Symbiosis between the root-nodule bacterium Sinorhizobium meliloti and alfalfa (Medicago sativa) under salinization conditions

Ibragimova, M. V.; Rumyantseva, M. L.; Онищук, О. П.; Belova, V. S.; Курчак, О. Н.; Andronov, E. E.; Dzyubenko, N. I.; Симаров, Б. В.

doi:10.1134/s0026261706020226

Cited by 10 publications

(10 citation statements)

References 10 publications

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“…In general, these results are not surprising as it is known that salttolerant strains are not necessarily derived from saline soils and vice versa (Zahran, 1999). Ibragimova et al (2006) also found no apparent correlation between salt tolerance and the site of origin or the nodulated plant species in a large collection of Sinorhizobium strains from the salt-polluted area in Aral Sea region. The statistical analysis of the interaction of sites and cultivars of origin in salt tolerance of strains (Table 2) did show some significant difference between groups of strains isolated from 3A, 3B and 4A in comparison with other strains ( Table 2, P-value o 0.0001).…”

Section: Resultsmentioning

confidence: 66%

Diversity ofSinorhizobiumstrains nodulatingMedicago sativafrom different Iranian regions

Talebi¹,

Bahar²,

Saeidi³

et al. 2008

FEMS Microbiology Letters

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Alfalfa is believed to have originated in north-western Iran and has a long history of coexistence with its bacterial symbiont Sinorhizobium in soils of Iran. However, little is known about the diversity of Sinorhizobium strains nodulating Iranian alfalfa genotypes. In this study, Sinorhizobium populations were sampled from eight different Iranian sites using three cultivars of Medicago sativa as trap host plants. A total of 982 rhizobial strains were isolated and species were identified showing a large prevalence of Sinorhizobium meliloti over Sinorhizobium medicae. Analysis of salt tolerance demonstrated a great phenotypic diversity. The genetic diversity of the Sinorhizobium isolates was analysed using BOX-PCR and enterobacterial repetitive intergenic consensus (ERIC)-PCR. Patterns ofBOX-PCR fingerprinting were statistically analysed with AMOVA to evaluate the role of plant variety and site of origin in the genetic variance observed. Results indicated that most of the total molecular variance was attributable to divergence among strains isolated from different sites and cultivars (intrapopulation, strain-by-strain variance). Moreover, the analysis showed the presence of two geographic populations (west and northwest), indicating that the effect of the site of origin could be more relevant in shaping population genetic diversity than the effect of cultivar or individual plant.

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Section: Resultsmentioning

confidence: 66%

Diversity ofSinorhizobiumstrains nodulatingMedicago sativafrom different Iranian regions

Talebi¹,

Bahar²,

Saeidi³

et al. 2008

FEMS Microbiology Letters

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“…Under salt stress conditions, nodulation factors (lipochitooligosaccharides) of rhizobia are communication signals with leguminous plants. So, the root-nodule bacteria grown under these conditions may have specific traits, which enable them to establish a symbiotic interaction [38]. For example, some isolates of S. meliloti recovered from nodules of wild species of alfalfa, meliloti and Trigonella preferably formed a symbiosis with a salt-tolerant legume grown in both salinized and nonsalinized soils [39].…”

Section: Discussionmentioning

confidence: 99%

Alleviation of Salinity Stress Effects in Forage Cowpea (Vigna unguiculata L.) by Bradyrhizobium sp. Inoculation

Omara

El-Gaafarey

2018

MRJI

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To investigate salt stress and inoculation effects on nodulation and growth of forage cowpea (Vigna unguiculata cv. Baladi), experiments were conducted under laboratory, greenhouse and field conditions. Six isolates were tested for salt tolerance by culturing in liquid medium supplemented with 5, 10, 15, 20, 25 and 30 g L-1 NaCl and for their nodulation and N 2-fixation abilities under different soil salinity (1.87, 3.16, 5.88, 9.12 and 12.14 dS m-1), were evaluated, followed by application of single and dual inoculation with the two selected halo-tolerant isolates on the growth dynamics in salt-affected soils (9.12 dS m-1), under normal and saline irrigation water during 2016 and 2017 seasons. In liquid medium supplemented with 30 g L-1 NaCl, results showed that isolate of SARS-Rh5 was the most tolerant to grow compared to the others. In respect to the inoculation, the highest values of plant height (20.26 and 20.73 cm plant-1), dry weight of plant (1.08 and 1.11 g plant-1), dry weight of nodules (0.056 and 0.056 g plant-1), and nitrogen content (22.26 and 23.82 mg plant-1), were Original Research Article content were noticed during the two growing seasons. So that, the inoculation of cowpea plants grown under soil salinity conditions with tolerant Bradyrhizobium is very urgent to help the plant to circumvent the unfavourable conditions.

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“…[2]. In liquid medium, AK23 and HAMBI 1552 tolerate 600 and 750 mmol l À1 NaCl, respectively [10,11], whereas Rm1021 grows poorly on LB agar with 400 mmol l À1 NaCl [12]. The salt tolerance of STM 1064 was estimated by growing the strain in liquid M9 medium with 0, 600, and 800 mmol l À1 NaCl.…”

Section: Methodsmentioning

confidence: 99%

Divergent genes in potential inoculant Sinorhizobium strains are related to DNA replication, recombination, and repair

Penttinen

Greco

Muntyan

et al. 2016

J. Basic Microbiol.

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To serve as inoculants of legumes, nitrogen-fixing rhizobium strains should be competitive and tolerant of diverse environments. We hybridized the genomes of symbiotically efficient and salt tolerant Sinorhizobium inoculant strains onto the Sinorhizobium meliloti Rm1021 microarray. The number of variable genes, that is, divergent or putatively multiplied genes, ranged from 503 to 1556 for S. meliloti AK23, S. meliloti STM 1064 and S. arboris HAMBI 1552. The numbers of divergent genes affiliated with the symbiosis plasmid pSymA and related to DNA replication, recombination and repair were significantly higher than expected. The variation was mainly in the accessory genome, implying that it was important in shaping the adaptability of the strains.

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Symbiosis between the root-nodule bacterium Sinorhizobium meliloti and alfalfa (Medicago sativa) under salinization conditions

Cited by 10 publications

References 10 publications

Diversity ofSinorhizobiumstrains nodulatingMedicago sativafrom different Iranian regions

Diversity ofSinorhizobiumstrains nodulatingMedicago sativafrom different Iranian regions

Alleviation of Salinity Stress Effects in Forage Cowpea (Vigna unguiculata L.) by Bradyrhizobium sp. Inoculation

Divergent genes in potential inoculant Sinorhizobium strains are related to DNA replication, recombination, and repair

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