-3-acetic acid (IAA) production. Results Results revealed that the plant signal luteolin affected a wide spectrum of E. meliloti 3001 phenotypes. E. meliloti 3001 displayed an enhanced resistance phenotype in the presence of luteolin toward a broad set of chemicals including several antibiotics, toxic ions, respiration inhibitors, membrane damagers, DNA intercalants and other potential antimicrobial agents. Moreover, the presence of luteolin significantly reduced overall AHLs production, as well as the lag phase in relation to the starting cellular density, the motility and biofilm formation under nutrient-limited growth conditions. An effect on E. meliloti indole-3-acetic acid (IAA) production was also detected in vitro as a response to luteolin. Conclusions Overall, these findings suggest that the plant signal luteolin triggers a broad response in E. meliloti 3001, which was shown to be dependent on nutritional conditions sensed by the bacterium, pointing out a wide role in modifying rhizobial phenotypes, possibly in relation to plant root association and then symbiotic interaction.Plant Soil (2016) 399:159-178

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Section: Introductionmentioning

confidence: 99%

Effect of the plant flavonoid luteolin on Ensifer meliloti 3001 phenotypic responses

et al. 2015

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“…nodulating rhizobia from different geographical areas throughout the world (Biondi et al 2003;Silva et al 2007;Langer et al 2008;Mnasri et al 2009;Elboutahiri et al 2010). However, the present study is the first comprehensive survey of the alfalfa rhizobia native populations covering Serbian territory.…”

Section: Discussionmentioning

confidence: 94%

“…We used (GTG) 5 -PCR genomic fingerprinting, a kind of rep-PCR typing technique appropriate for evaluating strain level/ intraspecies level diversity of rhizobia (Nick et al 1999;Kaksonen et al 2006;Blanco et al 2008). In our hands, this method has proven to be highly discriminative, enabling identification of genetic diversity at the intraspecies level, although it has been less widely used in rhizobial diversity studies than other rep-PCR genomic fingerprinting methods including ERIC-PCR and BOX-PCR (De Bruijn, 1992;Niemann et al 1997;Talebi et al 2008;Mnasri et al 2009;Bizarro et al 2011). In the rep-PCR dendrogram (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, due to its N 2 -fixing symbiosis with rhizobia and the ability to tolerate heavy metals, this legume could play an important role in the restoration of N-depleted soils and might be used as a priority pioneer species for the rehabilitation of degraded and contaminated soils (Gardea-Torresdey et al 1999;Gueye et al 2009). Rhizobia associated with alfalfa have been studied intensively and are included in many genetic and taxonomic studies worldwide (Carelli et al 2000;Biondi et al 2003;Mnasri et al 2009;Saidi et al 2009). It has been discovered, so far, that two species can nodulate promiscuous M. sativa: very well-known S. meliloti and S. medicae (De Lajudie et al 1994;Rome et al 1996).…”

Section: Introductionmentioning

confidence: 99%

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Genetic diversity of rhizobia associated with alfalfa in Serbian soils

et al. 2011

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We have evaluated the genetic diversity and phylogeny of alfalfa rhizobia, originating from different types of soils in Serbia and their ability to establish an effective symbiosis with alfalfa (Medicago sativa L.). A collection of 65 strains isolated from root nodules of alfalfa were characterized by rep-PCR analysis, partial and complete 16S rDNA gene and recA gene sequencing, as well as atpD gene sequencing and DNA-DNA hybridizations. The results of the sequence analyses revealed that Sinorhizobium meliloti is the dominant species in alfalfa nodules. Only one strain was identified as Sinorhizobium medicae, two strains as Rhizobium tibeticum and one strain as Rhizobium sp. Despite the fact that the majority of strains were identified as S. meliloti, a high genetic diversity at strain level was detected. Almost all isolates shared the ability to nodulate and fix nitrogen with M. sativa, except 11 of them, which were incapable of fixing nitrogen with this species. About 50% of the isolates showed values of symbiotic effectiveness (SE) above 50%, while 10% of the strains were highly effective with SE values above 70%. Some of the strains which were highly effective in nitrogen fixation at the same time could intensively solubilize phosphates, offering a possibility for multipurpose inoculum development. This was the first genetic study of rhizobia isolated from this region and also the first report of natural presence of R. tibeticum in root nodules of M. sativa

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“…Lucerne is known to form symbioses with Ensifer meliloti and its sister strain E. medicae (Bailly et al, 2011, 2006; Frame et al, 1998; Frame et al, 1998; Galibert et al, 2001; Jones et al, 2007; Mnasri et al, 2009). …”

Section: Introductionmentioning

confidence: 99%

Is there sufficient Ensifer and Rhizobium species diversity in UK farmland soils to support red clover (Trifolium pratense), white clover (T. repens), lucerne (Medicago sativa) and black medic (M. lupulina)?

Roberts

Jackson

Mauchline

et al. 2017

Applied Soil Ecology

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Symbiotic diversity of Ensifer meliloti strains recovered from various legume species in Tunisia

Cited by 29 publications

References 31 publications

Effect of the plant flavonoid luteolin on Ensifer meliloti 3001 phenotypic responses

Effect of the plant flavonoid luteolin on Ensifer meliloti 3001 phenotypic responses

Genetic diversity of rhizobia associated with alfalfa in Serbian soils

Is there sufficient Ensifer and Rhizobium species diversity in UK farmland soils to support red clover (Trifolium pratense), white clover (T. repens), lucerne (Medicago sativa) and black medic (M. lupulina)?

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