Symbiotic Activity of Pea (Pisum sativum) after Application of Nod Factors under Field Conditions

Siczek, Anna; Lipiec, Jerzy; Wielbo, Jerzy; Kidaj, Dominika; Szarlip, Paweł

doi:10.3390/ijms15057344

Cited by 9 publications

(7 citation statements)

References 27 publications

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“…A similar conclusion may be drawn from the present study. With reference to the long-term average (572 mm) (Siczek et al, 2014), the yearly precipitation of 2015 was similar (531 mm) as measured by the meteorological station close to the experimental field. However, the amount of rainfall during the faba bean growing season (April-August) was considerably lower in 2015 than the long-term average (by 39%).…”

Section: Discussionsupporting

confidence: 61%

“…In a field experiment conducted with pea plants under the same climatic conditions (Siczek et al, 2014), the application of NF increased nitrogenase activity (p < 0.05) and plant growth in a relatively dry season, but this effect was not observed during the wet growing season. A similar conclusion may be drawn from the present study.…”

Section: Discussionmentioning

confidence: 96%

“…The positive effects of NF on pea growth and symbiotic activity were noted in compacted soils (Siczek et al, 2013). In field conditions, NF generally improved nitrogenase activity and pea yield in relatively dry but not during wet growing seasons (Siczek et al, 2014).…”

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confidence: 93%

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Nod factors improve the nitrogen content and rhizobial diversity of faba bean and alter soil dehydrogenase, protease, and acid phosphomonoesterase activities

Siczek¹,

Wielbo²,

Lipiec³

et al. 2019

Int. Agrophys.

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A b s t r a c t. Nod factors produced by rhizobia are one of the most important signals involved in symbiotic associations involving legumes. A field trial was performed to assess the symbiotic activity, rhizosphere biological parameters, and plant biomass of faba bean (Vicia faba L.) treated with Nod factors. The soil was a Haplic Luvisol derived from loess. The faba bean seeds (cv. Granit) were soaked with an Nod factors solution (260 nM) or water (control) and sown. At the flowering stage, the genetic diversity of rhizobia (based on PCR-RFLP profiles and the sequencing of the 16-23S rDNA and nodD gene), nitrogenase activity (acetylene reduction assay), and nodule biomass were evaluated. Nitrogen yield and plant biomass were determined at the flowering and maturity stages. Rhizosphere soil was examined during plant growth in relation to the activities of dehydrogenase, protease, urease, and acid phosphomonoesterase. The results indicated that the application of the Nod factors improved nitrogenase activity (by 74-80%, depending on the parameter analysed) and increased the genetic diversity of rhizobia inhabiting root nodules, plant nitrogen content (by 16.8%, at maturity), and seed protein yield (by 14.6%). The rhizobial population became more heterogeneous under the influence of the Nod factors than it was for the control (12 and 7 specific genotypes, respectively). At the flowering stage, Nod factors enhanced dehydrogenase, protease, and acid phosphomonoesterase activities by 46, 36 and 9%, respectively. The results revealed the positive effect of Nod factors at reducing water deficiency effects during a growing season with a short-term rainfall deficit.

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Section: Discussionsupporting

confidence: 61%

Section: Discussionmentioning

confidence: 96%

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Nod factors improve the nitrogen content and rhizobial diversity of faba bean and alter soil dehydrogenase, protease, and acid phosphomonoesterase activities

Siczek¹,

Wielbo²,

Lipiec³

et al. 2019

Int. Agrophys.

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“…Supplement of pea seeds with Nod factors secreted by Rhizobium leguminosarum bv. viciae strain GR09 before planting resulted in a significant increase in nodule numbers, nitrogenase activity and pea yield under greenhouse and field conditions 4 5 . Treatment with flavonoids such as luteolin or increased flavonoids production in the plant through genetic engineering enhanced both the number of nodules and N 2 fixation of alfalfa 6 .…”

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confidence: 99%

Nodulation Characterization and Proteomic Profiling of Bradyrhizobium liaoningense CCBAU05525 in Response to Water-Soluble Humic Materials

Gao

Jiang

et al. 2015

Sci Rep

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The lignite biodegradation procedure to produce water-soluble humic materials (WSHM) with a Penicillium stain was established by previous studies in our laboratory. This study researched the effects of WSHM on the growth of Bradyrhizobium liaoningense CCBAU05525 and its nodulation on soybean. Results showed that WSHM enhanced the cell density of CCBAU05525 in culture, and increased the nodule number, nodule fresh weight and nitrogenase activity of the inoculated soybean plants. Then the chemical compounds of WSHM were analyzed and flavonoid analogues were identified in WSHM through tetramethyl ammonium hydroxide (TMAH)-py-GC/MS analysis. Protein expression profiles and nod gene expression of CCBAU05525 in response to WSHM or genistein were compared to illustrate the working mechanism of WSHM. The differently expressed proteins in response to WSHM were involved in nitrogen and carbon metabolism, nucleic acid metabolism, signaling, energy production and some transmembrane transports. WSHM was found more effective than genistein in inducing the nod gene expression. These results demonstrated that WSHM stimulated cell metabolism and nutrient transport, which resulted in increased cell density of CCBAU05525 and prepared the bacteria for better bacteroid development. Furthermore, WSHM had similar but superior functions to flavone in inducing nod gene and nitrogen fixation related proteins expression in CCBAU05525.

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“…In this case, it is preferable to use isolated, purified Nod factors in the crop. Applying Nod factors directly to seeds increases the number of root nodules, which are an additional habitat for indigenous rhizobia, and accelerates germination and plant growth (Prithiviraj et al, 2003;Macchiavelli & Belles-Marino, 2004;Kidaj et al, 2011;Podleśny et al, 2014;Siczek et al, 2014). This leads to an increase in the amount of bound nitrogen even at low symbiotic yields of individual strains in the soil population.…”

Section: Nod Factors In Agricultural Practicementioning

confidence: 99%

Biological activity of Nod factors

et al. 2020

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Chemically, the Nod factors (NFs) are lipochitooligosaccharides, produced mainly by bacteria of the Rhizobium genus. They are the main signaling molecules involved in the initiation of symbiosis between rhizobia and legume plants. Nod factors affect plant tissues at very low concentrations, even as low as 10–12 mol/L. They induce root hair deformation, cortical cell division, and root nodules’ formation in the host plant. At the molecular level, the cytoskeleton is reorganized and expression of genes encoding proteins called nodulins is induced in response to Nod factors in the cell. Action of Nod factors is highly specific because it depends on the structure of a particular Nod factor involved, as well as the plant receptor reacting with it.

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Symbiotic Activity of Pea (Pisum sativum) after Application of Nod Factors under Field Conditions

Cited by 9 publications

References 27 publications

Nod factors improve the nitrogen content and rhizobial diversity of faba bean and alter soil dehydrogenase, protease, and acid phosphomonoesterase activities

Nod factors improve the nitrogen content and rhizobial diversity of faba bean and alter soil dehydrogenase, protease, and acid phosphomonoesterase activities

Nodulation Characterization and Proteomic Profiling of Bradyrhizobium liaoningense CCBAU05525 in Response to Water-Soluble Humic Materials

Biological activity of Nod factors

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