Sycophila pistacina (Hymenoptera: Eurytomidae): A valid species

Lotfalizadeh, Hossein; Delvare, Gérard; Rasplus, Jean‐Yves

doi:10.14411/eje.2008.019

Cited by 10 publications

(7 citation statements)

References 26 publications

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“…The ITS2 fragment also usually possesses nucleotide differences between close species pairs (Coleman 2003) and is often an effective tool for species diagnosis (Hackett et al 2000;Hung et al 2004;Ben-David et al 2007). Both COI and ITS2 have been shown to be effective in tracing species boundaries in varied hymenopteran groups (Dowton & Austin 2001;Alvarez & Hoy 2002;Pinto et al 2002;Scheffer & Grissell 2003;Jousselin et al 2004;Jiang et al 2006a,b;Lotfalizadeh et al 2008).…”

Section: Introductionmentioning

confidence: 99%

COI and ITS2 sequences delimit species, reveal cryptic taxa and host specificity of fig‐associated Sycophila (Hymenoptera, Eurytomidae)

Zhou

Feng

et al. 2009

Molecular Ecology Resources

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Although the genus Sycophila has broad host preferences, some species are specifically associated with figs as nonpollinator wasps. Because of their sexual dimorphism, morphological plasticity, cryptic mating behaviour and poorly known biology, species identifications are often uncertain. It is particularly difficult to match conspecific females and males. In this study, we employed two molecular markers, mitochondrial COI and nuclear ITS2, to identify Sycophila from six Chinese fig species. Morphological studies revealed 25 female and male morphs, while sequence results for both genes were consistent in supporting the presence of 15 species, of which 13 were host specialists and two used dual hosts. A single species of Sycophila was respectively found on four fig species, but six species were isolated from Ficus benjamina and a same number was reared from Ficus microcarpa. Sequence results revealed three male morphs in one species and detected two species that were overlooked by morphological analysis.

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Section: Introductionmentioning

confidence: 99%

COI and ITS2 sequences delimit species, reveal cryptic taxa and host specificity of fig‐associated Sycophila (Hymenoptera, Eurytomidae)

Zhou

Feng

et al. 2009

Molecular Ecology Resources

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“…For most of the parasitoids we examined, we amplified a fragment of the 3′ region of COI using the forward primer Jerry (5′-CAACATTTATTTTGATTTTTTGG-3′) and reverse primer Pat (5′-TCCAATGCACTAATCTGCCATATTA-3′) (Ghararieh, Bruford, Dawah, Fernandes, & Dodd, 2006;Simon et al, 1994) with the PCR protocol described in Lotfalizadeh, Delvare, and Rasplus (2008). For most of the parasitoids we examined, we amplified a fragment of the 3′ region of COI using the forward primer Jerry (5′-CAACATTTATTTTGATTTTTTGG-3′) and reverse primer Pat (5′-TCCAATGCACTAATCTGCCATATTA-3′) (Ghararieh, Bruford, Dawah, Fernandes, & Dodd, 2006;Simon et al, 1994) with the PCR protocol described in Lotfalizadeh, Delvare, and Rasplus (2008).…”

Section: Dna Extraction and Amplificationmentioning

confidence: 99%

“…For the mitochondrial gene cytochrome oxidase I (COI) gene, we amplified two nonoverlapping fragments. For most of the parasitoids we examined, we amplified a fragment of the 3′ region of COI using the forward primer Jerry (5′-CAACATTTATTTTGATTTTTTGG-3′) and reverse primer Pat (5′-TCCAATGCACTAATCTGCCATATTA-3′) (Ghararieh, Bruford, Dawah, Fernandes, & Dodd, 2006;Simon et al, 1994) with the PCR protocol described in Lotfalizadeh, Delvare, and Rasplus (2008). In addition, we amplified a fragment that corresponds with the "Barcode" region of COI using a novel forward primer "EurytomidF" (5′-CCWGGKTCWTTAATTGGRAATGATC-3′) and the commonly used barcode reverse primer HCO (Simon et al, 1994) using the PCR protocol described in Hebert, Penton, Burns, Janzen, and Hallwachs (2004).…”

Section: Dna Extraction and Amplificationmentioning

confidence: 99%

Identification of the parasitoid community associated with an outbreaking gall wasp,Zapatella davisae, and their relative abundances in New England and Long Island, New York

Davis

Andersen

Elkinton

2018

Ecology and Evolution

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Gall wasps (Hymenoptera: Cynipidae) are phytophagous insects that often go unnoticed; however, when they are introduced to a new area or released from their natural enemies, they have the capacity to outbreak and cause extensive foliar damage. One such outbreaking pest, Zapatella davisae (Cynipidae: Cynipini), causes significant damage and mortality to black oak, Quercus velutina, in the northeastern United States. In this study, we aimed to identify the parasitoid community associated with Z. davisae, compare differences in percent parasitism of Z. davisae in Cape Cod and Long Island, and determine which parasitoid species contribute most to parasitism in each region. From both locations, we reared parasitoids, identified morphological groups, analyzed percent parasitism rates for each group, and used DNA barcoding to provide species‐level identifications. On Long Island, there was nearly 100% parasitism in 2015 followed by a near total collapse of the population in 2016. In contrast, parasitism rates were lower and remained consistent on Cape Cod between 2015 and 2016, which may explain the greater canopy damage observed in that region. Species of Sycophila were the dominant parasitoids, with one species Sycophila nr. novascotiae representing ~65% of reared parasitoids from Long Island, and two species of Sycophila (S. nr. novascotiae and S. foliatae) with near equal representations on Cape Cod. In order to manage an insect pest, it is important to understand factors that influence its mortality and survival. An understanding of how these infestations progress overtime can help predict the impact that newer infestations in Nantucket, MA, and coastal Rhode Island will have on black oak populations and will aid in the management of this rapidly spreading gall wasp pest.

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“…Mitochondrial cytochrome c oxidase I (COI) and nuclear ribosomal DNA internal transcribed spacer 2 (ITS2) were sequenced to clarify species boundaries and to match genders. Both COI and ITS2 have been shown to effectively identify species of hymenopteran insects (Dowton et al 2001;Alvarez & Hoy 2002;Pinto et al 2002;Scheffer & Grissell 2003;Jousselin et al 2004;Jiang et al 2006a,b;Lotfalizadeh et al 2008;Li et al 2010). One to 11 individuals (depending on availability) of each species as determined by females and each of the distinguishable male morph were sequenced.…”

Section: Dna Extraction Pcr and Sequencingmentioning

confidence: 99%

Molecular approaches identify known species, reveal cryptic species and verify host specificity of Chinese Philotrypesis (Hymenoptera: Pteromalidae)

Zhou

Xiao

Bian

et al. 2012

Molecular Ecology Resources

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Philotrypesis, a major component of the fig wasp community (Hymenoptera: Pteromalidae), is a model taxon for studying male fighting and mating behaviour. Its extreme sexual dimorphism and male polymorphism render species identification uncertain and in-depth research on its ecology, behaviour and other evolutionary topics challenging. The fig wasps' enclosed habitat within the syconia makes their mating behaviour inaccessible, to the extent of matching conspecific females and males. In this study, we combine morphological and molecular analyses to identify species of Philotrypesis sampled from south China and to associate their extraordinarily dimorphic genders and labile male morphologies. Morphological evaluations of females identify 22 species and 28 male morphs. The mitochondrial cytochrome c oxidase I and nuclear internal transcribed spacer 2 data detect 21 species using females, and 15 species among the males. Most of the males match the species as delimited by females. Both markers reveal cryptic species in P. quadrisetosa on Ficus vasculosa. Most species of wasps live on one species of fig but three species co-occur in two hosts (F. microcarpa and F. benjamina), which indicates host switching.

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Sycophila pistacina (Hymenoptera: Eurytomidae): A valid species

Cited by 10 publications

References 26 publications

COI and ITS2 sequences delimit species, reveal cryptic taxa and host specificity of fig‐associated Sycophila (Hymenoptera, Eurytomidae)

COI and ITS2 sequences delimit species, reveal cryptic taxa and host specificity of fig‐associated Sycophila (Hymenoptera, Eurytomidae)

Identification of the parasitoid community associated with an outbreaking gall wasp,Zapatella davisae, and their relative abundances in New England and Long Island, New York

Molecular approaches identify known species, reveal cryptic species and verify host specificity of Chinese Philotrypesis (Hymenoptera: Pteromalidae)

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