Swiss Population Data for 3 STR-Systems (SE33, HUMTHO1, D21S11), HLA DQα and D1S80

Krätzer, A.; Gränacher, A.; Jamnicki, Marina; Bär, W.

doi:10.1007/978-3-642-78782-9_142

Cited by 8 publications

(1 citation statement)

References 7 publications

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“…The nomenclature proposed by Schmitter and Sonntag (1995) is unwieldy and does not accurately reflect the number of repeats as recommended by the ISFH (1994). The nomenclature of Kratzer et al (1994) does not consider information on the allele sequences. The nomenclature proposed by Möller et al (1995) while accurately reflecting the repeat structure, appears not to have found favour with the forensic community due to its relative complexity.…”

Section: Resultsmentioning

confidence: 99%

ACTBP2-nomenclature recommendations of GEDNAP

Schneider

Rand

Schmitter

et al. 1998

International Journal of Legal Medicine

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A system of nomenclature is proposed for the complex STR system ACTBP2 (SE33) in order to facilitate data exchange between laboratories. The nomenclature conforms to the ISFH recommendations as far as it is possible for such complex systems. A blind trial was carried out between up to 20 laboratories to ascertain the reproducibility of the nomenclature under working conditions. The population studies carried out have established that there are minimal regional differences in the allele frequencies and that the system of nomenclature is robust.

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Section: Resultsmentioning

confidence: 99%

ACTBP2-nomenclature recommendations of GEDNAP

Schneider

Rand

Schmitter

et al. 1998

International Journal of Legal Medicine

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Forensic validation of the short tandem repeat HUMACTBP2 using capillary electrophoresis

Dimo-Simonin¹,

Grange²,

Krätzer³

et al. 1998

Electrophoresis

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Experiments were performed to evaluate the forensic identification of the short tandem repeat (STR) HUMACTBP2 (human beta-actin-related pseudogene) using automated fluorescence-based capillary electrophoresis. The HUMACTBP2 is a complex tetranucleotide STR locus with more than 32 alleles in the range of 202-323 bp. The reproducibility of genetic typing using a fluorescent labeled allelic ladder was determined by comparison of the calculated fragment size after consecutive (within-day) and nonconsecutive (day to day) injection. The maximum variation in size (window) observed for any allele was 0.23 bp for the within-day and 0.8 bp for the day-to-day precision. Furthermore, it is possible to achieve a 1 bp resolution, the precision of the reproducibility assays being about 99.95%. Sixty blood samples and twenty stains were typed with both automated fluorescent sequencer ABI 373A and ABI 310. Identical genotypes were obtained with both techniques and the ABI 310 seemed to be more sensitive than the ABI 373A. A population sample of 197 unrelated individuals from southwest Switzerland was analyzed and the genotype frequencies observed were similar to those reported by others. Thirty-one alleles and 126 genotypes were found. The observed heterozygosity was 0.934. Mixtures from two different blood samples varying in their ratio were typed and the minor fraction was detectable to about 1:10. The practical usefulness of the HUMACTBP2 is illustrated by analyzing casework samples. This validation study proves the usefulness of the HUMACTBP2 locus in forensics and the detection efficiency using fluorescent capillary electrophoresis.

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