Sydney rock oyster (Saccostrea glomerata) hemocytes: Morphology and function

Aladaileh, Saleem; Nair, Sham V.; Birch, Debra; Raftos, David A.

doi:10.1016/j.jip.2007.02.011

Cited by 129 publications

(102 citation statements)

References 64 publications

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“…Cells were stained with May-Grünwald (5 min), washed in aqua dest (1 ½ min), stained with 5% Giemsa (13 min), followed by a short washing step (1 ½ min in aqua bidest.) according to Aladaileh et al (2007) and optimized Sigma Ò staining protocols.…”

Section: Total/differential Haemocyte Countmentioning

confidence: 99%

“…Three major haemocyte types (granulocytes, hyalinocytes and small basophilic haemocytes) were identified by light microscopy based on nucleus:cytoplasm ration and the presence or absence of cytoplasmic granules (Aladaileh et al, 2007). At least 200 cells were counted under a light microscope (1000Â) to determine the ratio of the three haemocyte types.…”

Section: Total/differential Haemocyte Countmentioning

confidence: 99%

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Effects of various pollutant mixtures on immune responses of the blue mussel (Mytilus edulis) collected at a salinity gradient in Danish coastal waters

Höher

Köhler

Strand

et al. 2012

Marine Environmental Research

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a b s t r a c tThe Baltic Sea is a semi-enclosed sea with a steady salinity gradient (3&e30&). Organisms have adapted to such low salinities, but are suspected to be more susceptible to stress. Within the frame of the integrated environmental monitoring BONUS þ project "BEAST" the applicability of immune responses of the blue mussel was investigated in Danish coastal waters. The sampling sites were characterised by a salinity range (11e19&) and different mixtures of contaminants (metals, PAHs and POPs), according to chemical analysis of mussel tissues. Variation partitioning (redundancy analysis) was applied to decompose salinity and contamination effects. The results indicated that cellular immune responses (total and differential haemocyte count, phagocytic activity and apoptosis) were mainly influenced by contaminants, whereas humoral factors (haemolytic activity) were mainly impacted by salinity. Hence, cellular immune functions may be suitable as biomarkers in monitoring programmes for the Baltic Sea and other geographic regions with salinity variances of the studied range.Crown

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Section: Total/differential Haemocyte Countmentioning

confidence: 99%

Section: Total/differential Haemocyte Countmentioning

confidence: 99%

Effects of various pollutant mixtures on immune responses of the blue mussel (Mytilus edulis) collected at a salinity gradient in Danish coastal waters

Höher

Köhler

Strand

et al. 2012

Marine Environmental Research

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show abstract

“…Resale or republication not permitted without written consent of the publisher Pal 1995, López et al 1997, Pipe et al 1997, Cima et al 2000, Chang et al 2005, Zhang et al 2006, Aladaileh et al 2007, García-García et al 2008. Interspecies variability and methodological differences in specimen collection and processing have been considered the principal reasons for the current lack of consensus on the actual number of distinct haemocytic subpopulations that can be discerned morphologically (see reviews in Cheng 1981, 1984, Auffret 1988, Hine 1999.…”

Section: Abstract: Mya Arenaria · Vibrio Splendidus · Haemocyte Subpmentioning

confidence: 99%

Changes induced by two strains of Vibrio splendidus in haemocyte subpopulations of Mya arenaria, detected by flow cytometry with LysoTracker

Mateo¹,

Spurmanis²,

Siah³

et al. 2009

Dis. Aquat. Org.

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“…In contrast, the oil spill-damaged oysters exhibited more blast-like cells than the control oysters (14.7 vs. 10.7%, respectively). Blast-like cells are very small agranular cells that are considered stem cells freely circulating in the hemolymph (Cima et al 2000;Aladaileh et al 2007;Matozzo et al 2008;Travers et al 2008;Donaghy et al 2010). Although current knowledge about hemopoiesis in marine bivalves is somewhat limited (Hine 1999;Matozzo et al 2008), multiplication of blastlike cells might have been initiated to mainly restore the decreased granulocyte population.…”

Section: Discussionmentioning

confidence: 99%

Hemocyte parameters of the Pacific oyster Crassostrea gigas a year after the Hebei Spirit oil spill off the west coast of Korea

et al. 2010

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In marine bivalves, hemocytes support various physiological functions, including immune defense, nutrient transport, shell repair, and homeostatic maintenance. Although the eVects of marine contaminants on the immunological functions of bivalves have been extensively investigated, the impacts of oil spills are not well understood. Therefore, we investigated hemocyte parameters in the PaciWc oyster Crassostrea gigas 13 months after the Hebei Spirit oil spill (December 2007) oV the west coast of Korea. The parameters studied included hemocyte concentration and mortality, relative proportion of hemocyte populations, and immunological functions such as phagocytosis and oxidative activity using Xow cytometry. These immune-related parameters in oysters damaged by the oil spill were also compared to control oysters that were collected from an area unaVected by the spill. The Xow cytometry study indicated that granulocyte population, phagocytic capacity, and reactive oxygen species production in oysters exposed to crude oil 13 months prior were depressed compared to the unexposed control oysters. Our data suggest that immunocompetence in oysters aVected by the oil spill had not fully recovered 1 year after the accident, although more detailed studies on the physiology and disease resistance should be performed.

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Sydney rock oyster (Saccostrea glomerata) hemocytes: Morphology and function

Cited by 129 publications

References 64 publications

Effects of various pollutant mixtures on immune responses of the blue mussel (Mytilus edulis) collected at a salinity gradient in Danish coastal waters

Effects of various pollutant mixtures on immune responses of the blue mussel (Mytilus edulis) collected at a salinity gradient in Danish coastal waters

Changes induced by two strains of Vibrio splendidus in haemocyte subpopulations of Mya arenaria, detected by flow cytometry with LysoTracker

Hemocyte parameters of the Pacific oyster Crassostrea gigas a year after the Hebei Spirit oil spill off the west coast of Korea

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