Symbiont-Mediated Insecticide Detoxification as an Emerging Problem in Insect Pests

Blanton, Alison G.; Peterson, Brittany F.

doi:10.3389/fmicb.2020.547108

Cited by 54 publications

(36 citation statements)

References 71 publications

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“…Given that spraying fenitrothion can enhance the abundance of fenitrothion-degrading Burkholderia strains in Riptortus pedestris gut ( Kikuchi et al, 2012 ), the high level of W. confusa in the migratory locust gut is probably the consequence of the increased amount of pesticides or other stresses affecting the migratory locust. These results further indicated that the sustainable management of major insect pests need to consider the role of gut microbiota ( Blanton and Peterson, 2020 ).…”

Section: Discussionmentioning

confidence: 94%

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Complete Genome Sequence of Weissella confusa LM1 and Comparative Genomic Analysis

et al. 2021

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The genus Weissella is attracting an increasing amount of attention because of its multiple functions and probiotic potential. In particular, the species Weissella confusa is known to have great potential in industrial applications and exhibits numerous biological functions. However, the knowledge on this bacterium in insects is not investigated. Here, we isolated and identified W. confusa as the dominant lactic acid bacteria in the gut of the migratory locust. We named this strain W. confusa LM1, which is the first genome of an insect-derived W. confusa strain with one complete chromosome and one complete plasmid. Among all W. confusa strains, W. confusa LM1 had the largest genome. Its genome was the closest to that of W. confusa 1001271B_151109_G12, a strain from human feces. Our results provided accurate evolutionary relationships of known Weissella species and W. confusa strains. Based on genomic analysis, the pan-genome of W. confusa is in an open state. Most strains of W. confusa had the unique genes, indicating that these strains can adapt to different ecological niches and organisms. However, the variation of strain-specific genes did represent significant correlations with their hosts and ecological niches. These strains were predicted to have low potential to produce secondary metabolites. Furthermore, no antibiotic resistance genes were identified. At the same time, virulence factors associated with toxin production and secretion system were not found, indicating that W. confusa strains were not sufficient to perform virulence. Our study facilitated the discovery of the functions of W. confusa LM1 in locust biology and their potential application to locust management.

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Section: Discussionmentioning

confidence: 94%

“…Some insect pests possessed insecticide resistance for bacteria-mediated degradation ( Itoh et al, 2018 ; Blanton and Peterson, 2020 ). Exploring the dominant gut bacteria and its potential functions will be helpful in pesticide development, pest management strategies, and pesticide bioremediation.…”

Section: Discussionmentioning

confidence: 99%

Complete Genome Sequence of Weissella confusa LM1 and Comparative Genomic Analysis

et al. 2021

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“…In fact, compared with Hamiltonella- free aphid clones, Hamiltonella -infected aphid clones exhibit lower sensitivity to most of the tested insecticides at low concentrations ( 27 ). More generally, although the mechanisms of resistance to insecticides are diversified, detoxification from insecticides mediated by symbionts within insect pests and vectors is a trend that has now become clear and that is configured as an emerging problem in the control of these insects ( 28 ).…”

Section: Resultsmentioning

confidence: 99%

Phylogenomics Reveals thatAsaiaSymbionts from Insects Underwent Convergent Genome Reduction, Preserving an Insecticide-Degrading Gene

Comandatore

Damiani

Cappelli

et al. 2021

mBio

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The mosquito microbiota is composed of several lineages of microorganisms whose ecological roles and evolutionary histories have yet to be investigated in depth. Among these microorganisms, Asaia bacteria play a prominent role, given their abundance in the gut, reproductive organs, and salivary glands of different mosquito species, while their presence has also been reported in several other insects. Notably, Asaia has great potential as a tool for the control of mosquito-borne diseases. Here, we present a wide phylogenomic analysis of Asaia strains isolated from different species of mosquito vectors and from different populations of the Mediterranean fruit fly (medfly), Ceratitis capitata, an insect pest of worldwide economic importance. We show that phylogenetically distant lineages of Asaia experienced independent genome reductions, despite following a common pattern, characterized by the early loss of genes involved in genome stability. This result highlights the role of specific metabolic pathways in the symbiotic relationship between Asaia and the insect host. Finally, we discovered that all but one of the Asaia strains included in the study possess the pyrethroid hydrolase gene. Phylogenetic analysis revealed that this gene is ancestral in Asaia, strongly suggesting that it played a role in the establishment of the symbiotic association between these bacteria and the mosquito hosts. We propose that this gene from the symbiont contributed to initial pyrethroid resistance in insects harboring Asaia, also considering the widespread production of pyrethrins by several plants. IMPORTANCE We have studied genome reduction within several strains of the insect symbiont Asaia isolated from different species/strains of mosquito and medfly. Phylogenetically distant strains of Asaia, despite following a common pattern involving the loss of genes related to genome stability, have undergone independent genome reductions, highlighting the peculiar role of specific metabolic pathways in the symbiotic relationship between Asaia and its host. We also show that the pyrethroid hydrolase gene is present in all the Asaia strains isolated except for the South American malaria vector Anopheles darlingi, for which resistance to pyrethroids has never been reported, suggesting a possible involvement of Asaia in determining resistance to insecticides.

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“…In fact, compared with Hamiltonella-free aphid clones, Hamiltonella- infected aphid clones exhibit lower sensitivity to most of the tested insecticides at low concentrations (27). More generally, although the mechanisms of resistance to insecticides are diversified, detoxification from insecticides mediated by symbionts within insect pests and vectors is a trend that has now become clear, and which is configured as an emerging problem in control of these insects (28).…”

Section: Resultsmentioning

confidence: 99%

Phylogenomics reveals thatAsaiasymbionts from insects underwent convergent genome reduction, preserving an insecticide-degrading gene

Comandatore¹,

Damiani

Cappelli

et al. 2020

Preprint

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The mosquito microbiota is composed by several lineages of microorganisms whose ecological roles and evolutionary histories have yet to be investigated in depth. Among these microorganisms, Asaia bacteria play a prominent role, given its abundance in the gut, reproductive organs and salivary glands of different mosquito species, while its presence has also been reported in several other insects. Notably, Asaia has great potential as a tool for the control of mosquito-borne diseases. Here, we present a wide phylogenomic analysis of Asaia strains isolated from different species of mosquito vectors and from different populations of the Mediterranean fruit fly (medfly) Ceratitis capitata, an insect pest of worldwide economic importance. We show that phylogenetically distant lineages of Asaia experienced independent genome reductions, despite following a common pattern, characterized by the early loss of genes involved in genome stability. This result highlights the role of specific metabolic pathways in the symbiotic relationship between Asaia and the insect host. Finally, we discovered that all but one of the Asaia strains included in the study possess the pyrethroid hydrolase gene. Phylogenetic analysis revealed that this gene is ancestral in Asaia, strongly suggesting that it played a role in the establishment of the symbiotic association between these bacteria and the mosquito hosts.

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Symbiont-Mediated Insecticide Detoxification as an Emerging Problem in Insect Pests

Cited by 54 publications

References 71 publications

Complete Genome Sequence of Weissella confusa LM1 and Comparative Genomic Analysis

Complete Genome Sequence of Weissella confusa LM1 and Comparative Genomic Analysis

Phylogenomics Reveals thatAsaiaSymbionts from Insects Underwent Convergent Genome Reduction, Preserving an Insecticide-Degrading Gene

Phylogenomics reveals thatAsaiasymbionts from insects underwent convergent genome reduction, preserving an insecticide-degrading gene

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