Switch in Chemokine Receptor Phenotype on Memory T Cells without a Change in the Cytokine Phenotype

Aarvak, Tanja; Strand, Eivind R; Teigland, Jens; Miossec, Pierre; Natvig, J. B.

doi:10.1046/j.1365-3083.2001.00957.x

Cited by 8 publications

(6 citation statements)

References 37 publications

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“…We found that NP tissues showed excessive accumulation of MBP + cells, CD11c + cells, 2D7 + cells, and HNE + cells compared to UP tissues from CRSsNP or CRSwNP. Although CD11c is not exclusively as marker for dendritic cell, these data are in agreement with previously reported elevated eosinophils, dendritic cells, basophils, and neutrophils in CRSwNP [18]–[22], [23]–[27]. In addition, in non-asthmatic CRS patients, the infiltration of MBP + and CD11c + cells increased gradually with the disease progression (from control to CRSsNP and to CRSsNP).…”

Section: Discussionsupporting

confidence: 92%

“…Specifically, the type 2 innate lymphoid cells which produce IL-5 and IL-13 are responded to exposure of innate cytokines such as IL-25 and IL-33 [14]. In addition, these innate cytokines are produced by innate immune cells including, eosinophils, mast cell, macrophages, dendrite cells, basophils, and neutrophils [15]–[18]. However, to date, the relationship between innate immune cells and pathogenesis of CRS in Asian patients has not yet been investigated.…”

Section: Introductionmentioning

confidence: 99%

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MBP-Positive and CD11c-Positive Cells Are Associated with Different Phenotypes of Korean Patients with Non-Asthmatic Chronic Rhinosinusitis

et al. 2014

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BackgroundAsthmatic nasal polyps primarily exhibit eosinophilic infiltration. However, the identities of the immune cells that infiltrate non-asthmatic nasal polyps remain unclear. Thus, we thought to investigate the distribution of innate immune cells and its clinical relevance in non-asthmatic chronic rhinosinusitis (CRS) in Korea.MethodsTissues from uncinate process (UP) were obtained from controls (n = 18) and CRS without nasal polyps (CRSsNP, n = 45). Nasal polyps (NP) and UP were obtained from CRS with nasal polyps (CRSwNP, n = 56). The innate immune cells was evaluated by immunohistochemistry such as, eosinophil major basic protein (MBP), tryptase, CD68, CD163, CD11c, 2D7, human neutrophil elastase (HNE) and its distribution was analyzed according to clinical parameters.ResultsIn comparisons between UP from each group, CRSwNP had a higher number of MPB+, CD68+, and CD11c+ cells relative to CRSsNP. Comparisons between UP and NP from CRSwNP indicated that NP have a higher infiltrate of MBP+, CD163+, CD11c+, 2D7+ and HNE+ cells, whereas fewer CD68+ cells were found in NP. In addition, MBP+ and CD11c+ cells were increased from UP of CRSsNP, to UP of CRSwNP, and to NP of CRSwNP. Moreover, in UP from CRSwNP, the number of MBP+ and CD11c+ cells positively correlated with CT scores. In the analysis of CRSwNP phenotype, allergic eosinophilic polyps had a higher number of MBP+, tryptase+, CD11c+, 2D7+ cells than others, whereas allergic non-eosinophilic polyps showed mainly infiltration of HNE+ and 2D7+ cells.ConclusionsThe infiltration of MBP+ and CD11c+ innate immune cells show a significant association with phenotype and disease extent of CRS and allergic status also may influences cellular phenotype in non-asthmatic CRSwNP in Korea.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

MBP-Positive and CD11c-Positive Cells Are Associated with Different Phenotypes of Korean Patients with Non-Asthmatic Chronic Rhinosinusitis

et al. 2014

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“…Moreover, it is quite clear that the correlation of a committed functional phenotype with a particular pattern of cell surface chemokine receptors is not absolute. CCR3 can thus be induced on polarized Th1 cells, and CCR5 can be induced on Th2 cells in the presence of an appropriate cytokine milieu (16).…”

mentioning

confidence: 99%

Patterns of Chemokine Receptor Expression on Peripheral Blood γδ T Lymphocytes: Strong Expression of CCR5 Is a Selective Feature of Vδ2/Vγ9 γδ T Cells

Glatzel

Wesch

Schiemann

et al. 2002

The Journal of Immunology

135

137

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γδ T lymphocytes play an important role in the immune defense against infection, based on the unique reactivity of human Vδ2Vγ9 γδ T cells toward bacterial phosphoantigens. Chemokines and their corresponding receptors orchestrate numerous cellular reactions, including leukocyte migration, activation, and degranulation. In this study we investigated the expression of various receptors for inflammatory and homeostatic chemokines on peripheral blood γδ T cells and compared their expression patterns with those on αβ T cells. Although several of the analyzed receptors (including CCR6, CCR7, CXCR4, and CXCR5) were not differentially expressed on γδ vs αβ T cells, γδ T cells expressed strongly increased levels of the RANTES/macrophage inflammatory protein-1α/-1β receptor CCR5 and also enhanced levels of CCR1–3 and CXCR1–3. CCR5 expression was restricted to Vδ2 γδ T cells, while the minor subset of Vδ1 γδ T cells preferentially expressed CXCR1. Stimulation with heat-killed extracts of Mycobacterium tuberculosis down-modulated cell surface expression of CCR5 on γδ T cells in a macrophage-dependent manner, while synthetic phosphoantigen isopentenyl pyrophosphate and CCR5 ligands directly triggered CCR5 down-modulation on γδ T cells. The functionality of chemokine receptors CCR5 and CXCR3 on γδ T cells was demonstrated by Ca2+ mobilization and chemotactic response to the respective chemokines. Our results identify high level expression of CCR5 as a characteristic and selective feature of circulating Vδ2 γδ T cells, which is in line with their suspected function as Th1 effector T cells.

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“…This is in agreement with a meticulous study examining the expression of 11 different chemokine receptors on peripheral blood lymphocytes [ 50 ]. It has been suggested by other groups that T helper subsets (i.e., Th1, Th2, and Th17) [ 51 – 53 ] and cytotoxic T lymphocyte subsets (i.e., Tc1 and Tc2) [ 54 ] have specific chemokine receptor expression patterns, but subsequent studies have shown that the overlap between the subpopulations is substantial and that the chemokine receptor profile can change without a concomitant change in the cytokine profile [ 50 , 55 – 58 ]. Thus, the use of chemokine receptors as markers for T lymphocyte subsets is controversial.…”

Section: Discussionmentioning

confidence: 99%

Colorectal cancer-infiltrating T lymphocytes display a distinct chemokine receptor expression profile

et al. 2017

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BackgroundT lymphocytes exert important homeostatic functions in the healthy intestinal mucosa, whereas in case of colorectal cancer (CRC), infiltration of T lymphocytes into the tumor is crucial for an effective anti-tumor immune response. In both situations, the recruitment mechanisms of T lymphocytes into the tissues are essential for the immunological functions deciding the outcome. The recruitment of T lymphocytes is largely dependent on their expression of various chemokine receptors. The aim of this study was to identify potential chemokine receptors involved in the recruitment of T lymphocytes to normal human colonic mucosa and to CRC tissue, respectively, by examining the expression of 16 different chemokine receptors on T lymphocytes isolated from these tissues.MethodsTissues were collected from patients undergoing bowel resection for CRC. Lymphocytes were isolated through enzymatic tissue degradation of CRC tissue and nearby located unaffected mucosa, respectively. The expression of a broad panel of chemokine receptors on the freshly isolated T lymphocytes was examined by flow cytometry.ResultsIn the normal colonic mucosa, the frequencies of cells expressing CCR2, CCR4, CXCR3, and CXCR6 differed significantly between CD4+ and CD8+ T lymphocytes, suggesting that the molecular mechanisms mediating T lymphocyte recruitment to the gut differ between CD4+ and CD8+ T lymphocytes. In CRC, the frequencies of cells expressing CCR2 and CXCR5 were significantly lower in both the CD4+ and CD8+ T lymphocyte populations compared to unaffected colonic mucosa, and the frequency of CCR9+ cytotoxic T lymphocytes was significantly decreased in CRC tissue.ConclusionsWith regard to the normal gut mucosa, the results suggest that the molecular mechanisms mediating T lymphocyte recruitment differ between CD4+ and CD8+ T lymphocytes, which are important for understanding gut homeostasis. Importantly, T lymphocytes from CRC compared to normal colonic tissue displayed a distinct chemokine receptor expression profile, suggesting that mechanisms for recruitment of T lymphocytes to CRC tissue are skewed compared to normal colonic mucosa. Understanding these mechanisms could help in developing new strategies in cancer immunotherapy and to optimize already available alternatives such as immune checkpoint inhibitors.

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Switch in Chemokine Receptor Phenotype on Memory T Cells without a Change in the Cytokine Phenotype

Cited by 8 publications

References 37 publications

MBP-Positive and CD11c-Positive Cells Are Associated with Different Phenotypes of Korean Patients with Non-Asthmatic Chronic Rhinosinusitis

MBP-Positive and CD11c-Positive Cells Are Associated with Different Phenotypes of Korean Patients with Non-Asthmatic Chronic Rhinosinusitis

Patterns of Chemokine Receptor Expression on Peripheral Blood γδ T Lymphocytes: Strong Expression of CCR5 Is a Selective Feature of Vδ2/Vγ9 γδ T Cells

Colorectal cancer-infiltrating T lymphocytes display a distinct chemokine receptor expression profile

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