Swine Hemi-Facial Composite Tissue Allotransplantation: A Model to Study Immune Rejection

Kuo, Yur‐Ren; Shih, Hsiang Shun; Lin, Chien Chih; Huang, Chung Cheng; Yang, Johnson Chia Shen; Wu, Wen Chung; Goto, Shigeru; Chen, Chao Long; Lee, W. P.Andrew

doi:10.1016/j.jss.2008.03.050

Cited by 31 publications

(16 citation statements)

References 23 publications

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“…Orthotopic hemi-facial allotransplantation (Hwa-Ban strain to Lan-Yu strain) was performed as described previously [7]. The swine hemi-facial transplantation tissue consisted of ear cartilage, auricular nerve, parotid gland, lymphoid tissue, and muscle with surrounding hemi-facial skin paddle [7].…”

Section: Methodsmentioning

confidence: 99%

“…The swine hemi-facial transplantation tissue consisted of ear cartilage, auricular nerve, parotid gland, lymphoid tissue, and muscle with surrounding hemi-facial skin paddle [7]. The vascular territories of the composite allotransplant supplied by the superficial temporal artery and its branches originating from the carotid artery were defined.…”

Section: Methodsmentioning

confidence: 99%

“…Our results led us to speculate that BMT is unnecessary to prolong CTA survival if MSCs are used as an immunosuppressant [5]. The miniature swine hemi-facial CTA model (consisting of skin paddle, muscle, ear cartilage, and lymphoid gland tissue) had been established by our institute recently [7]. The difference between the hemifacial CTA model and the hind-limb model is that this hemi-facial model does not contain donor vascularized bone, but it does include more alloskin area and lymphoid gland tissue.…”

Section: Introductionmentioning

confidence: 94%

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Immunomodulatory Effects of Bone Marrow-Derived Mesenchymal Stem Cells in a Swine Hemi-Facial Allotransplantation Model

et al. 2012

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BackgroundIn this study, we investigated whether the infusion of bone marrow-derived mesenchymal stem cells (MSCs), combined with transient immunosuppressant treatment, could suppress allograft rejection and modulate T-cell regulation in a swine orthotopic hemi-facial composite tissue allotransplantation (CTA) model.Methodology/Principal FindingsOutbred miniature swine underwent hemi-facial allotransplantation (day 0). Group-I (n = 5) consisted of untreated control animals. Group-II (n = 3) animals received MSCs alone (given on days −1, +1, +3, +7, +14, and +21). Group-III (n = 3) animals received CsA (days 0 to +28). Group-IV (n = 5) animals received CsA (days 0 to +28) and MSCs (days −1, +1, +3, +7, +14, and +21). The transplanted face tissue was observed daily for signs of rejection. Biopsies of donor tissues and recipient blood sample were obtained at specified predetermined times (per 2 weeks post-transplant) or at the time of clinically evident rejection. Our results indicated that the MSC-CsA group had significantly prolonged allograft survival compared to the other groups (P<0.001). Histological examination of the MSC-CsA group displayed the lowest degree of rejection in alloskin and lymphoid gland tissues. TNF-α expression in circulating blood revealed significant suppression in the MSC and MSC-CsA treatment groups, as compared to that in controls. IHC staining showed CD45 and IL-6 expression were significantly decreased in MSC-CsA treatment groups compared to controls. The number of CD4+/CD25+ regulatory T-cells and IL-10 expressions in the circulating blood significantly increased in the MSC-CsA group compared to the other groups. IHC staining of alloskin tissue biopsies revealed a significant increase in the numbers of foxp3+T-cells and TGF-β1 positive cells in the MSC-CsA group compared to the other groups.ConclusionsThese results demonstrate that MSCs significantly prolong hemifacial CTA survival. Our data indicate the MSCs did not only suppress inflammation and acute rejection of CTA, but also modulate T-cell regulation and related cytokines expression.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 94%

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Immunomodulatory Effects of Bone Marrow-Derived Mesenchymal Stem Cells in a Swine Hemi-Facial Allotransplantation Model

et al. 2012

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“…[6][7][8][9] Other models used have been rats, [10][11][12][13][14] rabbits and dogs, [15,16] animals that are easy to use in a research center [ Figure 3]. [17,18] Preclinical studies on cadavers have sought to find the best way to recover soft and hard tissues, muscles, nerves and vessels of donor face while reducing tissue ischemia to the minimum.…”

Section: Surgical Considerations Preclinical Modelsmentioning

confidence: 99%

Current role in facial allograft transplantation: what have we learned?

Infante-Cossio¹,

Barrera-Pulido²,

Gómez-Cía³

et al. 2016

PAR

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Face transplant (FT) has evolved enormously in the last 10 years since the successful completion of the first facial transplant. This procedure has become a new reconstructive option for complex facial deformities to restore the anatomy of patients with severely disfigured faces. The authors review the literature and discuss the main surgical, immunological, and ethical aspects as well as the results described in patients undergoing FT. To date there have been more than thirty FT worldwide. The main indication was post-traumatic deformity. In all cases a standard immunosuppression was performed with three drugs, although acute rejection episodes were observed, that could be controlled with conventional immunosuppressive regimen. Overall, functional and aesthetic results have been excellent at short-term and high satisfaction rate exceeded initial expectations, although long-term data are still scarce. Major complications were opportunistic infections. Five deaths that occurred have reopened the ethical debate about the potential complications and concerns of providing informed consent to recipients. Continuous progresses in microsurgical techniques and preoperative planning have promoted the evolution from partial to full FT. All these are on the basis of accurate and careful selection of wellmotivated candidates. The next challenge will be getting new immunosuppressive treatment strategies. Although clinical experience has demonstrated the FT viability, it is still considered an experimental procedure in which we have much to learn to define its true role in the current reconstructive surgery and resolve major technical, medical and ethical problems involved.

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“…"Composite tissue allotransplantation" spielt auch in der plastischen und rekons truktiven Chirurgie zunehmend eine Rol le [19,30]. Neben Hand und Unterarm transplantationen sind insbesondere Teil gesichtsverpflanzungen von einer brei ten Öffentlichkeit wahrgenommen wor den.…”

Section: Cta In Der Plastischen Chirurgieunclassified

Organtransplantation, Gewebetransplantation und plastische Chirurgie

2010

J. f. Ästhet. Chirurgie

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Swine Hemi-Facial Composite Tissue Allotransplantation: A Model to Study Immune Rejection

Cited by 31 publications

References 23 publications

Immunomodulatory Effects of Bone Marrow-Derived Mesenchymal Stem Cells in a Swine Hemi-Facial Allotransplantation Model

Immunomodulatory Effects of Bone Marrow-Derived Mesenchymal Stem Cells in a Swine Hemi-Facial Allotransplantation Model

Current role in facial allograft transplantation: what have we learned?

Organtransplantation, Gewebetransplantation und plastische Chirurgie

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