Swine Dysentery: Induction and Characterization in Isolated Colonic Loops

Hughes, R. E.; Olander, H. J.; Gallina, A. M.; Morrow, Marie E.

doi:10.1177/030098587200900103

Cited by 8 publications

(5 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An early lesion in the pathogenesis of swine dysentery is catarrhal enteritis (18), and since the disease is not produced in germfree pigs with T. hyodysenteriae, it has now been suggested that synergism between T. hyodysenteriae and a portion of the normal enteric flora occurs in swine dysentery (16). As in the present study, increased numbers of vibrios and spirochetes other than T. hyodysenteriae have been observed in swine dysentery (22,23); however, the presence of large numbers of a particular organism in diarrheal disease does not necessarily reflect its pathogenicity (15,25). In addition, the controversy regarding the pathogenic role of T. trichiura in man may be explained by an interaction between the nematode and the gut flora; although Jung and Beaver (24) observed that Entamoeba histolytica was frequently present in association with human trichuriasis, T. trichiura has also been reported to be pathogenic in the absence of E. histolytica (10).…”

Section: W~-supporting

confidence: 67%

Synergism between Trichuris suis and the microbial flora of the large intestine causing dysentery in pigs

Rutter¹,

Beer²

1975

Infect Immun

View full text Add to dashboard Cite

The role of the microbial flora of the large intestine in experimental Trichuris suis infection was studied by comparing the clinical syndrome in conventionally reared (CR) pigs, specific pathogen-free pigs, and gnotobiotic pigs. Thedisease in CR pigs was characterized by a severe mucohemorrhagic enteritis; in contrast, a mild catarrhal enteritis was observed in specific pathogen-free and gnotobiotic pigs.Spirochaetes and vibrio-like organisms were observed only in CR pigs and increased during the clinical phase of the disease. The clinical syndrome was not transmitted by oral administration of intestinal or fecal material from infected CR pigs to CR pigs free of T. suis. Smaller numbers of T. suis produced diarrhea in CR pigs and significantly reduced the growth rates of infected animals; clinical signs and the reduction in growth rate was prevented by incorporating an antibacterial substance (dimetridazole) in the food. Although clinical trichuriasis closely resembles swin dysentery, the two syndromes seem to be distinct. The present results suggest that a microbial component acts synergistically with T. suis to produce the severe clinical syndrome in CR pigs, but identification of the microbial component and the mechanism by which clinical signs are produced await further studies of the bacterial flora of the large intestine of pigs.

show abstract

Section: W~-supporting

confidence: 67%

Synergism between Trichuris suis and the microbial flora of the large intestine causing dysentery in pigs

Rutter¹,

Beer²

1975

Infect Immun

View full text Add to dashboard Cite

show abstract

“…The rapid proliferation of opportunistic organisms [17] may have been triggered by the leakage of serum and hemorrhage from a damaged mucosa. These organisms may play a major role in the production of the fibrinonecrotic membrane found later in the course of the disease.…”

Section: Discussionmentioning

confidence: 99%

A Study of Swine Dysentery by Immunofluorescence and Histology

et al. 1977

Self Cite

View full text Add to dashboard Cite

Abstract. Twenty-six specific-pathogen-free pigs were fed pure cultures of Treponemu hyodysenteriue. Five untreated pigs were controls. Distribution of this large spirochete in pigs with swine dysentery was shown by the indirect fluorescent antibody technique. Findings by this method were compared with those from dark-field examination of colonic mucosal scrapings and from tissue sections. The cultures caused mucohemorrhagic colitis which by 10 days after inoculation was indistinguishable from the colitis of swine dysentery. Control pigs remained normal. Pigs killed when spirochetes were first seen in their feces had normal colonic mucosa with only a few spirochetes. At the first sign of diarrhea, however, the colonic mucosa was thicker than normal and had many spirochetes. T. hyodysenreriue was confined to regions of hypertrophy and exudation of the large intestine mucosa throughout the course of disease.Culture studies, dark-field microscopy, special staining techniques and electron microscopy have suggested a relationship between T. hyodysenreriue and swine dysentery [14][15][16]. Observational limitations, however, have precluded correlation of the findings with the development of the disease. Culture and dark-field observations place the organisms only in the gross vicinity of the lesion; special staining techniques do not identify organisms specifically, and electron microscopy gives a very selective view of the morphologic relationship between the organisms and the disease. We used specific immunofluorescent staining of T. hyodysenreriue in the affected tissues to correlate the presence of the organisms with the development of the lesions.A mucohemorrhagic diarrhea disease of pigs was shown to be distinct from hog cholera and salmonellosis in 1921 and was named swine dysentery [26]. A large spirochete was associated with the lesions but feeding a mixed culture containing the organism did not produce the disease. A comma-shaped organism also was seen in swine dysentery at that time and was later isolated and named Vibrio coli. This was considered the cause of the disease [9-111. Although V. coli was generally accepted to be the cause of swine dysentery [4,7, 10, 111, this organism has been commonly found in normal pigs [2, 8, 131, and in 490

show abstract

“…Since the large spirochaete Treponema hyodysenteriae was implicated in swine dysentery (SD) (Taylor & Alexander, 1971;Harris et al 1972), it has become a matter of diagnostic importance to be able to distinguish it from other spirochaetes which occur in the porcine alimentary tract. The most reliable method of identification is testing for enteropathogenicity in conventionally reared pigs (Kinyon, Harris & Glock, 1977), or in isolated colonic loops (Hughes et at. 1972;Whipp et al 1978;Wilcock & Olander, 1979).…”

Section: Introductionmentioning

confidence: 99%

A comparative study of spirochaetes from the porcine alimentary tract

Lemcke

Burrows

1981

J. Hyg.

View full text Add to dashboard Cite

Strains of Treponema hyodysenteriae capable of inducing swine dysentery in specific pathogen-free pigs were compared with other spirochaetes from the porcine alimentary tract by biochemical and serological tests and by electrophoresis of their proteins. Carbohydrate fermentation and esculin hydrolysis were similar in all the spirochaetes. Indole was produced by T. hyodysenteriae and by some of the other spirochaetes. Analysis of the fatty acids produced from glucose showed a difference between T. hyodysenteriae and other spirochaetes only in the amount of n-butyric acid produced. The indirect fluorescent antibody test showed extensive cross-reactions between all the spirochaetes unless antisera were first absorbed. A microtitre agglutination test and a growth-inhibition test were both more specific; strains of T. hyodysenteriae could be distinguished from the other spirochaetes using unabsorbed sera. Both tests revealed some antigenic heterogeneity among strains of T, hyodysenteriae. The cell proteins of a single strain of T. hyodysenteriae gave an electrophoretic pattern distinct from those of the other spirochaetes. Two of the six spirochaetes not associated with swine dysentery, PWS/B and PWS/C, were indistinguishable serologically and electrophoretically. The other four strains were serologically distinct from one another and from PWS/B and PWS/C. Only two of these spirochaetes were examined electrophoretically, but each gave a different pattern from PWS/B and PWS/C. The diversity observed among spirochaetes not associated with swine dysentery indicates that their suggested inclusion in a single species, T. innocens, may prove to be unjustified.

show abstract

Swine Dysentery: Induction and Characterization in Isolated Colonic Loops

Cited by 8 publications

References 4 publications

Synergism between Trichuris suis and the microbial flora of the large intestine causing dysentery in pigs

Synergism between Trichuris suis and the microbial flora of the large intestine causing dysentery in pigs

A Study of Swine Dysentery by Immunofluorescence and Histology

A comparative study of spirochaetes from the porcine alimentary tract

Contact Info

Product

Resources

About