Switch from Asexual to Sexual Reproduction in the Planarian Dugesia ryukyuensis

Hoshi, Motonori; Kobayashi, Kazuya; Arioka, Sachiko; Hase, Sumitaka; Matsumoto, Midori

doi:10.1093/icb/43.2.242

Cited by 28 publications

(17 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…S5A) (Gurley et al, 2008;Iglesias et al, 2008;Petersen and Reddien, 2008). Remarkably, we detected high levels of nuclear β-CATENIN-1 in the male gonads (the testis), particularly in the germ cells and in the nucleus of the cells around the ovaries, where female germ cells are found (Hoshi et al, 2003) (Fig. 4A).…”

Section: β-Catenin-1 Is Required For Proper Anterior Regenerationmentioning

confidence: 67%

Localization of planarian β-CATENIN-1 reveals multiple roles during anterior-posterior regeneration and organogenesis

2016

View full text Add to dashboard Cite

The β-catenin-dependent Wnt pathway exerts multiple contextdependent roles in embryonic and adult tissues. In planarians, β-catenin-1 is thought to specify posterior identities through the generation of an anteroposterior gradient. However, the existence of such a gradient has not been directly demonstrated. Here, we use a specific polyclonal antibody to demonstrate that nuclear β-CATENIN-1 exists as an anteroposterior gradient from the pre-pharyngeal region to the tail of the planarian Schmidtea polychroa. High levels in the posterior region steadily decrease towards the pre-pharyngeal region but then increase again in the head region. During regeneration, β-CATENIN-1 is nuclearized in both anterior and posterior blastemas, but the canonical WNT1 ligand only influences posterior nuclearization. Additionally, β-catenin-1 is required for proper anterior morphogenesis, consistent with the high levels of nuclear β-CATENIN-1 observed in this region. We further demonstrate that β-CATENIN-1 is abundant in developing and differentiated organs, and is particularly required for the specification of the germline. Altogether, our findings provide the first direct evidence of an anteroposterior nuclear β-CATENIN-1 gradient in adult planarians and uncover novel, context-dependent roles for β-catenin-1 during anterior regeneration and organogenesis.

show abstract

Section: β-Catenin-1 Is Required For Proper Anterior Regenerationmentioning

confidence: 67%

Localization of planarian β-CATENIN-1 reveals multiple roles during anterior-posterior regeneration and organogenesis

2016

View full text Add to dashboard Cite

show abstract

“…Following sexualization, the triploids develop both gonads and other reproductive organs and begin reproducing by copulation instead of fission. We named these sexualized triploids "acquired sexuals" (Hoshi et al 2003). In the present study, we performed karyological and cytological investigations of the offspring obtained by inbreeding of such acquired sexuals.…”

Section: Introductionmentioning

confidence: 99%

Production of diploid and triploid offspring by inbreeding of the triploid planarian Dugesia ryukyuensis

et al. 2008

View full text Add to dashboard Cite

Triploidy has generally been considered to be an evolutionary dead end due to problems of chromosomal pairing and segregation during meiosis. Thus, the formation of tetraploids and diploids from triploid types is a rare phenomenon. In the present study, we demonstrated that inbreeding of the triploid planarian Dugesia ryukyuensis resulted in both diploid and triploid offspring in nature. In the triploids of D. ryukyuensis, chiasmata between homologous chromosomes were observed in both female and male germ lines. This result suggests that both diploid and triploid offspring of this species are produced bisexually by zygotic fusion between sperm and eggs. Hence, this phenomenon may be a novel mechanism in planarian for escaping the triploid state.

show abstract

“…They are not likely to fission. In the physiological race, worms convert between asexual and sexual reproduction seasonally [6].…”

Section: Introductionmentioning

confidence: 99%

Cloning and characterization of DjPRPS gene in freshwater planarian Dugesia japonica

et al. 2015

View full text Add to dashboard Cite

Objective: To clone the phosphoribosyl pyrophosphate synthetase gene from Dugesia japonica, and to do the bioinformatics and spatial expression analysis of the gene. Methods:The phosphoribosyl pyrophosphate synthetase gene (DjPRPS) was cloned from Dugesia japonica using the rapid amplification of cDNA ends method and its sequence was analyzed with biological softwares. Spatial expression pattern of the gene was detected by whole-mount in situ hybridization. Results:The full length cDNA of DjPRPS was 1,086 bp, containing a 80 bp of 5'-untranslated region, a 55 bp of 3'-untranslated region and a 951 bp of open reading frame encoding a protein of 316 amino acids with a calculated molecular mass of 35.18 kDa and isoelectric point of 8.14. The deduced amino acid sequence shared the conserved eukaryotic PRPS family motif. Phylogenetic analysis supported the traditional concept of zoological taxonomy in large scale. Intense positive signals were detected as bilateral lines of patches in the ventral side where the ovaries and yolk glands located in the intact sexual planarians. In the regenerating planarians, the expression pattern was similar to that of the intact samples, and no intense transcipt signals were detected in the blastema. Conclusion: DjPRPS sequence was highly homologous with that of other eukaryotic phosphoribosyl pyrophosphate synthetase gene. Intense transcript signals of DjPRPS were not detected in the blastema but in ovaries and yolk glands, suggesting that its function was probably reproduction-related and maybe not involved in regeneration. Key Words: Dugesia japonica, DjPRPS, cloning, whole-mount in situ hybridization Conflict of Interest: The authors have no conflict of interest. ÖZETAmaç: Dugesia japonica'dan fosforibozil pirofosfat sentetaz geninin klonlanması ve genin mekansal ekspresyon analizi ile bioinformatik çalışmaların yapılmasıdır. Metod: Fosforibozil pirofosfat sentetaz (DjPRPS) geni Dugesia japonica'dan cDNA ucunun hızlı amplifikasyon metodu kullanılarak klonlandı ve dizilim biyolojik yazılımlar ile analiz edildi. Genin mekansal ekspresyon motifi whole-mount in situ hibridizasyon ile saptandı. Bulgular: DjPRPS geninin tüm cDNA uzunluğu 1,086 bp'dir. Bu yapı, 80 bp 5'-çevrilmemiş bölge, 55 bp 3'-çevrilmemiş bölge ve 951 bp'lik izoelektrik noktası 8.14 ve hesaplanan moleküler kütlesi 35.18 kDa olan 316 amino asitden oluşan proteini kodlayan açık okuma ucu içe-rir. Ortaya çıkarılan amino asit dizilimi korunmuş ökaryotik PRPS ailesi motifini paylaşmıştır. Filogenetik analizler büyük oranda geleneksel zoolojik taksonomi kavramlarını desteklemiştir. Bozulmamış seksüel planaryalarda yerleşmiş yumurtalık ve yolk keselerinde ventral bölgede yoğunlaştırılmış pozitif sinyaller bilateral yama tarzında çizgiler olarak tespit edildi. Rejenere olan planaryalarda da ifade edilme kalıbı benzerdi ve embriyonik hücre gruplarında yoğun transkripsiyon sinyalleri tespit edilmedi. Sonuç: DjPRPS gen dizilimi diğer ökaryotik fosforibozil pirofosfat sentetaz geni ile yüksek oranda homoloji göstermekte...

show abstract

Switch from Asexual to Sexual Reproduction in the Planarian Dugesia ryukyuensis

Cited by 28 publications

References 15 publications

Localization of planarian β-CATENIN-1 reveals multiple roles during anterior-posterior regeneration and organogenesis

Localization of planarian β-CATENIN-1 reveals multiple roles during anterior-posterior regeneration and organogenesis

Production of diploid and triploid offspring by inbreeding of the triploid planarian Dugesia ryukyuensis

Cloning and characterization of DjPRPS gene in freshwater planarian Dugesia japonica

Contact Info

Product

Resources

About